Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001398                                                              17    2528
NP_001549                                                              17    3301
NP_001399                                                              17    3301
XP_006718896                                                           17    3301
XP_006710468                                                           17    3301
XP_005270637                                                           17    3301
NP_060040                                                              17    4312
NP_061835                                                              17    4312
NP_055061                                                              17    4312
XP_006724446                                                           17    4312
NP_001154819                                                           16    5631
NP_001154817                                                           16    5631
NP_060693                                                              16    5631
NP_001154818                                                           16    5631
NP_001154816                                                           16    5631
NP_001034302                                                           16    5631
NP_060024                                                              16    5631
XP_005247602                                                           16    5631
NP_001171479                                                           16    7355
NP_001171480                                                           16    7355
NP_000062                                                              16    7355
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
XP_006724120                                                           16    7355
XP_006723989                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
XP_006711545                                                           16    7355
XP_006711544                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_005895                                                              15    9605
NP_001177750                                                           15    9605
NP_001091995                                                           15    9605
NP_065134                                                              15    9605
NP_060674                                                              15    9605
NP_775871                                                              15    9605
XP_005255896                                                           15    9605
XP_005255895                                                           15    9605
XP_005266401                                                           15    9605
XP_005266400                                                           15    9605
XP_005269094                                                           15    9605
NP_001073961                                                           15    9605
NP_001005289                                                           15    9605
NP_001273615                                                           15    9605
>NP_001398
          Length = 3312

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AEVTRTGC 8
            AEVT +GC
Sbjct: 2115 AEVTASGC 2122
>NP_001549
          Length = 578

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EVTRTGCL 9
           + T+TGCL
Sbjct: 456 KATKTGCL 463
>NP_001399
          Length = 2923

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            AEVT  GC
Sbjct: 1962 AEVTTNGC 1969
>XP_006718896
          Length = 558

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EVTRTGCL 9
           + T+TGCL
Sbjct: 436 KATKTGCL 443
>XP_006710468
          Length = 2916

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            AEVT  GC
Sbjct: 1962 AEVTTNGC 1969
>XP_005270637
          Length = 2921

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            AEVT  GC
Sbjct: 1962 AEVTTNGC 1969
>NP_060040
          Length = 1288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AEVTRTG 7
           AEVT+TG
Sbjct: 709 AEVTKTG 715
>NP_061835
          Length = 190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  AEVTRTG 7
          AE+TRTG
Sbjct: 49 AELTRTG 55
>NP_055061
          Length = 3014

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            AEVT  GC
Sbjct: 2041 AEVTTLGC 2048
>XP_006724446
          Length = 3019

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            AEVT  GC
Sbjct: 2041 AEVTTLGC 2048
>NP_001154819
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 433 TRTGC 437
>NP_001154817
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 524 TRTGC 528
>NP_060693
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 484 TRTGC 488
>NP_001154818
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 513 TRTGC 517
>NP_001154816
          Length = 664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 525 TRTGC 529
>NP_001034302
          Length = 425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   TRTGC 8
           TRTGC
Sbjct: 234 TRTGC 238
>NP_060024
          Length = 1801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AEVTRTGCL 9
           AEV  TGC+
Sbjct: 643 AEVIITGCV 651
>XP_005247602
          Length = 1665

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AEVTRTGCL 9
           AEV  TGC+
Sbjct: 507 AEVIITGCV 515
>NP_001171479
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AEVTRTGC 8
          AEV  TGC
Sbjct: 8  AEVGPTGC 15
>NP_001171480
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AEVTRTGC 8
          AEV  TGC
Sbjct: 8  AEVGPTGC 15
>NP_000062
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AEVTRTGC 8
          AEV  TGC
Sbjct: 8  AEVGPTGC 15
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 12547 ASVTKTSC 12554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 12211 SDVTKTSC 12218
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 12672 ASVTKTSC 12679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 12336 SDVTKTSC 12343
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 19971 ASVTKTSC 19978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 19635 SDVTKTSC 19642
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 19044 ASVTKTSC 19051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 18708 SDVTKTSC 18715
>XP_006724120
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AEVTRTGC 8
          AEV  TGC
Sbjct: 8  AEVGPTGC 15
>XP_006723989
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  AEVTRTGC 8
          AEV  TGC
Sbjct: 8  AEVGPTGC 15
>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            A VT+T C
Sbjct: 8284 ASVTKTSC 8291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    AEVTRTGC 8
            ++VT+T C
Sbjct: 7948 SDVTKTSC 7955
>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    AEVTRTGC 8
            A VT+T C
Sbjct: 9643 ASVTKTSC 9650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    AEVTRTGC 8
            ++VT+T C
Sbjct: 9307 SDVTKTSC 9314
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 19599 ASVTKTSC 19606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 19263 SDVTKTSC 19270
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 21105 ASVTKTSC 21112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 20769 SDVTKTSC 20776
>XP_006711545
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VTRTGC 8
           VTR GC
Sbjct: 186 VTRAGC 191
>XP_006711544
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VTRTGC 8
           VTR GC
Sbjct: 186 VTRAGC 191
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 21612 ASVTKTSC 21619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 21276 SDVTKTSC 21283
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1     AEVTRTGC 8
             A VT+T C
Sbjct: 12739 ASVTKTSC 12746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1     AEVTRTGC 8
             ++VT+T C
Sbjct: 12403 SDVTKTSC 12410
>NP_005895
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 121 TRTACL 126
>NP_001177750
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 121 TRTACL 126
>NP_001091995
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 584 TRTSCL 589
>NP_065134
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  AEVTRT 6
          AEVTRT
Sbjct: 17 AEVTRT 22
>NP_060674
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 583 TRTSCL 588
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    AEVTRT 6
            AEVTRT
Sbjct: 4298 AEVTRT 4303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    AEVTRT 6
            AEVTRT
Sbjct: 5397 AEVTRT 5402
>XP_005255896
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EVTRTGCL 9
           E T+T CL
Sbjct: 112 EETKTACL 119
>XP_005255895
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EVTRTGCL 9
           E T+T CL
Sbjct: 211 EETKTACL 218
>XP_005266401
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 536 TRTSCL 541
>XP_005266400
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 578 TRTSCL 583
>XP_005269094
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  AEVTRT 6
          AEVTRT
Sbjct: 17 AEVTRT 22
>NP_001073961
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EVTRTGCL 9
           E T+T CL
Sbjct: 211 EETKTACL 218
>NP_001005289
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EVTRTGCL 9
           E+T  GCL
Sbjct: 99  EITFPGCL 106
>NP_001273615
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TRTGCL 9
           TRT CL
Sbjct: 535 TRTSCL 540
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.132    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 482,620
Number of extensions: 528
Number of successful extensions: 60
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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