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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001157862                                                           16    7355
NP_001157861                                                           16    7355
NP_001157860                                                           16    7355
NP_001157859                                                           16    7355
NP_001157858                                                           16    7355
NP_057424                                                              16    7355
NP_005379                                                              15    9605
NP_001167448                                                           15    9605
NP_060110                                                              15    9605
NP_037479                                                              15    9605
NP_055613                                                              15    9605
XP_006715201                                                           15    9605
XP_006715200                                                           15    9605
XP_005249275                                                           15    9605
XP_006715199                                                           15    9605

>NP_001157862
          Length = 827

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_001157861
          Length = 1391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_001157860
          Length = 1504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_001157859
          Length = 1509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_001157858
          Length = 1609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_057424
          Length = 1601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EVVMVH 8
           E+VMVH
Sbjct: 395 EIVMVH 400



>NP_005379
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VVMVHI 9
           V+MVHI
Sbjct: 182 VIMVHI 187



>NP_001167448
          Length = 1365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 945 ALEEVPIHI 953



>NP_060110
          Length = 1371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 945 ALEEVPIHI 953



>NP_037479
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ALEVVMVHI 9
           +LEV+ +H+
Sbjct: 139 SLEVLKIHV 147



>NP_055613
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  ALEVVMVH 8
          ALE V +H
Sbjct: 53 ALEAVFIH 60



>XP_006715201
          Length = 1375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 949 ALEEVPIHI 957



>XP_006715200
          Length = 1419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 949 ALEEVPIHI 957



>XP_005249275
          Length = 1421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 945 ALEEVPIHI 953



>XP_006715199
          Length = 1425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEVVMVHI 9
           ALE V +HI
Sbjct: 949 ALEEVPIHI 957



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.143    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,120
Number of extensions: 247
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)