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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275661                                                           16    5631
NP_060939                                                              16    5631
NP_001275662                                                           16    5631
XP_005257564                                                           16    5631
NP_699189                                                              16    7355
NP_937762                                                              15    9605
NP_001121190                                                           15    9605
NP_000218                                                              15    9605
NP_001121189                                                           15    9605

>NP_001275661
          Length = 1127

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IKKTAGVS 9
           IKK++GVS
Sbjct: 714 IKKSSGVS 721



>NP_060939
          Length = 1134

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IKKTAGVS 9
           IKK++GVS
Sbjct: 714 IKKSSGVS 721



>NP_001275662
          Length = 1127

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IKKTAGVS 9
           IKK++GVS
Sbjct: 714 IKKSSGVS 721



>XP_005257564
          Length = 1125

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IKKTAGVS 9
           IKK++GVS
Sbjct: 714 IKKSSGVS 721



>NP_699189
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KKTAGV 8
          KKTAGV
Sbjct: 92 KKTAGV 97



>NP_937762
          Length = 3333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    IKKTAGV 8
            +KKT+GV
Sbjct: 2741 LKKTSGV 2747



>NP_001121190
          Length = 1668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    IKKTAGV 8
            +KKT+GV
Sbjct: 1076 LKKTSGV 1082



>NP_000218
          Length = 1724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    IKKTAGV 8
            +KKT+GV
Sbjct: 1132 LKKTSGV 1138



>NP_001121189
          Length = 3277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    IKKTAGV 8
            +KKT+GV
Sbjct: 2685 LKKTSGV 2691



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.116    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 415,963
Number of extensions: 463
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)