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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_722541                                                              17    3301
NP_001092140                                                           17    3301
NP_001092141                                                           17    3301
XP_006718478                                                           17    3301
XP_006718477                                                           17    3301
XP_006718476                                                           17    3301
XP_006718475                                                           17    3301
XP_005273764                                                           17    3301
XP_006718474                                                           17    3301
NP_660204                                                              16    5631
NP_001020276                                                           16    5631
NP_001258671                                                           16    5631
NP_001258670                                                           16    5631
NP_001883                                                              16    5631
NP_004870                                                              15    9605
NP_001277064                                                           15    9605
NP_660277                                                              15    9605
NP_001137455                                                           15    9605
NP_001137453                                                           15    9605
NP_001137454                                                           15    9605
NP_055318                                                              15    9605
NP_001243064                                                           15    9605
NP_001307                                                              15    9605
NP_009166                                                              15    9605
XP_005262281                                                           15    9605
XP_005262280                                                           15    9605
XP_005262279                                                           15    9605
XP_005262278                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AKQLFSILF 9
            AKQLFS LF
Sbjct: 2805 AKQLFSSLF 2813



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AKQLFSILF 9
            AKQLFS LF
Sbjct: 2805 AKQLFSSLF 2813



>NP_722541
          Length = 609

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 335 KQLFSIL 341



>NP_001092140
          Length = 609

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 335 KQLFSIL 341



>NP_001092141
          Length = 609

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 335 KQLFSIL 341



>XP_006718478
          Length = 464

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 190 KQLFSIL 196



>XP_006718477
          Length = 464

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 190 KQLFSIL 196



>XP_006718476
          Length = 609

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 335 KQLFSIL 341



>XP_006718475
          Length = 609

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 335 KQLFSIL 341



>XP_005273764
          Length = 668

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 394 KQLFSIL 400



>XP_006718474
          Length = 897

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   KQLFSIL 8
           KQLFSIL
Sbjct: 623 KQLFSIL 629



>NP_660204
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           +QLF ILF
Sbjct: 278 RQLFRILF 285



>NP_001020276
          Length = 365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           +QLF ILF
Sbjct: 306 RQLFRILF 313



>NP_001258671
          Length = 276

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           +QLF ILF
Sbjct: 217 RQLFRILF 224



>NP_001258670
          Length = 325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           +QLF ILF
Sbjct: 278 RQLFRILF 285



>NP_001883
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           +QLF ILF
Sbjct: 278 RQLFRILF 285



>NP_004870
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSILF 9
           LFSILF
Sbjct: 265 LFSILF 270



>NP_001277064
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSILF 9
           LFSILF
Sbjct: 251 LFSILF 256



>NP_660277
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>NP_001137455
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>NP_001137453
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>NP_001137454
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>NP_055318
          Length = 2785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    AKQLFSILF 9
            A Q+F +LF
Sbjct: 2483 AAQIFGLLF 2491



>NP_001243064
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           KQ+F +LF
Sbjct: 712 KQIFILLF 719



>NP_001307
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KQLFSILF 9
           KQ+F +LF
Sbjct: 768 KQIFILLF 775



>NP_009166
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AKQLFSILF 9
           A Q+F++LF
Sbjct: 105 AAQVFALLF 113



>XP_005262281
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>XP_005262280
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>XP_005262279
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



>XP_005262278
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KQLFSILF 9
          K +FS+LF
Sbjct: 11 KYIFSLLF 18



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.152    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 364,037
Number of extensions: 623
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)