Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_004537                                                              17    4312
NP_064508                                                              16    5631
NP_055906                                                              16    5631
NP_542119                                                              16    5631
NP_110412                                                              16    5631
NP_001724                                                              16    5631
XP_005267508                                                           16    5631
NP_077313                                                              16    7355
YP_003024028                                                           16    7355
NP_000949                                                              15    9605
NP_001367                                                              15    9605
NP_055951                                                              15    9605
NP_001348                                                              15    9605
NP_803879                                                              15    9605
NP_803881                                                              15    9605
NP_001258992                                                           15    9605
NP_110428                                                              15    9605
NP_001229291                                                           15    9605
NP_001258991                                                           15    9605
NP_001092270                                                           15    9605
NP_001258990                                                           15    9605
NP_114154                                                              15    9605
NP_060890                                                              15    9605
XP_006725230                                                           15    9605
XP_006724671                                                           15    9605
XP_006724670                                                           15    9605
XP_005255674                                                           15    9605
XP_006720283                                                           15    9605
XP_006713129                                                           15    9605
XP_006713128                                                           15    9605
XP_005265032                                                           15    9605
XP_005265031                                                           15    9605
XP_005265030                                                           15    9605
XP_006710817                                                           15    9605
XP_005271062                                                           15    9605
XP_006710816                                                           15    9605
NP_065869                                                              15    9605
NP_542174                                                              15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALFARMGGQ 9
            AL ARMGGQ
Sbjct: 3738 ALLARMGGQ 3746
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALFARMGGQ 9
            AL ARMGGQ
Sbjct: 3730 ALLARMGGQ 3738
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALFARMGGQ 9
            AL ARMGGQ
Sbjct: 3691 ALLARMGGQ 3699
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALFARMGGQ 9
            AL ARMGGQ
Sbjct: 3770 ALLARMGGQ 3778
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALFARMGGQ 9
            AL ARMGGQ
Sbjct: 3783 ALLARMGGQ 3791
>NP_004537
          Length = 105

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ALFARMGGQ 9
          A FAR+GG+
Sbjct: 8  ASFARVGGR 16
>NP_064508
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALFARMGGQ 9
           +L+AR GG+
Sbjct: 345 SLYARQGGR 353
>NP_055906
          Length = 1720

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALFARMG 7
            ALFA+MG
Sbjct: 1684 ALFAQMG 1690
>NP_542119
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LFARMGGQ 9
          +FARMG Q
Sbjct: 27 MFARMGFQ 34
>NP_110412
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ALFARMGG 8
           ALF R GG
Sbjct: 277 ALFTRSGG 284
>NP_001724
          Length = 705

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  ALFARMGG 8
          ALF R GG
Sbjct: 10 ALFCRAGG 17
>XP_005267508
          Length = 1773

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALFARMG 7
            ALFA+MG
Sbjct: 1737 ALFAQMG 1743
>NP_077313
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ALFARMGGQ 9
          +LF++MG Q
Sbjct: 87 SLFSQMGSQ 95
>YP_003024028
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALFARMGG 8
           A+FA MGG
Sbjct: 385 AVFAIMGG 392
>NP_000949
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   LFARMGG 8
           LF R+GG
Sbjct: 347 LFCRIGG 353
>NP_001367
          Length = 4646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    ALFARMGG 8
            AL AR+GG
Sbjct: 1895 ALEARLGG 1902
>NP_055951
          Length = 2570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>NP_001348
          Length = 1270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ARMGGQ 9
           ARMGG+
Sbjct: 928 ARMGGE 933
>NP_803879
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_803881
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_001258992
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_110428
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_001229291
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_001258991
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_001092270
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_001258990
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>NP_114154
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ARMGGQ 9
           AR+GGQ
Sbjct: 284 ARLGGQ 289
>NP_060890
          Length = 547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ALFARMGG 8
           ++F+R+GG
Sbjct: 439 SMFSRVGG 446
>XP_006725230
          Length = 2636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FARMGG 8
           FAR+GG
Sbjct: 584 FARVGG 589
>XP_006724671
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>XP_006724670
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FARMGGQ 9
           +ARMGG+
Sbjct: 338 WARMGGK 344
>XP_005255674
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ARMGGQ 9
           AR+GGQ
Sbjct: 182 ARLGGQ 187
>XP_006720283
          Length = 2636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FARMGG 8
           FAR+GG
Sbjct: 584 FARVGG 589
>XP_006713129
          Length = 1521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>XP_006713128
          Length = 1619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>XP_005265032
          Length = 2545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>XP_005265031
          Length = 2569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>XP_005265030
          Length = 2595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    ARMGGQ 9
            AR+GGQ
Sbjct: 1067 ARLGGQ 1072
>XP_006710817
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ALFARMGG 8
           ++F+R+GG
Sbjct: 207 SMFSRVGG 214
>XP_005271062
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ALFARMGG 8
           ++F+R+GG
Sbjct: 208 SMFSRVGG 215
>XP_006710816
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ALFARMGG 8
           ++F+R+GG
Sbjct: 368 SMFSRVGG 375
>NP_065869
          Length = 2458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FARMGG 8
           FAR+GG
Sbjct: 406 FARVGG 411
>NP_542174
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ALFARMGG 8
          ALF ++GG
Sbjct: 16 ALFLQLGG 23
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.144    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 565,003
Number of extensions: 831
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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