Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
NP_001177652                                                           17    3301
NP_001005374                                                           17    3301
NP_612370                                                              17    3301
NP_001005373                                                           17    3301
NP_002963                                                              17    3301
NP_006575                                                              17    3301
NP_001180459                                                           17    3301
NP_001180458                                                           17    3301
XP_005261992                                                           17    3301
XP_005261991                                                           17    3301
XP_005261989                                                           17    3301
XP_005261988                                                           17    3301
XP_006721705                                                           17    3301
XP_006721704                                                           17    3301
XP_006717379                                                           17    3301
NP_057427                                                              16    5631
NP_001273886                                                           15    9605
NP_149115                                                              15    9605
XP_006719734                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4312 AFEAAQVEA 4320
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4328 AFEAAQVEA 4336
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4329 AFEAAQVEA 4337
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4360 AFEAAQVEA 4368
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4384 AFEAAQVEA 4392
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4383 AFEAAQVEA 4391
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4383 AFEAAQVEA 4391
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4383 AFEAAQVEA 4391
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AFEAAQVKA 9
            AFEAAQV+A
Sbjct: 4384 AFEAAQVEA 4392
>NP_001177652
          Length = 696

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AFEAAQVK 8
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_001005374
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AFEAAQVK 8
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_612370
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AFEAAQVK 8
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_001005373
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AFEAAQVK 8
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_002963
          Length = 1893

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    FEAAQVKA 9
            FEA QVKA
Sbjct: 1380 FEARQVKA 1387
>NP_006575
          Length = 530

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FEAAQVKA 9
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>NP_001180459
          Length = 485

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FEAAQVKA 9
          FEAA++KA
Sbjct: 78 FEAAKIKA 85
>NP_001180458
          Length = 493

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FEAAQVKA 9
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>XP_005261992
          Length = 1867

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    FEAAQVKA 9
            FEA QVKA
Sbjct: 1354 FEARQVKA 1361
>XP_005261991
          Length = 1868

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    FEAAQVKA 9
            FEA QVKA
Sbjct: 1355 FEARQVKA 1362
>XP_005261989
          Length = 1891

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    FEAAQVKA 9
            FEA QVKA
Sbjct: 1378 FEARQVKA 1385
>XP_005261988
          Length = 1894

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    FEAAQVKA 9
            FEA QVKA
Sbjct: 1381 FEARQVKA 1388
>XP_006721705
          Length = 464

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FEAAQVKA 9
          FEAA++KA
Sbjct: 57 FEAAKIKA 64
>XP_006721704
          Length = 516

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FEAAQVKA 9
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>XP_006717379
          Length = 690

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AFEAAQVK 8
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_057427
          Length = 3114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AFEAAQVK 8
           AF+A+Q+K
Sbjct: 466 AFQASQIK 473
>NP_001273886
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AFEAAQVK 8
           AFE  QVK
Sbjct: 100 AFETLQVK 107
>NP_149115
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AFEAAQVK 8
           AFE  QVK
Sbjct: 243 AFETLQVK 250
>XP_006719734
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AFEAAQVK 8
           AFE  QVK
Sbjct: 243 AFETLQVK 250
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.114    0.272 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 220,754
Number of extensions: 197
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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