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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              18    1482
XP_005265800                                                           18    1482
XP_006714103                                                           18    1482
XP_006714102                                                           18    1482
NP_783864                                                              17    3301
XP_005262701                                                           17    3301
NP_004786                                                              17    4312
NP_795362                                                              16    7355
NP_005190                                                              15    9605
NP_001007562                                                           15    9605
XP_005258573                                                           15    9605
XP_005258572                                                           15    9605

>NP_006197
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAHNVLLAQ 9
           AA NVLLAQ
Sbjct: 820 AARNVLLAQ 828



>XP_005265800
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAHNVLLAQ 9
           AA NVLLAQ
Sbjct: 820 AARNVLLAQ 828



>XP_006714103
          Length = 1111

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAHNVLLAQ 9
           AA NVLLAQ
Sbjct: 842 AARNVLLAQ 850



>XP_006714102
          Length = 1114

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAHNVLLAQ 9
           AA NVLLAQ
Sbjct: 845 AARNVLLAQ 853



>NP_783864
          Length = 1044

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AAHNVLLA 8
           AAHN+L+A
Sbjct: 711 AAHNLLVA 718



>XP_005262701
          Length = 1035

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AAHNVLLA 8
           AAHN+L+A
Sbjct: 702 AAHNLLVA 709



>NP_004786
          Length = 1012

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AHNVLLA 8
           AHN+LLA
Sbjct: 268 AHNLLLA 274



>NP_795362
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAHNVLLAQ 9
           A+H++LL Q
Sbjct: 305 ASHSILLIQ 313



>NP_005190
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AHNVLLAQ 9
           A NVLLAQ
Sbjct: 277 AINVLLAQ 284



>NP_001007562
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AAHNVLL 7
           AAH VLL
Sbjct: 596 AAHGVLL 602



>XP_005258573
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AAHNVLL 7
           AAH VLL
Sbjct: 596 AAHGVLL 602



>XP_005258572
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AAHNVLL 7
           AAH VLL
Sbjct: 596 AAHGVLL 602



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.123    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 472,707
Number of extensions: 487
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)