Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              18    1935
XP_005248577                                                           18    1935
XP_005248576                                                           18    1935
NP_001264671                                                           17    2528
NP_063938                                                              17    2528
XP_005264490                                                           17    2528
NP_005674                                                              16    5631
NP_690875                                                              16    5631
NP_997011                                                              16    5631
XP_005247009                                                           16    5631
NP_073751                                                              16    7355
NP_891549                                                              16    7355
NP_055529                                                              16    7355
NP_009149                                                              16    7355
XP_005258190                                                           16    7355
XP_005258189                                                           16    7355
XP_006722402                                                           16    7355
XP_006721055                                                           16    7355
XP_006721054                                                           16    7355
XP_006721053                                                           16    7355
XP_006721052                                                           16    7355
NP_001167579                                                           16    7355
NP_055213                                                              15    9605
XP_006713649                                                           15    9605
XP_005247417                                                           15    9605
>NP_005912
          Length = 1512

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AIQLVVEDT 9
           AIQL VEDT
Sbjct: 868 AIQLGVEDT 876
>XP_005248577
          Length = 1349

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AIQLVVEDT 9
           AIQL VEDT
Sbjct: 705 AIQLGVEDT 713
>XP_005248576
          Length = 1375

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AIQLVVEDT 9
           AIQL VEDT
Sbjct: 731 AIQLGVEDT 739
>NP_001264671
          Length = 437

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IQLVVEDT 9
          IQLV++DT
Sbjct: 86 IQLVIQDT 93
>NP_063938
          Length = 512

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IQLVVEDT 9
           IQLV++DT
Sbjct: 161 IQLVIQDT 168
>XP_005264490
          Length = 454

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IQLVVEDT 9
           IQLV++DT
Sbjct: 103 IQLVIQDT 110
>NP_005674
          Length = 319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IQLVVEDT 9
           +QLV++DT
Sbjct: 307 VQLVLQDT 314
>NP_690875
          Length = 511

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           IQLV EDT
Sbjct: 339 IQLVWEDT 346
>NP_997011
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           IQLV EDT
Sbjct: 339 IQLVWEDT 346
>XP_005247009
          Length = 319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IQLVVEDT 9
           +QLV++DT
Sbjct: 307 VQLVLQDT 314
>NP_073751
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           +QLV EDT
Sbjct: 219 LQLVEEDT 226
>NP_891549
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           +QL VEDT
Sbjct: 334 LQLSVEDT 341
>NP_055529
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AIQLVVED 8
            A+QL+ ED
Sbjct: 1109 ALQLIAED 1116
>NP_009149
          Length = 664

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IQLVVEDT 9
           +QL++EDT
Sbjct: 221 LQLLMEDT 228
>XP_005258190
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IQLVVEDT 9
          +QLV EDT
Sbjct: 72 LQLVEEDT 79
>XP_005258189
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           +QLV EDT
Sbjct: 219 LQLVEEDT 226
>XP_006722402
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IQLVVEDT 9
           +QLV EDT
Sbjct: 219 LQLVEEDT 226
>XP_006721055
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AIQLVVED 8
           A+QL+ ED
Sbjct: 320 ALQLIAED 327
>XP_006721054
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AIQLVVED 8
            A+QL+ ED
Sbjct: 1109 ALQLIAED 1116
>XP_006721053
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AIQLVVED 8
            A+QL+ ED
Sbjct: 1109 ALQLIAED 1116
>XP_006721052
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AIQLVVED 8
            A+QL+ ED
Sbjct: 1109 ALQLIAED 1116
>NP_001167579
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IQLVVED 8
           IQ++VED
Sbjct: 415 IQMIVED 421
>NP_055213
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IQLVVED 8
           IQL+V+D
Sbjct: 244 IQLIVQD 250
>XP_006713649
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IQLVVED 8
           IQL+V+D
Sbjct: 220 IQLIVQD 226
>XP_005247417
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IQLVVED 8
           IQL+V+D
Sbjct: 244 IQLIVQD 250
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.132    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,944
Number of extensions: 701
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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