Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_258411                                                              17    3301
XP_005257845                                                           17    3301
XP_005257844                                                           17    3301
NP_003050                                                              17    4312
XP_006714738                                                           17    4312
NP_004557                                                              16    5631
NP_001138915                                                           16    5631
NP_001269559                                                           16    5631
NP_001193665                                                           16    5631
NP_061327                                                              16    5631
NP_057227                                                              16    5631
XP_005252521                                                           16    5631
XP_005252520                                                           16    5631
NP_001128214                                                           16    7355
NP_055394                                                              16    7355
NP_001184113                                                           16    7355
NP_005500                                                              16    7355
NP_001277250                                                           16    7355
NP_115597                                                              16    7355
XP_006723946                                                           16    7355
XP_005260632                                                           16    7355
XP_005260631                                                           16    7355
XP_005260630                                                           16    7355
XP_006714623                                                           16    7355
XP_005271970                                                           16    7355
XP_005271969                                                           16    7355
XP_005271967                                                           16    7355
XP_005271966                                                           16    7355
NP_060250                                                              15    9605
NP_001035706                                                           15    9605
NP_078863                                                              15    9605
NP_001139627                                                           15    9605
NP_001139626                                                           15    9605
NP_689657                                                              15    9605
XP_005255000                                                           15    9605
XP_005254998                                                           15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605
XP_005271253                                                           15    9605
NP_787107                                                              15    9605
NP_663761                                                              15    9605
NP_001013665                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AFFLESIGM 9
            AFFLESI M
Sbjct: 1295 AFFLESIAM 1303
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AFFLESIGM 9
            AFFLESI M
Sbjct: 1295 AFFLESIAM 1303
>NP_258411
          Length = 638

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFFLES+
Sbjct: 612 AFFLESV 618
>XP_005257845
          Length = 400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFFLES+
Sbjct: 374 AFFLESV 380
>XP_005257844
          Length = 400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFFLES+
Sbjct: 374 AFFLESV 380
>NP_003050
          Length = 551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FFLESIGM 9
           FF ES+GM
Sbjct: 506 FFPESLGM 513
>XP_006714738
          Length = 375

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FFLESIGM 9
           FF ES+GM
Sbjct: 330 FFPESLGM 337
>NP_004557
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFF+ES+
Sbjct: 148 AFFIESV 154
>NP_001138915
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFF+ES+
Sbjct: 128 AFFIESV 134
>NP_001269559
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFF+ES+
Sbjct: 162 AFFIESV 168
>NP_001193665
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FFLESIG 8
          FFLES G
Sbjct: 25 FFLESAG 31
>NP_061327
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FFLESIG 8
          FFLES G
Sbjct: 25 FFLESAG 31
>NP_057227
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FFLESIG 8
          FFLES G
Sbjct: 25 FFLESAG 31
>XP_005252521
          Length = 462

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFF+ES+
Sbjct: 148 AFFIESV 154
>XP_005252520
          Length = 514

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AFFLESI 7
           AFF+ES+
Sbjct: 148 AFFIESV 154
>NP_001128214
          Length = 1094

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    FLESIGM 9
            FL+S+GM
Sbjct: 1052 FLQSLGM 1058
>NP_055394
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FFLESIGM 9
           FFLES+ +
Sbjct: 117 FFLESLAL 124
>NP_001184113
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FFLESIGM 9
           FFL +IG+
Sbjct: 117 FFLSAIGL 124
>NP_005500
          Length = 3027

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>NP_001277250
          Length = 3048

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>NP_115597
          Length = 2715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1308 FLEKVGM 1314
>XP_006723946
          Length = 1414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1308 FLEKVGM 1314
>XP_005260632
          Length = 2377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1308 FLEKVGM 1314
>XP_005260631
          Length = 2667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1260 FLEKVGM 1266
>XP_005260630
          Length = 2693

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1286 FLEKVGM 1292
>XP_006714623
          Length = 1625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271970
          Length = 2931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271969
          Length = 3020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FFLESI 7
          FFLESI
Sbjct: 41 FFLESI 46
>XP_005271967
          Length = 3065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271966
          Length = 3086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   FFLESI 7
           FFLESI
Sbjct: 107 FFLESI 112
>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1817 FLERVGM 1823
>NP_001035706
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FFLESIG 8
           FFL++IG
Sbjct: 497 FFLKNIG 503
>NP_078863
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFLESIGM 9
          FF ES+G+
Sbjct: 76 FFSESLGI 83
>NP_001139627
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFFLESIGM 9
           ++FL +IGM
Sbjct: 127 SYFLGAIGM 135
>NP_001139626
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFFLESIGM 9
           ++FL +IGM
Sbjct: 127 SYFLGAIGM 135
>NP_689657
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFFLESIGM 9
           ++FL +IGM
Sbjct: 127 SYFLGAIGM 135
>XP_005255000
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FFLESIG 8
           FFL++IG
Sbjct: 461 FFLKNIG 467
>XP_005254998
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FFLESIG 8
           FFL++IG
Sbjct: 497 FFLKNIG 503
>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FLESIGM 9
           FLE +GM
Sbjct: 732 FLERVGM 738
>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1012 FLERVGM 1018
>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1817 FLERVGM 1823
>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLESIGM 9
            FLE +GM
Sbjct: 1817 FLERVGM 1823
>XP_005271253
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FFLESIGM 9
          FF ES+G+
Sbjct: 3  FFSESLGI 10
>NP_787107
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FFLESIG 8
          FFL S+G
Sbjct: 34 FFLASVG 40
>NP_663761
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FFLESIGM 9
           FF +++GM
Sbjct: 108 FFFDALGM 115
>NP_001013665
          Length = 98

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  FFLESIG 8
          FF+ES G
Sbjct: 76 FFIESCG 82
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.151    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 365,238
Number of extensions: 471
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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