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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              18    1135
NP_444254                                                              18    1135
NP_444253                                                              18    1135
NP_444255                                                              18    1135
XP_005247551                                                           18    1135
XP_005247550                                                           18    1135
XP_006713712                                                           18    1135
XP_005247549                                                           18    1135
NP_009041                                                              18    1482
XP_005268084                                                           18    1482
NP_004949                                                              17    4312
XP_005263496                                                           17    4312
XP_005263495                                                           17    4312

>NP_444256
          Length = 1794

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1634 AIGRLSSM 1641



>NP_444254
          Length = 1845

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1685 AIGRLSSM 1692



>NP_444253
          Length = 1914

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1754 AIGRLSSM 1761



>NP_444255
          Length = 1863

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1703 AIGRLSSM 1710



>XP_005247551
          Length = 1855

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1695 AIGRLSSM 1702



>XP_005247550
          Length = 1856

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1696 AIGRLSSM 1703



>XP_006713712
          Length = 1923

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1764 AIGRLSSM 1771



>XP_005247549
          Length = 1924

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AIGRLSSM 8
            AIGRLSSM
Sbjct: 1764 AIGRLSSM 1771



>NP_009041
          Length = 2627

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    AIGRLSSMV 9
            A+GRL SMV
Sbjct: 1858 AVGRLDSMV 1866



>XP_005268084
          Length = 2627

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    AIGRLSSMV 9
            A+GRL SMV
Sbjct: 1858 AVGRLDSMV 1866



>NP_004949
          Length = 2549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IGRLSSM 8
           +GRLSSM
Sbjct: 715 VGRLSSM 721



>XP_005263496
          Length = 2322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IGRLSSM 8
           +GRLSSM
Sbjct: 488 VGRLSSM 494



>XP_005263495
          Length = 2549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IGRLSSM 8
           +GRLSSM
Sbjct: 715 VGRLSSM 721



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.133    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 258,128
Number of extensions: 206
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)