Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           17    2528
NP_001257437                                                           17    2528
NP_006281                                                              17    2528
XP_006715618                                                           17    2528
XP_005267176                                                           17    2528
NP_071765                                                              16    7355
NP_004100                                                              16    7355
NP_078966                                                              15    9605
NP_056526                                                              15    9605
XP_006723243                                                           15    9605
XP_005258890                                                           15    9605

>NP_001257436
          Length = 790

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AEASSSLS 9
           AEASSSLS
Sbjct: 545 AEASSSLS 552



>NP_001257437
          Length = 790

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AEASSSLS 9
           AEASSSLS
Sbjct: 545 AEASSSLS 552



>NP_006281
          Length = 790

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AEASSSLS 9
           AEASSSLS
Sbjct: 545 AEASSSLS 552



>XP_006715618
          Length = 577

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AEASSSLS 9
           AEASSSLS
Sbjct: 332 AEASSSLS 339



>XP_005267176
          Length = 790

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AEASSSLS 9
           AEASSSLS
Sbjct: 545 AEASSSLS 552



>NP_071765
          Length = 1386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AEASSSLS 9
           AEAS SLS
Sbjct: 335 AEASGSLS 342



>NP_004100
          Length = 184

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  AAEASSSLS 9
          +AEAS SLS
Sbjct: 46 SAEASRSLS 54



>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    AAEASSSLS 9
            A+ ASSSLS
Sbjct: 2922 ASTASSSLS 2930



>NP_056526
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    AEASSSLS 9
            A ASSSLS
Sbjct: 1262 ARASSSLS 1269



>XP_006723243
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AEASSSLS 9
           A ASSSLS
Sbjct: 902 ARASSSLS 909



>XP_005258890
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    AEASSSLS 9
            A ASSSLS
Sbjct: 1262 ARASSSLS 1269



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.265   0.0789    0.149 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 241,632
Number of extensions: 213
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)