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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1935
NP_001087241                                                           18    1935
XP_006714101                                                           18    1935
XP_005265799                                                           18    1935
XP_005265798                                                           18    1935
XP_005265797                                                           18    1935
NP_078880                                                              16    7355
NP_001139547                                                           16    7355
NP_001139548                                                           16    7355

>NP_000213
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>NP_001087241
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>XP_006714101
          Length = 526

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>XP_005265799
          Length = 973

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>XP_005265798
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>XP_005265797
          Length = 977

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           A IMIKSVK
Sbjct: 168 AGIMIKSVK 176



>NP_078880
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           ADI+IKS +
Sbjct: 259 ADIVIKSTE 267



>NP_001139547
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           ADI+IKS +
Sbjct: 259 ADIVIKSTE 267



>NP_001139548
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ADIMIKSVK 9
           ADI+IKS +
Sbjct: 100 ADIVIKSTE 108



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.131    0.310 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 213,529
Number of extensions: 211
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)