Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     390
NP_000170                                                              20     390
NP_001268422                                                           20     390
NP_001268423                                                           20     390
XP_005264328                                                           20     390
NP_000746                                                              16    5631
NP_109592                                                              16    5631
NP_002839                                                              16    5631
NP_001244292                                                           16    5631
XP_005251766                                                           16    5631
XP_005251765                                                           16    5631
XP_005251764                                                           16    5631
XP_005251763                                                           16    5631
NP_002199                                                              16    7355
NP_940876                                                              16    7355
NP_001263653                                                           16    7355
NP_620142                                                              16    7355
NP_001263651                                                           16    7355
NP_001263652                                                           16    7355
XP_005252935                                                           16    7355
XP_006718201                                                           16    7355
NP_006218                                                              15    9605
NP_872617                                                              15    9605
NP_001229849                                                           15    9605
NP_001127870                                                           15    9605
NP_003362                                                              15    9605
NP_001229850                                                           15    9605
XP_005260496                                                           15    9605
XP_005272270                                                           15    9605
XP_006717340                                                           15    9605

>NP_001268421
          Length = 1230

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AIEDLMLVP 9
           AIEDLM+VP
Sbjct: 664 AIEDLMVVP 672



>NP_000170
          Length = 1360

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AIEDLMLVP 9
           AIEDLM+VP
Sbjct: 794 AIEDLMVVP 802



>NP_001268422
          Length = 1058

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AIEDLMLVP 9
           AIEDLM+VP
Sbjct: 492 AIEDLMVVP 500



>NP_001268423
          Length = 1058

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AIEDLMLVP 9
           AIEDLM+VP
Sbjct: 492 AIEDLMVVP 500



>XP_005264328
          Length = 1261

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AIEDLMLVP 9
           AIEDLM+VP
Sbjct: 695 AIEDLMVVP 703



>NP_000746
          Length = 626

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 527 AIEDLVSMP 535



>NP_109592
          Length = 1216

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IEDLMLVP 9
           I+DLML P
Sbjct: 533 IKDLMLYP 540



>NP_002839
          Length = 1188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IEDLMLVP 9
           I+DLML P
Sbjct: 533 IKDLMLYP 540



>NP_001244292
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 506 AIEDLVSMP 514



>XP_005251766
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 506 AIEDLVSMP 514



>XP_005251765
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 506 AIEDLVSMP 514



>XP_005251764
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 523 AIEDLVSMP 531



>XP_005251763
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AIEDLMLVP 9
           AIEDL+ +P
Sbjct: 528 AIEDLVSMP 536



>NP_002199
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 763 EDLLLMP 769



>NP_940876
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEDLMLVP 9
           +ED ML+P
Sbjct: 246 VEDRMLLP 253



>NP_001263653
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 119 EDLLLLP 125



>NP_620142
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 119 EDLLLLP 125



>NP_001263651
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 119 EDLLLLP 125



>NP_001263652
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 119 EDLLLLP 125



>XP_005252935
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEDLMLVP 9
           +ED ML+P
Sbjct: 246 VEDRMLLP 253



>XP_006718201
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EDLMLVP 9
           EDL+L+P
Sbjct: 119 EDLLLLP 125



>NP_006218
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AIEDLMLVP 9
           A+E L L+P
Sbjct: 401 ALESLALIP 409



>NP_872617
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AIEDLMLVP 9
           A+E L L+P
Sbjct: 349 ALESLALIP 357



>NP_001229849
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AIEDLMLVP 9
           A+E L L+P
Sbjct: 306 ALESLALIP 314



>NP_001127870
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IEDLMLVP 9
           ++DL++VP
Sbjct: 325 LQDLLVVP 332



>NP_003362
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IEDLMLVP 9
           ++DL++VP
Sbjct: 320 LQDLLVVP 327



>NP_001229850
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AIEDLMLVP 9
           A+E L L+P
Sbjct: 313 ALESLALIP 321



>XP_005260496
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AIEDLMLVP 9
           A+E L L+P
Sbjct: 421 ALESLALIP 429



>XP_005272270
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IEDLMLVP 9
           ++DL++VP
Sbjct: 320 LQDLLVVP 327



>XP_006717340
          Length = 857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IEDLMLVP 9
           ++DL++VP
Sbjct: 320 LQDLLVVP 327



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.146    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,019
Number of extensions: 706
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)