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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     299
NP_001138498                                                           16    5631
NP_079255                                                              16    5631
NP_038203                                                              16    5631
NP_789792                                                              16    5631
NP_002152                                                              16    5631
NP_057218                                                              16    5631
XP_005268373                                                           16    5631
XP_006718281                                                           16    5631
XP_006716561                                                           16    5631
XP_005250812                                                           16    5631
NP_056209                                                              16    5631
NP_001271200                                                           16    7355
NP_001128119                                                           16    7355
NP_006819                                                              16    7355
XP_006715385                                                           16    7355
XP_006713973                                                           16    7355
XP_006713971                                                           16    7355
NP_000375                                                              15    9605
NP_689961                                                              15    9605
NP_001136221                                                           15    9605
NP_065777                                                              15    9605
XP_006715958                                                           15    9605
XP_006715957                                                           15    9605
XP_006714724                                                           15    9605
XP_005248616                                                           15    9605

>NP_004110
          Length = 993

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AEEVMYQNV 9
           AEE MYQNV
Sbjct: 950 AEEAMYQNV 958



>NP_001138498
          Length = 2299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AEEVMYQNV 9
           A EV+Y+N+
Sbjct: 698 AYEVLYKNI 706



>NP_079255
          Length = 1123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  AEEVMYQNV 9
          A+E+M+Q++
Sbjct: 24 ADEIMHQDI 32



>NP_038203
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVMYQNV 9
           E+MYQN+
Sbjct: 778 ELMYQNL 784



>NP_789792
          Length = 1093

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EEVMYQ 7
           EE+MYQ
Sbjct: 245 EEIMYQ 250



>NP_002152
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVMYQNV 9
           E+MYQN+
Sbjct: 778 ELMYQNL 784



>NP_057218
          Length = 472

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  AEEVMYQNV 9
          A ++MYQN+
Sbjct: 87 AIQIMYQNL 95



>XP_005268373
          Length = 984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  AEEVMYQNV 9
          A+E+M+Q++
Sbjct: 24 ADEIMHQDI 32



>XP_006718281
          Length = 983

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EEVMYQ 7
           EE+MYQ
Sbjct: 135 EEIMYQ 140



>XP_006716561
          Length = 304

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  AEEVMYQNV 9
          A ++MYQN+
Sbjct: 87 AIQIMYQNL 95



>XP_005250812
          Length = 472

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  AEEVMYQNV 9
          A ++MYQN+
Sbjct: 87 AIQIMYQNL 95



>NP_056209
          Length = 573

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  AEEVMYQNV 9
          A++++Y+NV
Sbjct: 34 AQQIVYRNV 42



>NP_001271200
          Length = 731

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EEVMYQNV 9
           +EV Y+NV
Sbjct: 701 DEVCYENV 708



>NP_001128119
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 527 EEVLYDN 533



>NP_006819
          Length = 2202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EEVMYQNV 9
           +EV Y+NV
Sbjct: 701 DEVCYENV 708



>XP_006715385
          Length = 1866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EEVMYQNV 9
           +EV Y+NV
Sbjct: 365 DEVCYENV 372



>XP_006713973
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 548 EEVLYDN 554



>XP_006713971
          Length = 835

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 548 EEVLYDN 554



>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  AEEVMYQNV 9
          AEE M +NV
Sbjct: 27 AEEEMLENV 35



>NP_689961
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 823 EEVVYSN 829



>NP_001136221
          Length = 1029

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 990 EEVVYSN 996



>NP_065777
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  EEVMYQN 8
          EEV Y+N
Sbjct: 81 EEVTYEN 87



>XP_006715958
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 876 EEVVYSN 882



>XP_006715957
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   EEVMYQN 8
           EEV+Y N
Sbjct: 876 EEVVYSN 882



>XP_006714724
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  EEVMYQN 8
          EEV Y+N
Sbjct: 58 EEVTYEN 64



>XP_005248616
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  EEVMYQN 8
          EEV Y+N
Sbjct: 81 EEVTYEN 87



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.120    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 520,528
Number of extensions: 859
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)