Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     299
XP_006713721                                                           20     299
NP_037523                                                              17    3301
XP_006714661                                                           17    3301
XP_006714660                                                           17    3301
NP_008835                                                              16    5631
NP_001075109                                                           16    5631
NP_056081                                                              16    5631
NP_001247420                                                           16    5631
XP_006723821                                                           16    5631
XP_006723820                                                           16    5631
XP_006723819                                                           16    5631
NP_001367                                                              16    7355
NP_001609                                                              16    7355
NP_006023                                                              16    7355
NP_001267487                                                           16    7355
XP_006725598                                                           16    7355
XP_006726299                                                           16    7355
XP_005268273                                                           16    7355
XP_006719145                                                           16    7355
NP_076410                                                              16    7355
NP_004172                                                              15    9605
NP_694983                                                              15    9605
NP_001273567                                                           15    9605
NP_001257881                                                           15    9605
NP_001093112                                                           15    9605
NP_001258468                                                           15    9605
NP_060818                                                              15    9605
NP_055387                                                              15    9605
NP_005993                                                              15    9605
XP_006723716                                                           15    9605
XP_006723715                                                           15    9605
XP_006723714                                                           15    9605
XP_006723713                                                           15    9605
XP_006723712                                                           15    9605
XP_006723711                                                           15    9605
XP_006723710                                                           15    9605
XP_006723709                                                           15    9605
XP_005256104                                                           15    9605
XP_005256103                                                           15    9605
XP_005266572                                                           15    9605
XP_006719926                                                           15    9605
XP_005266571                                                           15    9605
XP_005266399                                                           15    9605
XP_006719861                                                           15    9605
XP_006719860                                                           15    9605
XP_006719859                                                           15    9605
XP_006719858                                                           15    9605
XP_005248822                                                           15    9605
XP_005248564                                                           15    9605
NP_005900                                                              15    9605
NP_078852                                                              15    9605
>NP_006209
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ALEYFMKQ 9
            ALEYFMKQ
Sbjct: 1035 ALEYFMKQ 1042
>XP_006713721
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ALEYFMKQ 9
            ALEYFMKQ
Sbjct: 1035 ALEYFMKQ 1042
>NP_037523
          Length = 866

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AALEYFMK 8
           A LEYF+K
Sbjct: 740 AGLEYFVK 747
>XP_006714661
          Length = 486

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AALEYFMK 8
           A LEYF+K
Sbjct: 360 AGLEYFVK 367
>XP_006714660
          Length = 774

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AALEYFMK 8
           A LEYF+K
Sbjct: 740 AGLEYFVK 747
>NP_008835
          Length = 4128

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AALEYFMKQ 9
           +ALE F+KQ
Sbjct: 314 SALESFLKQ 322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LEYFMKQ 9
           L+YFM+Q
Sbjct: 338 LQYFMEQ 344
>NP_001075109
          Length = 4097

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AALEYFMKQ 9
           +ALE F+KQ
Sbjct: 314 SALESFLKQ 322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LEYFMKQ 9
           L+YFM+Q
Sbjct: 338 LQYFMEQ 344
>NP_056081
          Length = 581

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ALEYFMK 8
           AL+YF+K
Sbjct: 260 ALDYFLK 266
>NP_001247420
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ALEYFMK 8
           AL+YF+K
Sbjct: 276 ALDYFLK 282
>XP_006723821
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ALEYFMK 8
          AL+YF+K
Sbjct: 13 ALDYFLK 19
>XP_006723820
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ALEYFMK 8
          AL+YF+K
Sbjct: 13 ALDYFLK 19
>XP_006723819
          Length = 614

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ALEYFMK 8
           AL+YF+K
Sbjct: 293 ALDYFLK 299
>NP_001367
          Length = 4646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ALEYFMKQ 9
            ALE F+KQ
Sbjct: 1458 ALEEFLKQ 1465
>NP_001609
          Length = 1014

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ALEYFMK 8
           A+E+FMK
Sbjct: 610 AIEHFMK 616
>NP_006023
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ALEYFMKQ 9
          ALEYF ++
Sbjct: 76 ALEYFFEE 83
>NP_001267487
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ALEYFMKQ 9
           ALEYF ++
Sbjct: 131 ALEYFFEE 138
>XP_006725598
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EYFMKQ 9
           EYF+KQ
Sbjct: 170 EYFIKQ 175
>XP_006726299
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EYFMKQ 9
           EYF+KQ
Sbjct: 170 EYFIKQ 175
>XP_005268273
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ALEYFMKQ 9
          ALEYF ++
Sbjct: 76 ALEYFFEE 83
>XP_006719145
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EYFMKQ 9
           EYF+KQ
Sbjct: 170 EYFIKQ 175
>NP_076410
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EYFMKQ 9
           EYF+KQ
Sbjct: 170 EYFIKQ 175
>NP_004172
          Length = 223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 80 YFMKQ 84
>NP_694983
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 190 LEYFIK 195
>NP_001273567
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 127 LEYFIK 132
>NP_001257881
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 49 YFMKQ 53
>NP_001093112
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ALEYFMKQ 9
           A++YFM++
Sbjct: 400 AIQYFMQK 407
>NP_001258468
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>NP_060818
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ALEYFMKQ 9
           A++YFM++
Sbjct: 400 AIQYFMQK 407
>NP_055387
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AALEYFMK 8
           AALEY  K
Sbjct: 131 AALEYISK 138
>NP_005993
          Length = 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 85 YFMKQ 89
>XP_006723716
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_006723715
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_006723714
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_006723713
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_006723712
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 236 ALHYFLK 242
>XP_006723711
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 302 ALHYFLK 308
>XP_006723710
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_006723709
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ALEYFMK 8
           AL YF+K
Sbjct: 321 ALHYFLK 327
>XP_005256104
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ALEYFMKQ 9
           A++YFM++
Sbjct: 400 AIQYFMQK 407
>XP_005256103
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ALEYFMKQ 9
           A++YFM++
Sbjct: 400 AIQYFMQK 407
>XP_005266572
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 49 YFMKQ 53
>XP_006719926
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 85 YFMKQ 89
>XP_005266571
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  YFMKQ 9
          YFMKQ
Sbjct: 85 YFMKQ 89
>XP_005266399
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AALEYFMK 8
           AALEY  K
Sbjct: 131 AALEYISK 138
>XP_006719861
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 111 LEYFIK 116
>XP_006719860
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 190 LEYFIK 195
>XP_006719859
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 190 LEYFIK 195
>XP_006719858
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEYFMK 8
           LEYF+K
Sbjct: 190 LEYFIK 195
>XP_005248822
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AALEYFM 7
           +ALEYF+
Sbjct: 279 SALEYFV 285
>XP_005248564
          Length = 2342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   LEYFMKQ 9
           ++YFM+Q
Sbjct: 308 MQYFMQQ 314
>NP_005900
          Length = 2468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   LEYFMKQ 9
           ++YFM+Q
Sbjct: 434 MQYFMQQ 440
>NP_078852
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AALEYFM 7
           +ALEYF+
Sbjct: 278 SALEYFV 284
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.132    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 416,226
Number of extensions: 541
Number of successful extensions: 54
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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