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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_078815                                                              16    5631
NP_919445                                                              16    5631
NP_001135973                                                           16    7355
NP_115731                                                              16    7355
NP_001230131                                                           16    7355
NP_036548                                                              16    7355
NP_002821                                                              15    9605
NP_005898                                                              15    9605
XP_005267043                                                           15    9605

>NP_078815
          Length = 402

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRNKRAQS 9
           +RN RAQS
Sbjct: 208 LRNARAQS 215



>NP_919445
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRNKRAQS 9
           +RN RAQS
Sbjct: 234 LRNARAQS 241



>NP_001135973
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IRNKRAQ 8
          +RN+RAQ
Sbjct: 90 LRNRRAQ 96



>NP_115731
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IRNKRAQ 8
          +RN+RAQ
Sbjct: 90 LRNRRAQ 96



>NP_001230131
          Length = 753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AIRNKRA 7
          A+RN+RA
Sbjct: 56 AVRNRRA 62



>NP_036548
          Length = 1105

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AIRNKRA 7
          A+RN+RA
Sbjct: 56 AVRNRRA 62



>NP_002821
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   IRNKRA 7
           +RNKRA
Sbjct: 837 VRNKRA 842



>NP_005898
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AIRNKRAQ 8
           AIR KRA+
Sbjct: 191 AIREKRAK 198



>XP_005267043
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AIRNKRAQ 8
           AIR KRA+
Sbjct: 274 AIREKRAK 281



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.116    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 342,263
Number of extensions: 207
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)