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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055714                                                              19     869
NP_000258                                                              18    1482
NP_001035957                                                           18    1482
XP_006721990                                                           18    1482
XP_006721989                                                           18    1482
XP_005258041                                                           18    1482
XP_006721988                                                           18    1482
XP_006721987                                                           18    1482
XP_006721986                                                           18    1482
XP_005258040                                                           18    1482
XP_006721985                                                           18    1482
NP_057319                                                              17    4312
XP_006721115                                                           17    4312
XP_006721114                                                           17    4312
XP_005255414                                                           17    4312
NP_055786                                                              16    5631
NP_001264044                                                           16    5631
NP_001091742                                                           16    5631
NP_001192222                                                           16    5631
NP_001192223                                                           16    5631
NP_000712                                                              16    5631
NP_000102                                                              16    5631
NP_003881                                                              16    5631
NP_002121                                                              16    5631
NP_919278                                                              16    5631
XP_006715936                                                           16    5631
XP_005248352                                                           16    5631
XP_006712459                                                           16    5631

>NP_055714
          Length = 611

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALAFGNVKL 9
          A AFGNVKL
Sbjct: 60 ASAFGNVKL 68



>NP_000258
          Length = 2818

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2016 ALASGNVKL 2024



>NP_001035957
          Length = 2839

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2037 ALASGNVKL 2045



>XP_006721990
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2047 ALASGNVKL 2055



>XP_006721989
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2047 ALASGNVKL 2055



>XP_005258041
          Length = 2836

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2016 ALASGNVKL 2024



>XP_006721988
          Length = 2846

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2026 ALASGNVKL 2034



>XP_006721987
          Length = 2849

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2047 ALASGNVKL 2055



>XP_006721986
          Length = 2854

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2034 ALASGNVKL 2042



>XP_005258040
          Length = 2857

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2037 ALASGNVKL 2045



>XP_006721985
          Length = 2867

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALA GNVKL
Sbjct: 2047 ALASGNVKL 2055



>NP_057319
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FGNVKL 9
           FGNVKL
Sbjct: 256 FGNVKL 261



>XP_006721115
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FGNVKL 9
           FGNVKL
Sbjct: 256 FGNVKL 261



>XP_006721114
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FGNVKL 9
           FGNVKL
Sbjct: 256 FGNVKL 261



>XP_005255414
          Length = 534

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FGNVKL 9
           FGNVKL
Sbjct: 387 FGNVKL 392



>NP_055786
          Length = 817

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AFGNVK 8
           AFGN+K
Sbjct: 244 AFGNIK 249



>NP_001264044
          Length = 4516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    ALAFGNVKL 9
            ALAFG+V L
Sbjct: 3531 ALAFGDVIL 3539



>NP_001091742
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AFGNVKL 9
           AFG+VKL
Sbjct: 300 AFGDVKL 306



>NP_001192222
          Length = 2313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FGNVKL 9
            FGN+KL
Sbjct: 1629 FGNIKL 1634



>NP_001192223
          Length = 2251

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FGNVKL 9
            FGN+KL
Sbjct: 1610 FGNIKL 1615



>NP_000712
          Length = 2270

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FGNVKL 9
            FGN+KL
Sbjct: 1629 FGNIKL 1634



>NP_000102
          Length = 764

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALAFGNVK 8
           ALA GN+K
Sbjct: 442 ALALGNLK 449



>NP_003881
          Length = 5405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ALAFGN 6
            ALAFGN
Sbjct: 1025 ALAFGN 1030



>NP_002121
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AFGNVKL 9
           AFG+VKL
Sbjct: 300 AFGDVKL 306



>NP_919278
          Length = 1925

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LAFGNV 7
            LAFGNV
Sbjct: 999  LAFGNV 1004



>XP_006715936
          Length = 764

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALAFGNVK 8
           ALA GN+K
Sbjct: 442 ALALGNLK 449



>XP_005248352
          Length = 478

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AFGNVKL 9
           AFG+VKL
Sbjct: 258 AFGDVKL 264



>XP_006712459
          Length = 1914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LAFGNV 7
           LAFGNV
Sbjct: 988 LAFGNV 993



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.143    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,295
Number of extensions: 579
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)