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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              21     229
NP_444254                                                              21     229
NP_444253                                                              21     229
NP_444255                                                              21     229
XP_005247551                                                           21     229
XP_005247550                                                           21     229
XP_006713712                                                           21     229
XP_005247549                                                           21     229
NP_000095                                                              18    1935
NP_005957                                                              17    4312
XP_005246644                                                           17    4312
XP_005246643                                                           17    4312
XP_005246642                                                           17    4312
XP_005246641                                                           17    4312
XP_005246640                                                           17    4312
XP_005246638                                                           17    4312
XP_005246639                                                           17    4312
XP_005246636                                                           17    4312
XP_005246637                                                           17    4312
XP_006712603                                                           17    4312
NP_001008844                                                           15    9605
NP_004406                                                              15    9605
NP_067047                                                              15    9605
NP_065689                                                              15    9605
XP_006717280                                                           15    9605
XP_006717279                                                           15    9605
XP_006717278                                                           15    9605
XP_006717277                                                           15    9605
XP_006717276                                                           15    9605
XP_006717275                                                           15    9605
XP_006717274                                                           15    9605
XP_006717273                                                           15    9605
XP_006717272                                                           15    9605

>NP_444256
          Length = 1794

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 851 AEQMDFRAN 859



>NP_444254
          Length = 1845

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 851 AEQMDFRAN 859



>NP_444253
          Length = 1914

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 920 AEQMDFRAN 928



>NP_444255
          Length = 1863

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 920 AEQMDFRAN 928



>XP_005247551
          Length = 1855

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 861 AEQMDFRAN 869



>XP_005247550
          Length = 1856

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 930 AEQMDFRAN 938



>XP_006713712
          Length = 1923

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 930 AEQMDFRAN 938



>XP_005247549
          Length = 1924

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AKQMDFRAN 9
           A+QMDFRAN
Sbjct: 930 AEQMDFRAN 938



>NP_000095
          Length = 543

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           A Q DFRAN
Sbjct: 488 AHQCDFRAN 496



>NP_005957
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246644
          Length = 457

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 342 AKQMEFLCN 350



>XP_005246643
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 371 AKQMEFLCN 379



>XP_005246642
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246641
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246640
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246638
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246639
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246636
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_005246637
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>XP_006712603
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKQMDFRAN 9
           AKQM+F  N
Sbjct: 372 AKQMEFLCN 380



>NP_001008844
          Length = 2272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KQMDFR 7
           KQ+DFR
Sbjct: 872 KQIDFR 877



>NP_004406
          Length = 2871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KQMDFR 7
           KQ+DFR
Sbjct: 872 KQIDFR 877



>NP_067047
          Length = 2506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>NP_065689
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   MDFRAN 9
           MDFR N
Sbjct: 96  MDFRGN 101



>XP_006717280
          Length = 2221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717279
          Length = 2506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717278
          Length = 2566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717277
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717276
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717275
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717274
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717273
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



>XP_006717272
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KQMDFRAN 9
            K  DF+AN
Sbjct: 1215 KHRDFKAN 1222



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.125    0.345 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 416,051
Number of extensions: 466
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)