Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_079303                                                              18    1482
XP_005259334                                                           18    1482
XP_005259333                                                           18    1482
NP_057331                                                              17    4312
NP_001091681                                                           17    4312
NP_001091683                                                           17    4312
NP_001091682                                                           17    4312
NP_000852                                                              17    4312
NP_002654                                                              16    5631
NP_001230037                                                           16    5631
XP_005256754                                                           16    5631
XP_005256753                                                           16    5631
XP_006721611                                                           16    5631
XP_006721610                                                           16    5631
NP_003985                                                              16    7355
NP_000890                                                              16    7355
NP_001230175                                                           16    7355
NP_005622                                                              16    7355
NP_996806                                                              16    7355
NP_071751                                                              16    7355
NP_001139761                                                           16    7355
XP_005271167                                                           16    7355
NP_000531                                                              15    9605
NP_001036188                                                           15    9605
NP_000113                                                              15    9605
NP_001269158                                                           15    9605
NP_001269155                                                           15    9605
NP_001269156                                                           15    9605
NP_001269154                                                           15    9605
NP_001269157                                                           15    9605
NP_954646                                                              15    9605
NP_620132                                                              15    9605
NP_001166983                                                           15    9605
NP_065789                                                              15    9605
NP_872434                                                              15    9605
NP_001073949                                                           15    9605
XP_006723382                                                           15    9605
XP_006723381                                                           15    9605
XP_006723380                                                           15    9605
XP_006717381                                                           15    9605
XP_005252359                                                           15    9605
XP_006715063                                                           15    9605
XP_006715062                                                           15    9605
XP_006713686                                                           15    9605
XP_006713685                                                           15    9605
XP_006713684                                                           15    9605
XP_006713683                                                           15    9605
XP_006713682                                                           15    9605
XP_006713681                                                           15    9605
XP_006713680                                                           15    9605
XP_005247488                                                           15    9605
XP_005263675                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFRDYVAVS 9
            AFRDYVAV+
Sbjct: 4073 AFRDYVAVA 4081
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFRDYVAVS 9
            AFRDYVAV+
Sbjct: 4042 AFRDYVAVA 4050
>NP_079303
          Length = 792

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AFRDYVAVS 9
           AFRDY A+S
Sbjct: 548 AFRDYSALS 556
>XP_005259334
          Length = 702

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AFRDYVAVS 9
           AFRDY A+S
Sbjct: 458 AFRDYSALS 466
>XP_005259333
          Length = 792

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AFRDYVAVS 9
           AFRDY A+S
Sbjct: 548 AFRDYSALS 556
>NP_057331
          Length = 1241

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FRDYVA 7
           FRDY+A
Sbjct: 354 FRDYIA 359
>NP_001091681
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RDYVAVS 9
           RDYVAV+
Sbjct: 319 RDYVAVN 325
>NP_001091683
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RDYVAVS 9
           RDYVAV+
Sbjct: 319 RDYVAVN 325
>NP_001091682
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RDYVAVS 9
           RDYVAV+
Sbjct: 319 RDYVAVN 325
>NP_000852
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RDYVAVS 9
           RDYVAV+
Sbjct: 319 RDYVAVN 325
>NP_002654
          Length = 933

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 728 AWRDYISI 735
>NP_001230037
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 728 AWRDYISI 735
>XP_005256754
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 308 AWRDYISI 315
>XP_005256753
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 343 AWRDYISI 350
>XP_006721611
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 585 AWRDYISI 592
>XP_006721610
          Length = 933

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   AFRDYVAV 8
           A+RDY+++
Sbjct: 728 AWRDYISI 735
>NP_003985
          Length = 245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AFRDYVAVS 9
           AF+D+V  S
Sbjct: 150 AFKDFVVAS 158
>NP_000890
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AFRDYVAVS 9
           AF+D+V  S
Sbjct: 150 AFKDFVVAS 158
>NP_001230175
          Length = 804

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRDYVAV 8
           FRDY A+
Sbjct: 609 FRDYSAI 615
>NP_005622
          Length = 787

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   AFRDYV 6
           +FRDYV
Sbjct: 483 SFRDYV 488
>NP_996806
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRDYVAV 8
           FRDY A+
Sbjct: 265 FRDYAAM 271
>NP_071751
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRDYVAV 8
           FRDY A+
Sbjct: 609 FRDYSAI 615
>NP_001139761
          Length = 697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRDYVAV 8
           FRDY A+
Sbjct: 609 FRDYSAI 615
>XP_005271167
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FRDYVAV 8
           FRDY A+
Sbjct: 273 FRDYSAI 279
>NP_000531
          Length = 5038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AFRDYV 6
            AF+DYV
Sbjct: 4075 AFQDYV 4080
>NP_001036188
          Length = 5033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AFRDYV 6
            AF+DYV
Sbjct: 4070 AFQDYV 4075
>NP_000113
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FRDYVAV 8
           +R+YVA+
Sbjct: 519 YREYVAI 525
>NP_001269158
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FRDYV 6
           FRDYV
Sbjct: 104 FRDYV 108
>NP_001269155
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FRDYV 6
           FRDYV
Sbjct: 224 FRDYV 228
>NP_001269156
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FRDYV 6
           FRDYV
Sbjct: 182 FRDYV 186
>NP_001269154
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FRDYV 6
           FRDYV
Sbjct: 224 FRDYV 228
>NP_001269157
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FRDYV 6
           FRDYV
Sbjct: 182 FRDYV 186
>NP_954646
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FRDYVAVS 9
          FR+Y+A S
Sbjct: 15 FRNYLAAS 22
>NP_620132
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FRDYVAVS 9
          FR+Y+A S
Sbjct: 15 FRNYLAAS 22
>NP_001166983
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FRDYVAVS 9
          FR+Y+A S
Sbjct: 15 FRNYLAAS 22
>NP_065789
          Length = 1771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    AFRDYVA 7
            A+R+Y+A
Sbjct: 1386 AYREYIA 1392
>NP_872434
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>NP_001073949
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FRDYVA 7
           F+DY+A
Sbjct: 203 FKDYIA 208
>XP_006723382
          Length = 5027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AFRDYV 6
            AF+DYV
Sbjct: 4064 AFQDYV 4069
>XP_006723381
          Length = 5031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AFRDYV 6
            AF+DYV
Sbjct: 4068 AFQDYV 4073
>XP_006723380
          Length = 5032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AFRDYV 6
            AF+DYV
Sbjct: 4069 AFQDYV 4074
>XP_006717381
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FRDYVAVS 9
          FR+Y+A S
Sbjct: 36 FRNYLAAS 43
>XP_005252359
          Length = 283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FRDYVAVS 9
          FR+Y+A S
Sbjct: 36 FRNYLAAS 43
>XP_006715063
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FRDYVA 7
           F+DY+A
Sbjct: 203 FKDYIA 208
>XP_006715062
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FRDYVA 7
           F+DY+A
Sbjct: 171 FKDYIA 176
>XP_006713686
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713685
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713684
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713683
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713682
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713681
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_006713680
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_005247488
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   AFRDY 5
           AFRDY
Sbjct: 126 AFRDY 130
>XP_005263675
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   FRDYVAV 8
           +R+YVA+
Sbjct: 455 YREYVAI 461
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.137    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 469,688
Number of extensions: 481
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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