Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           18    1482
NP_001020014                                                           18    1482
NP_055903                                                              18    1482
NP_036443                                                              17    3301
XP_006717498                                                           17    3301
XP_006717497                                                           17    3301
NP_001171661                                                           17    4312
NP_004245                                                              17    4312
NP_001171665                                                           17    4312
NP_001171662                                                           17    4312
NP_054859                                                              16    7355
NP_001120679                                                           16    7355
NP_005903                                                              16    7355
NP_001092909                                                           16    7355
NP_071332                                                              16    7355
XP_006712620                                                           16    7355
XP_005246688                                                           16    7355
XP_005246689                                                           16    7355
NP_006460                                                              15    9605
NP_003119                                                              15    9605
XP_005264575                                                           15    9605
XP_006712150                                                           15    9605
XP_005264574                                                           15    9605
XP_005244900                                                           15    9605
NP_003202                                                              15    9605
NP_842565                                                              15    9605
>NP_001155973
          Length = 1833

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AKRVAVLNG 9
          AKRVAV NG
Sbjct: 70 AKRVAVPNG 78
>NP_001020014
          Length = 1029

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRVAV NG
Sbjct: 106 AKRVAVPNG 114
>NP_055903
          Length = 1723

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AKRVAVLNG 9
          AKRVAV NG
Sbjct: 70 AKRVAVPNG 78
>NP_036443
          Length = 393

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           KR+ VLNG
Sbjct: 341 KRILVLNG 348
>XP_006717498
          Length = 288

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           KR+ VLNG
Sbjct: 235 KRILVLNG 242
>XP_006717497
          Length = 394

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           KR+ VLNG
Sbjct: 341 KRILVLNG 348
>NP_001171661
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           +RVAV NG
Sbjct: 277 RRVAVFNG 284
>NP_004245
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           +RVAV NG
Sbjct: 277 RRVAVFNG 284
>NP_001171665
          Length = 419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           +RVAV NG
Sbjct: 154 RRVAVFNG 161
>NP_001171662
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           +RVAV NG
Sbjct: 186 RRVAVFNG 193
>NP_054859
          Length = 954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRV +L+G
Sbjct: 572 AKRVILLSG 580
>NP_001120679
          Length = 954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRV +L+G
Sbjct: 572 AKRVILLSG 580
>NP_005903
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KRVAVLNG 9
           KR+AVL G
Sbjct: 224 KRIAVLPG 231
>NP_001092909
          Length = 1008

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVAVLNG 9
           RVA LNG
Sbjct: 424 RVAALNG 430
>NP_071332
          Length = 384

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KRVAVLN 8
           KRV+VLN
Sbjct: 224 KRVSVLN 230
>XP_006712620
          Length = 932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRV +L+G
Sbjct: 550 AKRVILLSG 558
>XP_005246688
          Length = 954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRV +L+G
Sbjct: 572 AKRVILLSG 580
>XP_005246689
          Length = 954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKRVAVLNG 9
           AKRV +L+G
Sbjct: 572 AKRVILLSG 580
>NP_006460
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VAVLNG 9
           VAVLNG
Sbjct: 554 VAVLNG 559
>NP_003119
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKRVAVLN 8
           A RVAV+N
Sbjct: 902 ASRVAVVN 909
>XP_005264575
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKRVAVLN 8
           A RVAV+N
Sbjct: 889 ASRVAVVN 896
>XP_006712150
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKRVAVLN 8
           A RVAV+N
Sbjct: 902 ASRVAVVN 909
>XP_005264574
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKRVAVLN 8
           A RVAV+N
Sbjct: 902 ASRVAVVN 909
>XP_005244900
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VAVLNG 9
           VAVLNG
Sbjct: 336 VAVLNG 341
>NP_003202
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RVAVLNG 9
           R+AV NG
Sbjct: 225 RIAVFNG 231
>NP_842565
          Length = 2155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKRVAVLN 8
           A RVAV+N
Sbjct: 889 ASRVAVVN 896
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.132    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,805
Number of extensions: 529
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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