Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_071370                                                              17    2528
XP_006716147                                                           17    2528
XP_006716146                                                           17    2528
XP_006716145                                                           17    2528
NP_002169                                                              17    4312
NP_060555                                                              16    5631
NP_060695                                                              16    5631
NP_001265202                                                           16    5631
NP_599025                                                              16    5631
NP_001035544                                                           16    5631
NP_001268662                                                           16    5631
NP_075062                                                              16    5631
NP_872633                                                              16    5631
NP_872634                                                              16    5631
NP_872631                                                              16    5631
NP_005218                                                              16    5631
NP_001268661                                                           16    5631
NP_005039                                                              16    5631
XP_005251560                                                           16    5631
XP_005250608                                                           16    5631
XP_006716161                                                           16    5631
XP_005250607                                                           16    5631
XP_005250606                                                           16    5631
XP_006711503                                                           16    5631
NP_001956                                                              16    5631
NP_602298                                                              16    5631
NP_872632                                                              16    5631
NP_061178                                                              16    5631
NP_002438                                                              16    7355
NP_001231866                                                           16    7355
NP_001129687                                                           16    7355
NP_476432                                                              16    7355
NP_002283                                                              16    7355
XP_005265227                                                           16    7355
XP_005265184                                                           16    7355
XP_006711087                                                           16    7355
XP_006711086                                                           16    7355
NP_476431                                                              16    7355
XP_006710702                                                           15    9605
XP_005270828                                                           15    9605
>NP_071370
          Length = 608

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           GQCLRA
Sbjct: 576 GQCLRA 581
>XP_006716147
          Length = 608

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           GQCLRA
Sbjct: 576 GQCLRA 581
>XP_006716146
          Length = 608

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           GQCLRA
Sbjct: 576 GQCLRA 581
>XP_006716145
          Length = 608

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           GQCLRA
Sbjct: 576 GQCLRA 581
>NP_002169
          Length = 240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GQCLRARA 9
           G+CL ARA
Sbjct: 102 GRCLPARA 109
>NP_060555
          Length = 1041

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           G+CLRA
Sbjct: 714 GECLRA 719
>NP_060695
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           +GQCLR
Sbjct: 295 SGQCLR 300
>NP_001265202
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 677 AGECLR 682
>NP_599025
          Length = 758

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 61 QCSRARA 67
>NP_001035544
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 40 QCSRARA 46
>NP_001268662
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 61 QCSRARA 67
>NP_075062
          Length = 759

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 61 QCSRARA 67
>NP_872633
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 677 AGECLR 682
>NP_872634
          Length = 699

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 688 AGECLR 693
>NP_872631
          Length = 207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           +GQCLR
Sbjct: 141 SGQCLR 146
>NP_005218
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           +GQCLR
Sbjct: 141 SGQCLR 146
>NP_001268661
          Length = 723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 61 QCSRARA 67
>NP_005039
          Length = 550

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  GQCLRARA 9
          G CL ARA
Sbjct: 17 GSCLLARA 24
>XP_005251560
          Length = 462

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           +GQCLR
Sbjct: 244 SGQCLR 249
>XP_005250608
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 708 AGECLR 713
>XP_006716161
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 708 AGECLR 713
>XP_005250607
          Length = 730

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 719 AGECLR 724
>XP_005250606
          Length = 767

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           AG+CLR
Sbjct: 756 AGECLR 761
>XP_006711503
          Length = 860

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           G+CLRA
Sbjct: 533 GECLRA 538
>NP_001956
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  GQCLRARA 9
          G+C RARA
Sbjct: 41 GRCGRARA 48
>NP_602298
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QCLRARA 9
          QC RARA
Sbjct: 61 QCSRARA 67
>NP_872632
          Length = 193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AGQCLR 6
           +GQCLR
Sbjct: 141 SGQCLR 146
>NP_061178
          Length = 669

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QCLRARA 9
           QCL ARA
Sbjct: 640 QCLAARA 646
>NP_002438
          Length = 1400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           G+CLRA
Sbjct: 534 GRCLRA 539
>NP_001231866
          Length = 1351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           G+CLRA
Sbjct: 534 GRCLRA 539
>NP_001129687
          Length = 228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGQCLRARA 9
           +G C RAR+
Sbjct: 159 SGSCRRARS 167
>NP_476432
          Length = 220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGQCLRARA 9
           +G C RAR+
Sbjct: 151 SGSCRRARS 159
>NP_002283
          Length = 1798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2    GQCLR 6
            GQCLR
Sbjct: 1005 GQCLR 1009
>XP_005265227
          Length = 1401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GQCLRA 7
           G+CLRA
Sbjct: 534 GRCLRA 539
>XP_005265184
          Length = 1798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2    GQCLR 6
            GQCLR
Sbjct: 1005 GQCLR 1009
>XP_006711087
          Length = 237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGQCLRARA 9
           +G C RAR+
Sbjct: 168 SGSCRRARS 176
>XP_006711086
          Length = 237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGQCLRARA 9
           +G C RAR+
Sbjct: 168 SGSCRRARS 176
>NP_476431
          Length = 228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGQCLRARA 9
           +G C RAR+
Sbjct: 159 SGSCRRARS 167
>XP_006710702
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  QCLRAR 8
          +CLRAR
Sbjct: 61 RCLRAR 66
>XP_005270828
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AGQCLRAR 8
           AG+CL  R
Sbjct: 242 AGECLALR 249
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.132    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 597,213
Number of extensions: 627
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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