Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     665
NP_060633                                                              17    3301
NP_919268                                                              17    3301
NP_066566                                                              17    4312
NP_004565                                                              17    4312
NP_536340                                                              17    4312
NP_536341                                                              17    4312
NP_001243711                                                           17    4312
NP_001185642                                                           17    4312
NP_001243751                                                           17    4312
XP_006722018                                                           17    4312
XP_006722017                                                           17    4312
XP_006722016                                                           17    4312
XP_006722015                                                           17    4312
XP_006722014                                                           17    4312
XP_006722013                                                           17    4312
XP_006722012                                                           17    4312
NP_001073953                                                           16    5631
NP_443087                                                              16    5631
NP_955452                                                              16    5631
XP_006714108                                                           16    5631
XP_005265813                                                           16    5631
XP_005265812                                                           16    5631
XP_005265811                                                           16    5631
XP_006714107                                                           16    5631
XP_005265809                                                           16    5631
XP_006713543                                                           16    5631
XP_006711061                                                           16    5631
XP_006711060                                                           16    5631
XP_005244868                                                           16    5631
NP_065773                                                              16    5631
NP_079330                                                              16    7355
XP_005250316                                                           16    7355
NP_036304                                                              16    7355
NP_001156971                                                           16    7355
NP_277041                                                              16    7355
NP_001231800                                                           15    9605
NP_001334                                                              15    9605
NP_001274433                                                           15    9605
NP_775899                                                              15    9605
XP_006716232                                                           15    9605
XP_006716231                                                           15    9605
XP_005246870                                                           15    9605
XP_005246869                                                           15    9605
NP_001123917                                                           15    9605
NP_001274432                                                           15    9605
NP_006742                                                              15    9605
>NP_005219
          Length = 1210

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AKLLGVEEK 9
           AKLLG EEK
Sbjct: 859 AKLLGAEEK 867
>NP_060633
          Length = 304

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LLGVEEK 9
          LLG+EEK
Sbjct: 15 LLGIEEK 21
>NP_919268
          Length = 657

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AKLLGVEEK 9
           AKL GVEE+
Sbjct: 280 AKLSGVEEE 288
>NP_066566
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  AKLLGVEE 8
          AKL+G++E
Sbjct: 44 AKLIGIDE 51
>NP_004565
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 174 KLLGAEER 181
>NP_536340
          Length = 274

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 155 KLLGAEER 162
>NP_536341
          Length = 459

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 155 KLLGAEER 162
>NP_001243711
          Length = 493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 189 KLLGAEER 196
>NP_001185642
          Length = 470

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 166 KLLGAEER 173
>NP_001243751
          Length = 331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 27 KLLGAEER 34
>XP_006722018
          Length = 331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 27 KLLGAEER 34
>XP_006722017
          Length = 331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 27 KLLGAEER 34
>XP_006722016
          Length = 331

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 27 KLLGAEER 34
>XP_006722015
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 56 KLLGAEER 63
>XP_006722014
          Length = 379

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 75 KLLGAEER 82
>XP_006722013
          Length = 384

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KLLGVEEK 9
          KLLG EE+
Sbjct: 80 KLLGAEER 87
>XP_006722012
          Length = 483

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLLGVEEK 9
           KLLG EE+
Sbjct: 179 KLLGAEER 186
>NP_001073953
          Length = 762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLLGVEE 8
           KL+GVEE
Sbjct: 550 KLVGVEE 556
>NP_443087
          Length = 368

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KLLGVEEK 9
          K++GVEE+
Sbjct: 91 KIMGVEEE 98
>NP_955452
          Length = 2590

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKLLGVEE 8
           A+L+GVEE
Sbjct: 684 AELVGVEE 691
>XP_006714108
          Length = 1119

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 507 LLGLEEK 513
>XP_005265813
          Length = 1143

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 531 LLGLEEK 537
>XP_005265812
          Length = 1151

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 539 LLGLEEK 545
>XP_005265811
          Length = 1175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 563 LLGLEEK 569
>XP_006714107
          Length = 1233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 621 LLGLEEK 627
>XP_005265809
          Length = 1233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 621 LLGLEEK 627
>XP_006713543
          Length = 2591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKLLGVEE 8
           A+L+GVEE
Sbjct: 685 AELVGVEE 692
>XP_006711061
          Length = 1553

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLLGVEE 8
           KL+GVEE
Sbjct: 464 KLVGVEE 470
>XP_006711060
          Length = 1595

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLLGVEE 8
           KL+GVEE
Sbjct: 506 KLVGVEE 512
>XP_005244868
          Length = 1639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLLGVEE 8
           KL+GVEE
Sbjct: 550 KLVGVEE 556
>NP_065773
          Length = 1233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLGVEEK 9
           LLG+EEK
Sbjct: 621 LLGLEEK 627
>NP_079330
          Length = 1222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KLLGVEE 8
          +LLGVEE
Sbjct: 81 QLLGVEE 87
>XP_005250316
          Length = 561

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGVEEK 9
          LGVEEK
Sbjct: 63 LGVEEK 68
>NP_036304
          Length = 618

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGVEEK 9
          LGVEEK
Sbjct: 63 LGVEEK 68
>NP_001156971
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGVEEK 9
          LGVEEK
Sbjct: 13 LGVEEK 18
>NP_277041
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGVEEK 9
          LGVEEK
Sbjct: 25 LGVEEK 30
>NP_001231800
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  AKLLGVEE 8
          AKL+G+++
Sbjct: 52 AKLIGIDD 59
>NP_001334
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  AKLLGVEE 8
          AKL+G+++
Sbjct: 52 AKLIGIDD 59
>NP_001274433
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KLLGVEEK 9
           +LLG+EE+
Sbjct: 872 RLLGLEEQ 879
>NP_775899
          Length = 4462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  AKLLGVEE 8
          +KL+G EE
Sbjct: 28 SKLIGAEE 35
>XP_006716232
          Length = 2753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KLLGVEE 8
            K+LG+EE
Sbjct: 1799 KILGMEE 1805
>XP_006716231
          Length = 2753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    KLLGVEE 8
            K+LG+EE
Sbjct: 1799 KILGMEE 1805
>XP_005246870
          Length = 1252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KLLGVEEK 9
            +LLG+EE+
Sbjct: 1025 RLLGLEEQ 1032
>XP_005246869
          Length = 1255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KLLGVEEK 9
            +LLG+EE+
Sbjct: 1025 RLLGLEEQ 1032
>NP_001123917
          Length = 1259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KLLGVEEK 9
            +LLG+EE+
Sbjct: 1025 RLLGLEEQ 1032
>NP_001274432
          Length = 1237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KLLGVEEK 9
            +LLG+EE+
Sbjct: 1025 RLLGLEEQ 1032
>NP_006742
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KLLGVEEK 9
            +LLG+EE+
Sbjct: 1025 RLLGLEEQ 1032
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.131    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 693,860
Number of extensions: 1233
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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