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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1935
XP_006713520                                                           17    4312
NP_001769                                                              16    5631
NP_001242959                                                           16    5631
XP_005245682                                                           16    5631
NP_001073861                                                           15    9605
NP_612354                                                              15    9605
XP_005257123                                                           15    9605
XP_006713882                                                           15    9605
XP_006713881                                                           15    9605

>NP_004295
          Length = 1620

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAIEILLMP 9
           AA EILLMP
Sbjct: 371 AAREILLMP 379



>XP_006713520
          Length = 285

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAIEILLMP 9
           AA E+L++P
Sbjct: 122 AAFEVLILP 130



>NP_001769
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  AIEILLMP 9
          A+E+LL+P
Sbjct: 11 ALELLLLP 18



>NP_001242959
          Length = 252

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  AIEILLMP 9
          A+E+LL+P
Sbjct: 11 ALELLLLP 18



>XP_005245682
          Length = 154

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  AIEILLMP 9
          A+E+LL+P
Sbjct: 11 ALELLLLP 18



>NP_001073861
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  AAIEILLMP 9
          AA E+LL P
Sbjct: 56 AAPEVLLQP 64



>NP_612354
          Length = 1242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIEILLMP 9
           A+EILL P
Sbjct: 265 ALEILLHP 272



>XP_005257123
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AAIEILLMP 9
           AA+ +LL P
Sbjct: 124 AAVSVLLAP 132



>XP_006713882
          Length = 1242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIEILLMP 9
           A+EILL P
Sbjct: 265 ALEILLHP 272



>XP_006713881
          Length = 1024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AIEILLMP 9
          A+EILL P
Sbjct: 46 ALEILLHP 53



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.146    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,822
Number of extensions: 604
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)