Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     509
NP_858045                                                              20     509
NP_006525                                                              20     509
NP_001167559                                                           20     509
NP_056303                                                              16    5631
NP_072094                                                              16    5631
NP_848645                                                              16    7355
NP_000262                                                              16    7355
NP_542402                                                              16    7355
NP_065829                                                              16    7355
NP_997465                                                              16    7355
NP_008988                                                              16    7355
NP_006725                                                              16    7355
NP_001005413                                                           16    7355
NP_127490                                                              16    7355
NP_001008395                                                           16    7355
XP_005259257                                                           16    7355
XP_005259256                                                           16    7355
XP_006722542                                                           16    7355
XP_005258336                                                           16    7355
XP_005258335                                                           16    7355
XP_005258334                                                           16    7355
XP_005269520                                                           16    7355
XP_005267014                                                           16    7355
XP_005245183                                                           16    7355
XP_006711352                                                           16    7355
XP_005245182                                                           16    7355
NP_003361                                                              16    7355
NP_775764                                                              15    9605
NP_663619                                                              15    9605
XP_006720233                                                           15    9605
XP_005267806                                                           15    9605
XP_006720232                                                           15    9605
XP_006720231                                                           15    9605
XP_005264418                                                           15    9605
NP_057706                                                              15    9605
NP_057421                                                              15    9605
>NP_001167558
          Length = 1423

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AGIMEPMNS 9
           AG MEPMNS
Sbjct: 927 AGRMEPMNS 935
>NP_858045
          Length = 1424

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AGIMEPMNS 9
           AG MEPMNS
Sbjct: 927 AGRMEPMNS 935
>NP_006525
          Length = 1420

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AGIMEPMNS 9
           AG MEPMNS
Sbjct: 927 AGRMEPMNS 935
>NP_001167559
          Length = 1415

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AGIMEPMNS 9
           AG MEPMNS
Sbjct: 922 AGRMEPMNS 930
>NP_056303
          Length = 385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGIMEPMN 8
           AG++EP N
Sbjct: 301 AGVVEPEN 308
>NP_072094
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGIMEPMN 8
           AG++EP N
Sbjct: 277 AGVVEPEN 284
>NP_848645
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGIMEPMNS 9
           AG+ +PM S
Sbjct: 128 AGLKDPMKS 136
>NP_000262
          Length = 1278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MEPMNS 9
           MEPMN+
Sbjct: 217 MEPMNN 222
>NP_542402
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  MEPMNS 9
          MEP+NS
Sbjct: 55 MEPLNS 60
>NP_065829
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GIMEPMN 8
           GI+EPM+
Sbjct: 785 GIIEPMD 791
>NP_997465
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  GIMEPMNS 9
          G+M P NS
Sbjct: 84 GLMRPANS 91
>NP_008988
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AGIMEPM 7
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_006725
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GIMEPM 7
           GIMEP+
Sbjct: 454 GIMEPL 459
>NP_001005413
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AGIMEPM 7
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_127490
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AGIMEPM 7
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_001008395
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   IMEPMN 8
           I+EPMN
Sbjct: 282 ILEPMN 287
>XP_005259257
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GIMEPMNS 9
           G+ME MN+
Sbjct: 262 GLMEEMNA 269
>XP_005259256
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GIMEPMNS 9
           G+ME MN+
Sbjct: 261 GLMEEMNA 268
>XP_006722542
          Length = 1273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MEPMNS 9
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258336
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MEPMNS 9
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258335
          Length = 1295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MEPMNS 9
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005258334
          Length = 1303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MEPMNS 9
           MEPMN+
Sbjct: 217 MEPMNN 222
>XP_005269520
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AGIMEPM 7
          AGI+EP+
Sbjct: 20 AGILEPV 26
>XP_005267014
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GIMEPM 7
           GIMEP+
Sbjct: 454 GIMEPL 459
>XP_005245183
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGIMEPMNS 9
           AG+ +PM S
Sbjct: 127 AGLKDPMKS 135
>XP_006711352
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGIMEPMNS 9
           AG+ +PM S
Sbjct: 127 AGLKDPMKS 135
>XP_005245182
          Length = 313

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AGIMEPMNS 9
           AG+ +PM S
Sbjct: 139 AGLKDPMKS 147
>NP_003361
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GIMEPMNS 9
           G+ME MN+
Sbjct: 262 GLMEEMNA 269
>NP_775764
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AGIMEPMN 8
           AG+M+P++
Sbjct: 335 AGLMDPVD 342
>NP_663619
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  AGIMEPMN 8
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
>XP_006720233
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GIMEPMN 8
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_005267806
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GIMEPMN 8
           G+ME MN
Sbjct: 272 GLMEEMN 278
>XP_006720232
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GIMEPMN 8
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_006720231
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GIMEPMN 8
           G+ME MN
Sbjct: 309 GLMEEMN 315
>XP_005264418
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  AGIMEPMN 8
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
>NP_057706
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  AGIMEPMN 8
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
>NP_057421
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GIMEPMN 8
           G+ME MN
Sbjct: 268 GLMEEMN 274
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.124    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 612,903
Number of extensions: 984
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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