Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_079141                                                              17    2528
XP_005253541                                                           17    2528
NP_001124396                                                           17    3301
NP_001010866                                                           17    3301
NP_036298                                                              17    3301
XP_005251496                                                           17    3301
XP_006710481                                                           17    3301
XP_006710480                                                           17    3301
NP_054890                                                              16    5631
NP_000278                                                              16    7355
NP_689549                                                              16    7355
NP_001138663                                                           16    7355
NP_002967                                                              16    7355
XP_005249237                                                           16    7355
XP_006713184                                                           16    7355
XP_006712745                                                           16    7355
XP_006712744                                                           16    7355
XP_006712743                                                           16    7355
NP_000830                                                              16    7355
NP_001129396                                                           16    7355
NP_000780                                                              15    9605
NP_060445                                                              15    9605
NP_001274750                                                           15    9605
NP_001274751                                                           15    9605
NP_001274752                                                           15    9605
NP_057652                                                              15    9605
NP_001186681                                                           15    9605
NP_001129524                                                           15    9605
NP_689572                                                              15    9605
NP_001186682                                                           15    9605
NP_071373                                                              15    9605
XP_006723073                                                           15    9605
XP_006723072                                                           15    9605
XP_005258564                                                           15    9605
XP_005258563                                                           15    9605
XP_005258562                                                           15    9605
XP_005250256                                                           15    9605
XP_005266015                                                           15    9605
NP_659802                                                              15    9605
>NP_079141
          Length = 305

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IFSTTRLC 9
           +FS+T+LC
Sbjct: 101 VFSSTQLC 108
>XP_005253541
          Length = 289

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IFSTTRLC 9
           +FS+T+LC
Sbjct: 101 VFSSTQLC 108
>NP_001124396
          Length = 666

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           FSTT LC
Sbjct: 357 FSTTSLC 363
>NP_001010866
          Length = 392

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           FSTT LC
Sbjct: 357 FSTTSLC 363
>NP_036298
          Length = 956

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   STTRLC 9
           STTRLC
Sbjct: 175 STTRLC 180
>XP_005251496
          Length = 653

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   STTRLC 9
           STTRLC
Sbjct: 175 STTRLC 180
>XP_006710481
          Length = 542

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           FSTT LC
Sbjct: 357 FSTTSLC 363
>XP_006710480
          Length = 642

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           FSTT LC
Sbjct: 357 FSTTSLC 363
>NP_054890
          Length = 101

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FSTTRLC 9
          FST R+C
Sbjct: 55 FSTCRIC 61
>NP_000278
          Length = 980

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AIFSTTRLC 9
           AIFS  R C
Sbjct: 513 AIFSRARRC 521
>NP_689549
          Length = 1066

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FSTT+ C
Sbjct: 194 LFSTTQNC 201
>NP_001138663
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           F+TT LC
Sbjct: 605 FTTTSLC 611
>NP_002967
          Length = 1682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    IFSTTRLC 9
            IFS T LC
Sbjct: 1323 IFSITGLC 1330
>XP_005249237
          Length = 892

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AIFSTTRLC 9
           AIFS  R C
Sbjct: 425 AIFSRARRC 433
>XP_006713184
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STTRLC 9
           +TTRLC
Sbjct: 402 NTTRLC 407
>XP_006712745
          Length = 1682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    IFSTTRLC 9
            IFS T LC
Sbjct: 1323 IFSITGLC 1330
>XP_006712744
          Length = 1682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    IFSTTRLC 9
            IFS T LC
Sbjct: 1323 IFSITGLC 1330
>XP_006712743
          Length = 1682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    IFSTTRLC 9
            IFS T LC
Sbjct: 1323 IFSITGLC 1330
>NP_000830
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STTRLC 9
           +TTRLC
Sbjct: 402 NTTRLC 407
>NP_001129396
          Length = 1590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FSTT+ C
Sbjct: 119 LFSTTKDC 126
>NP_000780
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           I+ST ++C
Sbjct: 150 IYSTAKVC 157
>NP_060445
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FS T++C
Sbjct: 488 LFSLTQIC 495
>NP_001274750
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FS T++C
Sbjct: 374 LFSLTQIC 381
>NP_001274751
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FS T++C
Sbjct: 374 LFSLTQIC 381
>NP_001274752
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFSTTRLC 9
           +FS T++C
Sbjct: 374 LFSLTQIC 381
>NP_057652
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AIFSTT 6
           AIFSTT
Sbjct: 233 AIFSTT 238
>NP_001186681
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>NP_001129524
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 299 IFNTTRI 305
>NP_689572
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>NP_001186682
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>NP_071373
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           F+TT LC
Sbjct: 538 FATTYLC 544
>XP_006723073
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>XP_006723072
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 181 IFNTTRI 187
>XP_005258564
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>XP_005258563
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>XP_005258562
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IFSTTRL 8
           IF+TTR+
Sbjct: 310 IFNTTRI 316
>XP_005250256
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           F+TT LC
Sbjct: 520 FTTTYLC 526
>XP_005266015
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           F+TT LC
Sbjct: 538 FATTYLC 544
>NP_659802
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSTTRLC 9
           F+TT LC
Sbjct: 520 FTTTYLC 526
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.136    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,167
Number of extensions: 525
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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