Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_208382                                                              18    1482
NP_062552                                                              17    2528
NP_001156806                                                           17    2528
NP_620135                                                              17    2528
NP_001156807                                                           17    2528
NP_003314                                                              17    4312
XP_006723412                                                           17    4312
XP_006723411                                                           17    4312
XP_006723410                                                           17    4312
XP_006723409                                                           17    4312
XP_006723408                                                           17    4312
NP_001243224                                                           16    5631
NP_005230                                                              16    5631
XP_005260992                                                           16    5631
NP_671725                                                              16    7355
NP_757379                                                              16    7355
NP_001242953                                                           16    7355
NP_067049                                                              16    7355
NP_001242954                                                           16    7355
NP_004432                                                              16    7355
XP_006710418                                                           16    7355
NP_001278086                                                           15    9605
NP_004422                                                              15    9605
NP_001181876                                                           15    9605
NP_937887                                                              15    9605
NP_937884                                                              15    9605
NP_004944                                                              15    9605
NP_001181875                                                           15    9605
NP_937885                                                              15    9605
NP_886553                                                              15    9605
NP_001231293                                                           15    9605
NP_660350                                                              15    9605
NP_001269870                                                           15    9605
NP_001073948                                                           15    9605
NP_001120926                                                           15    9605
NP_001120925                                                           15    9605
NP_001185731                                                           15    9605
NP_001185730                                                           15    9605
NP_004394                                                              15    9605
NP_003751                                                              15    9605
NP_001171164                                                           15    9605
XP_005261469                                                           15    9605
XP_006717758                                                           15    9605
XP_005248625                                                           15    9605
XP_006714732                                                           15    9605
XP_005245809                                                           15    9605
XP_005245808                                                           15    9605
XP_006709999                                                           15    9605
NP_055107                                                              15    9605
>NP_208382
          Length = 648

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AGFEKKQRT 9
           AGFEK +RT
Sbjct: 282 AGFEKLRRT 290
>NP_062552
          Length = 689

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AGFEKKQR 8
           AGF+K+QR
Sbjct: 404 AGFKKQQR 411
>NP_001156806
          Length = 752

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GFEKKQRT 9
           G EKKQRT
Sbjct: 458 GDEKKQRT 465
>NP_620135
          Length = 730

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GFEKKQRT 9
           G EKKQRT
Sbjct: 436 GDEKKQRT 443
>NP_001156807
          Length = 730

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GFEKKQRT 9
           G EKKQRT
Sbjct: 436 GDEKKQRT 443
>NP_003314
          Length = 520

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 32 FEKKQR 37
>XP_006723412
          Length = 512

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 46 FEKKQR 51
>XP_006723411
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 32 FEKKQR 37
>XP_006723410
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 32 FEKKQR 37
>XP_006723409
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 46 FEKKQR 51
>XP_006723408
          Length = 545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FEKKQR 8
          FEKKQR
Sbjct: 46 FEKKQR 51
>NP_001243224
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AGFEKKQR 8
           +GF+K+QR
Sbjct: 228 SGFKKEQR 235
>NP_005230
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  AGFEKKQR 8
          +GF+K+QR
Sbjct: 88 SGFKKEQR 95
>XP_005260992
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  AGFEKKQR 8
          +GF+K+QR
Sbjct: 88 SGFKKEQR 95
>NP_671725
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AGFEKKQRT 9
           A F KKQR+
Sbjct: 124 AKFRKKQRS 132
>NP_757379
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AGFEKKQRT 9
           A F KKQR+
Sbjct: 119 AKFRKKQRS 127
>NP_001242953
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AGFEKKQRT 9
          AG+ KKQR+
Sbjct: 41 AGWLKKQRS 49
>NP_067049
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AGFEKKQRT 9
          AG+ KKQR+
Sbjct: 41 AGWLKKQRS 49
>NP_001242954
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AGFEKKQRT 9
          AG+ KKQR+
Sbjct: 47 AGWLKKQRS 55
>NP_004432
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGFEKKQR 8
           AG+ +KQR
Sbjct: 655 AGYSEKQR 662
>XP_006710418
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AGFEKKQRT 9
           A F KKQR+
Sbjct: 119 AKFRKKQRS 127
>NP_001278086
          Length = 1559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 821 FEKKQK 826
>NP_004422
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGFEKKQR 8
           AG+ +KQR
Sbjct: 650 AGYTEKQR 657
>NP_001181876
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 868 FEKKQK 873
>NP_937887
          Length = 1512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 774 FEKKQK 779
>NP_937884
          Length = 1599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 861 FEKKQK 866
>NP_004944
          Length = 1404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 666 FEKKQK 671
>NP_001181875
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 868 FEKKQK 873
>NP_937885
          Length = 1435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 697 FEKKQK 702
>NP_886553
          Length = 1600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 862 FEKKQK 867
>NP_001231293
          Length = 1392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GFEKKQ 7
           GFEK+Q
Sbjct: 151 GFEKEQ 156
>NP_660350
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FEKKQRT 9
          FE K+RT
Sbjct: 90 FESKERT 96
>NP_001269870
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FEKKQRT 9
          FE K+RT
Sbjct: 90 FESKERT 96
>NP_001073948
          Length = 1731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GFEKKQ 7
           GFEK+Q
Sbjct: 464 GFEKEQ 469
>NP_001120926
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GFEKKQR 8
          GFE K+R
Sbjct: 79 GFENKKR 85
>NP_001120925
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GFEKKQR 8
           GFE K+R
Sbjct: 243 GFENKKR 249
>NP_001185731
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 861 FEKKQK 866
>NP_001185730
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 891 FEKKQK 896
>NP_004394
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GFEKKQR 8
           GFE K+R
Sbjct: 243 GFENKKR 249
>NP_003751
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FEKKQR 8
           FEKKQ+
Sbjct: 855 FEKKQK 860
>NP_001171164
          Length = 1705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GFEKKQ 7
           GFEK+Q
Sbjct: 464 GFEKEQ 469
>XP_005261469
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  AGFEKKQR 8
          A FE+K+R
Sbjct: 55 AAFEQKER 62
>XP_006717758
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FEKKQRT 9
          FE K+RT
Sbjct: 90 FESKERT 96
>XP_005248625
          Length = 1418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GFEKKQ 7
           GFEK+Q
Sbjct: 151 GFEKEQ 156
>XP_006714732
          Length = 1633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GFEKKQ 7
           GFEK+Q
Sbjct: 366 GFEKEQ 371
>XP_005245809
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGFEKKQR 8
           AG+ +KQR
Sbjct: 596 AGYTEKQR 603
>XP_005245808
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AGFEKKQR 8
           AG+ +KQR
Sbjct: 639 AGYTEKQR 646
>XP_006709999
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  GFEKKQR 8
          GF+++QR
Sbjct: 16 GFDRRQR 22
>NP_055107
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  AGFEKKQR 8
          A FE+K+R
Sbjct: 55 AAFEQKER 62
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.126    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 734,864
Number of extensions: 1178
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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