Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              19     869
XP_005251512                                                           19     869
NP_008835                                                              17    3301
NP_001075109                                                           17    3301
NP_640339                                                              16    5631
XP_006717042                                                           16    5631
XP_005251797                                                           16    5631
NP_001136089                                                           16    7355
NP_001185970                                                           16    7355
NP_001185971                                                           16    7355
NP_001185969                                                           16    7355
NP_001136091                                                           16    7355
NP_006132                                                              16    7355
NP_001561                                                              16    7355
NP_060549                                                              16    7355
NP_001273086                                                           16    7355
NP_001136090                                                           16    7355
NP_001622                                                              16    7355
NP_071764                                                              16    7355
NP_037368                                                              15    9605
NP_001161093                                                           15    9605
NP_060855                                                              15    9605
NP_835227                                                              15    9605
NP_075048                                                              15    9605
NP_055839                                                              15    9605
>NP_004963
          Length = 1132

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AKNILLFRE 9
           AKNILL RE
Sbjct: 676 AKNILLIRE 684
>XP_005251512
          Length = 1132

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AKNILLFRE 9
           AKNILL RE
Sbjct: 676 AKNILLIRE 684
>NP_008835
          Length = 4128

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    AKNILLFRE 9
            +KNIL FR+
Sbjct: 3317 SKNILAFRD 3325
>NP_001075109
          Length = 4097

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    AKNILLFRE 9
            +KNIL FR+
Sbjct: 3317 SKNILAFRD 3325
>NP_640339
          Length = 516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KNILLFRE 9
           KN+ LFRE
Sbjct: 341 KNLELFRE 348
>XP_006717042
          Length = 290

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KNILLFRE 9
           KN+ LFRE
Sbjct: 115 KNLELFRE 122
>XP_005251797
          Length = 475

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KNILLFRE 9
           KN+ LFRE
Sbjct: 300 KNLELFRE 307
>NP_001136089
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_001185970
          Length = 682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 476 RNLLLFR 482
>NP_001185971
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 500 RNLLLFR 506
>NP_001185969
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 498 RNLLLFR 504
>NP_001136091
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 452 RNLLLFR 458
>NP_006132
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KNILLFRE 9
          KNIL+F E
Sbjct: 55 KNILVFGE 62
>NP_001561
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KNILLFRE 9
           +NILL++E
Sbjct: 607 ENILLYKE 614
>NP_060549
          Length = 995

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AKNILLFRE 9
           AKNI+ +RE
Sbjct: 729 AKNIIEWRE 737
>NP_001273086
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KNILLFRE 9
          KNIL+F E
Sbjct: 55 KNILVFGE 62
>NP_001136090
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_001622
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AKNILLF 7
          AKN++LF
Sbjct: 52 AKNLILF 58
>NP_071764
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KNILLFR 8
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_037368
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ILLFRE 9
           ILLFRE
Sbjct: 115 ILLFRE 120
>NP_001161093
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ILLFRE 9
          ILLFRE
Sbjct: 51 ILLFRE 56
>NP_060855
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AKNILLFR 8
           A+N LLFR
Sbjct: 183 ARNPLLFR 190
>NP_835227
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KNILLFRE 9
           +N+LLF++
Sbjct: 156 RNVLLFKK 163
>NP_075048
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ILLFRE 9
           ILLFRE
Sbjct: 794 ILLFRE 799
>NP_055839
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ILLFRE 9
           ILLFRE
Sbjct: 796 ILLFRE 801
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.148    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 402,809
Number of extensions: 570
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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