Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1482
XP_006715292                                                           18    1482
NP_039253                                                              17    4312
XP_005273544                                                           17    4312
XP_005273543                                                           17    4312
XP_005273542                                                           17    4312
XP_006721993                                                           16    5631
NP_057085                                                              16    5631
NP_001019831                                                           16    7355
NP_009049                                                              16    7355
NP_003938                                                              16    7355
NP_003250                                                              16    7355
XP_006714558                                                           16    7355
XP_006713880                                                           16    7355
XP_006713879                                                           16    7355
XP_006713878                                                           16    7355
XP_006713877                                                           16    7355
XP_006713876                                                           16    7355
XP_006713875                                                           16    7355
XP_006713874                                                           16    7355
XP_006713873                                                           16    7355
NP_001957                                                              15    9605
NP_001245258                                                           15    9605
NP_001245260                                                           15    9605
NP_060587                                                              15    9605
NP_683692                                                              15    9605
NP_001159887                                                           15    9605
NP_001245259                                                           15    9605
NP_818932                                                              15    9605
NP_036288                                                              15    9605
XP_005260382                                                           15    9605
XP_005260381                                                           15    9605
XP_006723804                                                           15    9605
XP_005260380                                                           15    9605
XP_006723803                                                           15    9605
XP_005260379                                                           15    9605
XP_005260378                                                           15    9605
XP_005260377                                                           15    9605
XP_006723802                                                           15    9605
XP_005260376                                                           15    9605
XP_006723801                                                           15    9605
XP_006723800                                                           15    9605
XP_006723799                                                           15    9605
XP_006723798                                                           15    9605
XP_005270021                                                           15    9605
XP_005270020                                                           15    9605
XP_005270019                                                           15    9605
XP_005248208                                                           15    9605
XP_005248207                                                           15    9605
XP_006714021                                                           15    9605
XP_006713588                                                           15    9605
XP_001718723                                                           15    9605
>NP_115927
          Length = 1321

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFLSTSTST 9
           A LSTSTST
Sbjct: 317 ALLSTSTST 325
>XP_006715292
          Length = 759

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFLSTSTST 9
           A LSTSTST
Sbjct: 304 ALLSTSTST 312
>NP_039253
          Length = 296

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTSTST
Sbjct: 222 LSTSTST 228
>XP_005273544
          Length = 692

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTSTST
Sbjct: 222 LSTSTST 228
>XP_005273543
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTSTST
Sbjct: 222 LSTSTST 228
>XP_005273542
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTSTST
Sbjct: 222 LSTSTST 228
>XP_006721993
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLSTSTS 8
           F+STSTS
Sbjct: 241 FISTSTS 247
>NP_057085
          Length = 556

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLSTSTS 8
           F+STSTS
Sbjct: 224 FISTSTS 230
>NP_001019831
          Length = 2986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1153 YLSTHTST 1160
>NP_009049
          Length = 3097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1161 YLSTHTST 1168
>NP_003938
          Length = 1663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1153 YLSTHTST 1160
>NP_003250
          Length = 924

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AFLSTSTST 9
           AF++T+T+T
Sbjct: 281 AFVATATAT 289
>XP_006714558
          Length = 2612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FLSTSTST 9
           +LST TST
Sbjct: 676 YLSTHTST 683
>XP_006713880
          Length = 1530

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1155 YLSTHTST 1162
>XP_006713879
          Length = 1656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1146 YLSTHTST 1153
>XP_006713878
          Length = 1665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1155 YLSTHTST 1162
>XP_006713877
          Length = 2956

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1155 YLSTHTST 1162
>XP_006713876
          Length = 2966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1133 YLSTHTST 1140
>XP_006713875
          Length = 2975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1142 YLSTHTST 1149
>XP_006713874
          Length = 2979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1146 YLSTHTST 1153
>XP_006713873
          Length = 2988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLSTSTST 9
            +LST TST
Sbjct: 1155 YLSTHTST 1162
>NP_001957
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AFLSTSTS 8
           AFL T+TS
Sbjct: 404 AFLCTNTS 411
>NP_001245258
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 802 LSTSTTT 808
>NP_001245260
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 701 LSTSTTT 707
>NP_060587
          Length = 1224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AFLSTSTST 9
           +FLS +TST
Sbjct: 528 SFLSFATST 536
>NP_683692
          Length = 3051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AFLSTSTS 8
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>NP_001159887
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AFLSTSTS 8
           AFL T+TS
Sbjct: 308 AFLCTNTS 315
>NP_001245259
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 623 LSTSTTT 629
>NP_818932
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 701 LSTSTTT 707
>NP_036288
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 803 LSTSTTT 809
>XP_005260382
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 654 LSTSTTT 660
>XP_005260381
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 666 LSTSTTT 672
>XP_006723804
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 741 LSTSTTT 747
>XP_005260380
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 763 LSTSTTT 769
>XP_006723803
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 775 LSTSTTT 781
>XP_005260379
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 789 LSTSTTT 795
>XP_005260378
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 790 LSTSTTT 796
>XP_005260377
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 791 LSTSTTT 797
>XP_006723802
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 802 LSTSTTT 808
>XP_005260376
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 802 LSTSTTT 808
>XP_006723801
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 803 LSTSTTT 809
>XP_006723800
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 803 LSTSTTT 809
>XP_006723799
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 803 LSTSTTT 809
>XP_006723798
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSTSTST 9
           LSTST+T
Sbjct: 838 LSTSTTT 844
>XP_005270021
          Length = 753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AFLSTSTST 9
          +FLS +TST
Sbjct: 57 SFLSFATST 65
>XP_005270020
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AFLSTSTST 9
           +FLS +TST
Sbjct: 269 SFLSFATST 277
>XP_005270019
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AFLSTSTST 9
           +FLS +TST
Sbjct: 269 SFLSFATST 277
>XP_005248208
          Length = 3097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AFLSTSTS 8
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>XP_005248207
          Length = 3098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AFLSTSTS 8
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>XP_006714021
          Length = 3112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AFLSTSTS 8
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>XP_006713588
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AFLSTSTS 8
           AFL T+TS
Sbjct: 196 AFLCTNTS 203
>XP_001718723
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LSTSTST 9
          LST+TST
Sbjct: 46 LSTATST 52
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299   0.0996    0.229 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 288,967
Number of extensions: 359
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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