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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              18    1135
NP_001108225                                                           17    2528
NP_001265067                                                           17    2528
NP_000109                                                              17    2528
NP_066964                                                              16    5631
XP_005246893                                                           16    5631

>NP_003290
          Length = 803

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ITLVLKEE 9
           ITLVLKEE
Sbjct: 247 ITLVLKEE 254



>NP_001108225
          Length = 658

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   AITLVLKEE 9
           A+TLVLK+E
Sbjct: 367 AMTLVLKKE 375



>NP_001265067
          Length = 476

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   AITLVLKEE 9
           A+TLVLK+E
Sbjct: 185 AMTLVLKKE 193



>NP_000109
          Length = 625

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   AITLVLKEE 9
           A+TLVLK+E
Sbjct: 367 AMTLVLKKE 375



>NP_066964
          Length = 732

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ITLVLKEE 9
           ITL+ KEE
Sbjct: 683 ITLITKEE 690



>XP_005246893
          Length = 827

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ITLVLKEE 9
           ITL+ KEE
Sbjct: 778 ITLITKEE 785



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.132    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 533,084
Number of extensions: 698
Number of successful extensions: 6
Number of sequences better than 10000.0: 6
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)