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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060547                                                              17    2528
XP_006716105                                                           17    2528
NP_001159607                                                           17    2528
NP_955781                                                              15    9605
NP_055543                                                              15    9605
NP_060714                                                              15    9605
XP_006723877                                                           15    9605

>NP_060547
          Length = 759

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AKLSTTKTK 9
           AKL TTK K
Sbjct: 424 AKLQTTKVK 432



>XP_006716105
          Length = 734

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AKLSTTKTK 9
           AKL TTK K
Sbjct: 424 AKLQTTKVK 432



>NP_001159607
          Length = 618

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AKLSTTKTK 9
           AKL TTK K
Sbjct: 283 AKLQTTKVK 291



>NP_955781
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KLSTTK 7
          KLSTTK
Sbjct: 75 KLSTTK 80



>NP_055543
          Length = 1322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AKLSTTKTK 9
          AKLS+ +TK
Sbjct: 10 AKLSSHRTK 18



>NP_060714
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KLSTTK 7
          KLSTTK
Sbjct: 75 KLSTTK 80



>XP_006723877
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KLSTTK 7
          KLSTTK
Sbjct: 75 KLSTTK 80



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288   0.0971    0.216 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 297,705
Number of extensions: 264
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)