Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_940890                                                              17    3301
NP_004746                                                              17    3301
XP_006725153                                                           17    3301
XP_006725152                                                           17    3301
XP_006725151                                                           17    3301
XP_006725150                                                           17    3301
XP_005250947                                                           17    3301
XP_005250946                                                           17    3301
XP_005250945                                                           17    3301
XP_005250944                                                           17    3301
NP_940978                                                              17    4312
XP_006710698                                                           17    4312
XP_005244806                                                           17    4312
XP_006710697                                                           17    4312
XP_006710696                                                           17    4312
NP_001157745                                                           16    5631
NP_001074010                                                           16    5631
NP_001026859                                                           16    5631
NP_001252519                                                           16    5631
NP_958831                                                              16    5631
NP_001252518                                                           16    5631
XP_005254311                                                           16    5631
XP_006720508                                                           16    5631
XP_006720507                                                           16    5631
XP_005254310                                                           16    5631
XP_005254309                                                           16    5631
XP_005254306                                                           16    5631
XP_005254305                                                           16    5631
XP_005254304                                                           16    5631
XP_005254303                                                           16    5631
XP_006720506                                                           16    5631
XP_005254302                                                           16    5631
XP_005254301                                                           16    5631
XP_006720505                                                           16    5631
XP_005254300                                                           16    5631
NP_001158145                                                           16    7355
NP_000287                                                              16    7355
NP_001009944                                                           16    7355
NP_079172                                                              16    7355
NP_001258791                                                           16    7355
NP_001159756                                                           16    7355
NP_006420                                                              16    7355
NP_001009570                                                           16    7355
NP_001159757                                                           16    7355
NP_001257711                                                           16    7355
NP_003192                                                              16    7355
NP_653267                                                              16    7355
NP_775787                                                              16    7355
NP_001136113                                                           16    7355
XP_006720958                                                           16    7355
XP_006720957                                                           16    7355
XP_005255427                                                           16    7355
XP_006720956                                                           16    7355
XP_006720955                                                           16    7355
XP_006720954                                                           16    7355
XP_006720953                                                           16    7355
XP_006720952                                                           16    7355
XP_006719805                                                           16    7355
XP_005266236                                                           16    7355
XP_005266235                                                           16    7355
XP_005266234                                                           16    7355
XP_005266233                                                           16    7355
XP_006719804                                                           16    7355
XP_005266232                                                           16    7355
XP_005266231                                                           16    7355
XP_005266230                                                           16    7355
XP_005266229                                                           16    7355
XP_005266228                                                           16    7355
XP_005253228                                                           16    7355
NP_109591                                                              15    9605
NP_001273685                                                           15    9605
NP_001805                                                              15    9605
NP_037499                                                              15    9605
XP_006715071                                                           15    9605
XP_005248216                                                           15    9605
XP_006714024                                                           15    9605
>NP_940890
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 821 DTLGRSGS 828
>NP_004746
          Length = 802

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D+LG SGA
Sbjct: 702 DILGSSGA 709
>XP_006725153
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 821 DTLGRSGS 828
>XP_006725152
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 821 DTLGRSGS 828
>XP_006725151
          Length = 1185

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 827 DTLGRSGS 834
>XP_006725150
          Length = 1198

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 840 DTLGRSGS 847
>XP_005250947
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 821 DTLGRSGS 828
>XP_005250946
          Length = 1179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 821 DTLGRSGS 828
>XP_005250945
          Length = 1185

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 827 DTLGRSGS 834
>XP_005250944
          Length = 1198

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D LGRSG+
Sbjct: 840 DTLGRSGS 847
>NP_940978
          Length = 2045

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           V+GRSGA
Sbjct: 369 VMGRSGA 375
>XP_006710698
          Length = 1907

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           V+GRSGA
Sbjct: 369 VMGRSGA 375
>XP_005244806
          Length = 2049

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           V+GRSGA
Sbjct: 369 VMGRSGA 375
>XP_006710697
          Length = 2053

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           V+GRSGA
Sbjct: 369 VMGRSGA 375
>XP_006710696
          Length = 2057

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           V+GRSGA
Sbjct: 369 VMGRSGA 375
>NP_001157745
          Length = 3065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2222 SDLLGKSG 2229
>NP_001074010
          Length = 2856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2013 SDLLGKSG 2020
>NP_001026859
          Length = 795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           DV+G SGA
Sbjct: 379 DVVGSSGA 386
>NP_001252519
          Length = 920

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D+LGRS A
Sbjct: 458 DILGRSPA 465
>NP_958831
          Length = 1013

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D+LGRS A
Sbjct: 551 DILGRSPA 558
>NP_001252518
          Length = 1032

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           D+LGRS A
Sbjct: 570 DILGRSPA 577
>XP_005254311
          Length = 1627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ADVLGRSG 8
           +D+LG+SG
Sbjct: 783 SDLLGKSG 790
>XP_006720508
          Length = 2658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 1814 SDLLGKSG 1821
>XP_006720507
          Length = 2763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 1919 SDLLGKSG 1926
>XP_005254310
          Length = 2906

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2062 SDLLGKSG 2069
>XP_005254309
          Length = 2956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2112 SDLLGKSG 2119
>XP_005254306
          Length = 3066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2222 SDLLGKSG 2229
>XP_005254305
          Length = 3114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_005254304
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_005254303
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_006720506
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_005254302
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_005254301
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_006720505
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>XP_005254300
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    ADVLGRSG 8
            +D+LG+SG
Sbjct: 2271 SDLLGKSG 2278
>NP_001158145
          Length = 255

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VLGRSGA 9
           VLGR GA
Sbjct: 181 VLGRGGA 187
>NP_000287
          Length = 4302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2450 VLGRSG 2455
>NP_001009944
          Length = 4303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2450 VLGRSG 2455
>NP_079172
          Length = 904

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VLGRSGA 9
           +LGRSG+
Sbjct: 609 ILGRSGS 615
>NP_001258791
          Length = 531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ADVLGRSG 8
           A +LGRSG
Sbjct: 523 AFILGRSG 530
>NP_001159756
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ADVLGR 6
           ADVLGR
Sbjct: 252 ADVLGR 257
>NP_006420
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ADVLGR 6
           ADVLGR
Sbjct: 339 ADVLGR 344
>NP_001009570
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ADVLGR 6
           ADVLGR
Sbjct: 135 ADVLGR 140
>NP_001159757
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ADVLGR 6
           ADVLGR
Sbjct: 295 ADVLGR 300
>NP_001257711
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGRSGA 9
          LGRSGA
Sbjct: 14 LGRSGA 19
>NP_003192
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LGRSGA 9
          LGRSGA
Sbjct: 14 LGRSGA 19
>NP_653267
          Length = 4753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    DVLGRS 7
            DVLGRS
Sbjct: 1641 DVLGRS 1646
>NP_775787
          Length = 520

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ADVLGRSG 8
           A +LGRSG
Sbjct: 512 AFILGRSG 519
>NP_001136113
          Length = 1045

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 810 LGRSGA 815
>XP_006720958
          Length = 2675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2487 VLGRSG 2492
>XP_006720957
          Length = 2688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2487 VLGRSG 2492
>XP_005255427
          Length = 3288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 1435 VLGRSG 1440
>XP_006720956
          Length = 3895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2042 VLGRSG 2047
>XP_006720955
          Length = 4279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2426 VLGRSG 2431
>XP_006720954
          Length = 4334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2487 VLGRSG 2492
>XP_006720953
          Length = 4339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2487 VLGRSG 2492
>XP_006720952
          Length = 4340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VLGRSG 8
            VLGRSG
Sbjct: 2487 VLGRSG 2492
>XP_006719805
          Length = 1009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 774 LGRSGA 779
>XP_005266236
          Length = 986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 751 LGRSGA 756
>XP_005266235
          Length = 1015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 780 LGRSGA 785
>XP_005266234
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 808 LGRSGA 813
>XP_005266233
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 809 LGRSGA 814
>XP_006719804
          Length = 1051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 816 LGRSGA 821
>XP_005266232
          Length = 1062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 827 LGRSGA 832
>XP_005266231
          Length = 1062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 827 LGRSGA 832
>XP_005266230
          Length = 1083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 848 LGRSGA 853
>XP_005266229
          Length = 1084

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 849 LGRSGA 854
>XP_005266228
          Length = 1085

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 850 LGRSGA 855
>XP_005253228
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGRSGA 9
           LGRSGA
Sbjct: 467 LGRSGA 472
>NP_109591
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ADVLGRSGA 9
           AD+ G SGA
Sbjct: 302 ADLSGMSGA 310
>NP_001273685
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ADVLGRS 7
           ADV+G+S
Sbjct: 542 ADVIGKS 548
>NP_001805
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DVLGRSGA 9
           DVLGR+ A
Sbjct: 128 DVLGRNWA 135
>NP_037499
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ADVLGRS 7
           ADV+G+S
Sbjct: 541 ADVIGKS 547
>XP_006715071
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ADVLGRSGA 9
           AD+ G SGA
Sbjct: 302 ADLSGMSGA 310
>XP_005248216
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ADVLGRS 7
           ADV+G+S
Sbjct: 174 ADVIGKS 180
>XP_006714024
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ADVLGRS 7
           ADV+G+S
Sbjct: 518 ADVIGKS 524
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.134    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 584,423
Number of extensions: 1356
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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