Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001098992                                                           18    1135
NP_001193556                                                           18    1135
NP_001193555                                                           18    1135
XP_006710016                                                           18    1135
XP_005275770                                                           18    1135
NP_001182163                                                           17    2528
NP_065091                                                              17    2528
NP_001278262                                                           17    4312
NP_004614                                                              17    4312
NP_001012339                                                           17    4312
NP_919259                                                              17    4312
XP_005276418                                                           17    4312
XP_005272298                                                           17    4312
XP_005248307                                                           17    4312
XP_005248306                                                           17    4312
XP_005276092                                                           17    4312
NP_055855                                                              16    5631
NP_001129175                                                           16    5631
NP_038465                                                              16    5631
NP_150091                                                              16    5631
NP_001121689                                                           16    5631
NP_066967                                                              16    5631
XP_005251415                                                           16    5631
XP_005251414                                                           16    5631
XP_005251413                                                           16    5631
XP_005251412                                                           16    5631
XP_006712425                                                           16    5631
XP_006712424                                                           16    5631
NP_002325                                                              16    7355
NP_036565                                                              16    7355
NP_071926                                                              16    7355
XP_006718294                                                           16    7355
XP_005252980                                                           16    7355
XP_005268557                                                           16    7355
XP_006714855                                                           16    7355
XP_006714854                                                           16    7355
XP_005268555                                                           16    7355
XP_005268554                                                           16    7355
XP_005246485                                                           16    7355
NP_001068567                                                           15    9605
NP_003717                                                              15    9605
NP_006398                                                              15    9605
NP_009119                                                              15    9605
NP_001269220                                                           15    9605
NP_001269222                                                           15    9605
XP_005257812                                                           15    9605
NP_001116848                                                           15    9605
NP_803875                                                              15    9605
>NP_001098992
          Length = 452

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +LRG
Sbjct: 220 AHRREILRG 228
>NP_001193556
          Length = 452

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +LRG
Sbjct: 220 AHRREILRG 228
>NP_001193555
          Length = 452

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +LRG
Sbjct: 220 AHRREILRG 228
>XP_006710016
          Length = 452

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +LRG
Sbjct: 220 AHRREILRG 228
>XP_005275770
          Length = 452

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +LRG
Sbjct: 220 AHRREILRG 228
>NP_001182163
          Length = 452

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AHRKAVLRG 9
           AHR  +LRG
Sbjct: 220 AHRMEILRG 228
>NP_065091
          Length = 452

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AHRKAVLRG 9
           AHR  +LRG
Sbjct: 220 AHRMEILRG 228
>NP_001278262
          Length = 288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HRKAVLRG 9
           H KA++RG
Sbjct: 150 HLKAIIRG 157
>NP_004614
          Length = 387

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HRKAVLRG 9
           H KA++RG
Sbjct: 150 HLKAIIRG 157
>NP_001012339
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  HRKAVLRG 9
          HR+A+L+G
Sbjct: 68 HREALLKG 75
>NP_919259
          Length = 576

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  HRKAVLRG 9
          HR+A+L+G
Sbjct: 68 HREALLKG 75
>XP_005276418
          Length = 193

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR A LRG
Sbjct: 13 HRPATLRG 20
>XP_005272298
          Length = 193

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR A LRG
Sbjct: 13 HRPATLRG 20
>XP_005248307
          Length = 466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  HRKAVLRG 9
          HR+A+L+G
Sbjct: 68 HREALLKG 75
>XP_005248306
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  HRKAVLRG 9
          HR+A+L+G
Sbjct: 68 HREALLKG 75
>XP_005276092
          Length = 193

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR A LRG
Sbjct: 13 HRPATLRG 20
>NP_055855
          Length = 2098

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AHRKAVLR 8
            +H KAVLR
Sbjct: 1992 SHSKAVLR 1999
>NP_001129175
          Length = 840

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HRKAVLR 8
           HRK +LR
Sbjct: 275 HRKVILR 281
>NP_038465
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HRKAVLR 8
           HRK +LR
Sbjct: 294 HRKVILR 300
>NP_150091
          Length = 530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>NP_001121689
          Length = 530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>NP_066967
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>XP_005251415
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>XP_005251414
          Length = 331

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>XP_005251413
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>XP_005251412
          Length = 351

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AHRKAVLRG 9
           AHR+ +L+G
Sbjct: 297 AHRRNMLKG 305
>XP_006712425
          Length = 1349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AHRKAVLR 8
            +H KAVLR
Sbjct: 1243 SHSKAVLR 1250
>XP_006712424
          Length = 1376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AHRKAVLR 8
            +H KAVLR
Sbjct: 1270 SHSKAVLR 1277
>NP_002325
          Length = 1905

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AHRKAVL 7
           +HRKAV+
Sbjct: 630 SHRKAVI 636
>NP_036565
          Length = 1304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AHRKAVLR 8
            AH+KA+ R
Sbjct: 1068 AHKKAIRR 1075
>NP_071926
          Length = 1544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_006718294
          Length = 1808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AHRKAVL 7
           +HRKAV+
Sbjct: 630 SHRKAVI 636
>XP_005252980
          Length = 1809

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AHRKAVL 7
           +HRKAV+
Sbjct: 630 SHRKAVI 636
>XP_005268557
          Length = 934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_006714855
          Length = 1473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_006714854
          Length = 1486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_005268555
          Length = 1524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_005268554
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HRKAVLR 8
          HRK +LR
Sbjct: 54 HRKRILR 60
>XP_005246485
          Length = 1158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AHRKAVLR 8
           AH+KA+ R
Sbjct: 922 AHKKAIRR 929
>NP_001068567
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR  +LRG
Sbjct: 37 HRDHILRG 44
>NP_003717
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR  +LRG
Sbjct: 37 HRDHILRG 44
>NP_006398
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AHRKAVLR 8
          AH K VLR
Sbjct: 84 AHNKDVLR 91
>NP_009119
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AHRKAVLRG 9
           AHR + +RG
Sbjct: 650 AHRLSTVRG 658
>NP_001269220
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AHRKAVLRG 9
           AHR + +RG
Sbjct: 667 AHRLSTVRG 675
>NP_001269222
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AHRKAVLRG 9
           AHR + +RG
Sbjct: 562 AHRLSTVRG 570
>XP_005257812
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  HRKAVLRG 9
          HR  +LRG
Sbjct: 37 HRDHILRG 44
>NP_001116848
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HRKAVLRG 9
           H K++L+G
Sbjct: 185 HMKSILKG 192
>NP_803875
          Length = 4243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    HRKAVLRG 9
            H KAVL G
Sbjct: 3849 HNKAVLVG 3856
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.137    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 479,113
Number of extensions: 727
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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