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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_112571                                                              16    5631
NP_001182317                                                           16    5631
NP_001269852                                                           15    9605
NP_060260                                                              15    9605
NP_001263190                                                           15    9605
NP_652759                                                              15    9605
NP_570613                                                              15    9605
NP_775808                                                              15    9605
XP_006717286                                                           15    9605
XP_006717285                                                           15    9605
XP_006717284                                                           15    9605
XP_006717283                                                           15    9605
XP_006717282                                                           15    9605
XP_005277669                                                           15    9605
XP_005277638                                                           15    9605
XP_005277637                                                           15    9605
XP_005277636                                                           15    9605
NP_001003795                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AKAEITLFS 9
            AKAE TLFS
Sbjct: 1122 AKAETTLFS 1130



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AKAEITLFS 9
           AKAE TLFS
Sbjct: 917 AKAETTLFS 925



>NP_112571
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AKAEITL 7
           AKAE+TL
Sbjct: 125 AKAEVTL 131



>NP_001182317
          Length = 998

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AKAEITL 7
           AKAE+TL
Sbjct: 125 AKAEVTL 131



>NP_001269852
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 475 AEVTLFA 481



>NP_060260
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 473 AEVTLFA 479



>NP_001263190
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 304 AEVTLFA 310



>NP_652759
          Length = 1198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 585 AEVTLFA 591



>NP_570613
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 304 AEVTLFA 310



>NP_775808
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 844 ESELTLFS 851



>XP_006717286
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 481 AEVTLFA 487



>XP_006717285
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 211 AEVTLFA 217



>XP_006717284
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 348 AEVTLFA 354



>XP_006717283
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 473 AEVTLFA 479



>XP_006717282
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AEITLFS 9
           AE+TLF+
Sbjct: 481 AEVTLFA 487



>XP_005277669
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 844 ESELTLFS 851



>XP_005277638
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 650 ESELTLFS 657



>XP_005277637
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 773 ESELTLFS 780



>XP_005277636
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 844 ESELTLFS 851



>NP_001003795
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KAEITLFS 9
           ++E+TLFS
Sbjct: 844 ESELTLFS 851



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.125    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,349
Number of extensions: 240
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)