Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_066997                                                              16    5631
NP_054878                                                              16    5631
NP_644814                                                              16    5631
XP_006721859                                                           16    5631
XP_005265151                                                           16    5631
XP_006713183                                                           16    5631
XP_006713182                                                           16    5631
NP_001288                                                              16    7355
NP_001273059                                                           16    7355
XP_006721197                                                           16    7355
NP_116250                                                              15    9605
NP_006280                                                              15    9605
NP_001273497                                                           15    9605
NP_115519                                                              15    9605
NP_001020029                                                           15    9605
NP_001273498                                                           15    9605
NP_006071                                                              15    9605
NP_000338                                                              15    9605
NP_892018                                                              15    9605
XP_005259608                                                           15    9605
XP_006720304                                                           15    9605
XP_005268080                                                           15    9605
XP_006716915                                                           15    9605
XP_005251621                                                           15    9605
XP_006715902                                                           15    9605
XP_006715901                                                           15    9605
XP_005250168                                                           15    9605
XP_005250167                                                           15    9605
XP_006715649                                                           15    9605
XP_006715648                                                           15    9605
XP_005263869                                                           15    9605
XP_005263868                                                           15    9605
XP_006712851                                                           15    9605
XP_005263866                                                           15    9605
>NP_066997
          Length = 923

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EATTKQE 9
           EATT+QE
Sbjct: 486 EATTQQE 492
>NP_054878
          Length = 2564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           +LEA TKQ+
Sbjct: 249 SLEADTKQD 257
>NP_644814
          Length = 880

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ALEATTK 7
           A+EATTK
Sbjct: 442 AIEATTK 448
>XP_006721859
          Length = 844

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ALEATTK 7
           A+EATTK
Sbjct: 406 AIEATTK 412
>XP_005265151
          Length = 2058

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           +LEA TKQ+
Sbjct: 249 SLEADTKQD 257
>XP_006713183
          Length = 2258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           +LEA TKQ+
Sbjct: 249 SLEADTKQD 257
>XP_006713182
          Length = 2422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           +LEA TKQ+
Sbjct: 249 SLEADTKQD 257
>NP_001288
          Length = 1251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    ALEATTKQE 9
            ALEA  KQ+
Sbjct: 1141 ALEAAAKQQ 1149
>NP_001273059
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    ALEATTKQE 9
            ALEA  KQ+
Sbjct: 1135 ALEAAAKQQ 1143
>XP_006721197
          Length = 1251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    ALEATTKQE 9
            ALEA  KQ+
Sbjct: 1141 ALEAAAKQQ 1149
>NP_116250
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LEATTKQE 9
           LEA+TK E
Sbjct: 509 LEASTKPE 516
>NP_006280
          Length = 2541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALEATTK 7
            ALEATT+
Sbjct: 2145 ALEATTE 2151
>NP_001273497
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 814 ALEEITKQQ 822
>NP_115519
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 816 ALEEITKQQ 824
>NP_001020029
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LEATTKQE 9
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371
>NP_001273498
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 362 ALEEITKQQ 370
>NP_006071
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           A+E +TKQ+
Sbjct: 491 AMELSTKQQ 499
>NP_000338
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LEATTKQE 9
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371
>NP_892018
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LEATTK 7
           LEATTK
Sbjct: 432 LEATTK 437
>XP_005259608
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LEATTK 7
           LEATTK
Sbjct: 466 LEATTK 471
>XP_006720304
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LEATTKQE 9
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371
>XP_005268080
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    LEATTKQE 9
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371
>XP_006716915
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALEATTK 7
            ALEATT+
Sbjct: 2145 ALEATTE 2151
>XP_005251621
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALEATTK 7
            ALEATT+
Sbjct: 2145 ALEATTE 2151
>XP_006715902
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           A+E +TKQ+
Sbjct: 491 AMELSTKQQ 499
>XP_006715901
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           A+E +TKQ+
Sbjct: 491 AMELSTKQQ 499
>XP_005250168
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           A+E +TKQ+
Sbjct: 491 AMELSTKQQ 499
>XP_005250167
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALEATTKQE 9
           A+E +TKQ+
Sbjct: 491 AMELSTKQQ 499
>XP_006715649
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LEATTKQE 9
           LEA+TK E
Sbjct: 439 LEASTKPE 446
>XP_006715648
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LEATTKQE 9
           LEA+TK E
Sbjct: 471 LEASTKPE 478
>XP_005263869
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 756 ALEEITKQQ 764
>XP_005263868
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 756 ALEEITKQQ 764
>XP_006712851
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 816 ALEEITKQQ 824
>XP_005263866
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALEATTKQE 9
           ALE  TKQ+
Sbjct: 816 ALEEITKQQ 824
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.286   0.0997    0.220 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,814
Number of extensions: 571
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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