Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              20     390
XP_005273257                                                           20     390
NP_055994                                                              17    4312
NP_001138586                                                           17    4312
NP_001265855                                                           17    4312
NP_001265856                                                           17    4312
NP_001265858                                                           17    4312
NP_064703                                                              17    4312
NP_001271266                                                           17    4312
NP_056157                                                              17    4312
XP_005248532                                                           17    4312
XP_005248531                                                           17    4312
XP_006714646                                                           17    4312
XP_006714645                                                           17    4312
XP_005247707                                                           17    4312
XP_005247706                                                           17    4312
XP_005247705                                                           17    4312
XP_006713773                                                           17    4312
NP_001036783                                                           16    5631
NP_057507                                                              16    5631
NP_001095924                                                           16    5631
NP_001095142                                                           16    5631
NP_919254                                                              16    5631
NP_061878                                                              16    5631
NP_114124                                                              16    5631
NP_001190991                                                           16    5631
NP_036203                                                              16    5631
XP_005268263                                                           16    5631
XP_005268262                                                           16    5631
XP_005268261                                                           16    5631
XP_006720227                                                           16    5631
XP_006720226                                                           16    5631
XP_005267796                                                           16    5631
XP_006720225                                                           16    5631
XP_005249841                                                           16    5631
XP_005249840                                                           16    5631
XP_005249839                                                           16    5631
XP_005249838                                                           16    5631
XP_005249837                                                           16    5631
NP_057460                                                              16    7355
NP_001244928                                                           16    7355
XP_006721607                                                           16    7355
XP_005256736                                                           16    7355
NP_001177957                                                           15    9605
NP_036602                                                              15    9605
NP_001180237                                                           15    9605
NP_116261                                                              15    9605
NP_060473                                                              15    9605
NP_001244896                                                           15    9605
NP_001231                                                              15    9605
NP_001244897                                                           15    9605
NP_940929                                                              15    9605
NP_919276                                                              15    9605
NP_001273380                                                           15    9605
XP_005254585                                                           15    9605
XP_006715974                                                           15    9605
XP_005249718                                                           15    9605
XP_006711053                                                           15    9605
XP_005246079                                                           15    9605
XP_006711052                                                           15    9605
XP_005246078                                                           15    9605
XP_006711051                                                           15    9605
XP_006711050                                                           15    9605
NP_003071                                                              15    9605
>NP_064450
          Length = 1722

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A AKKHRAD
Sbjct: 464 APAKKHRAD 472
>XP_005273257
          Length = 1726

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A AKKHRAD
Sbjct: 464 APAKKHRAD 472
>NP_055994
          Length = 1297

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AAAKKHRA 8
            A AK+HRA
Sbjct: 1040 ARAKRHRA 1047
>NP_001138586
          Length = 1236

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AAAKKHRA 8
           A AK+HRA
Sbjct: 979 ARAKRHRA 986
>NP_001265855
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 380 AKKHRA 385
>NP_001265856
          Length = 506

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 270 AKKHRA 275
>NP_001265858
          Length = 490

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 254 AKKHRA 259
>NP_064703
          Length = 526

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAKKHR 7
           AAKKHR
Sbjct: 484 AAKKHR 489
>NP_001271266
          Length = 1197

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AAAKKHRA 8
           A AK+HRA
Sbjct: 940 ARAKRHRA 947
>NP_056157
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 410 AKKHRA 415
>XP_005248532
          Length = 323

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AKKHRA 8
          AKKHRA
Sbjct: 87 AKKHRA 92
>XP_005248531
          Length = 346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 110 AKKHRA 115
>XP_006714646
          Length = 550

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 314 AKKHRA 319
>XP_006714645
          Length = 604

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AKKHRA 8
           AKKHRA
Sbjct: 368 AKKHRA 373
>XP_005247707
          Length = 299

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAKKHR 7
           AAKKHR
Sbjct: 257 AAKKHR 262
>XP_005247706
          Length = 320

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAKKHR 7
           AAKKHR
Sbjct: 278 AAKKHR 283
>XP_005247705
          Length = 371

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAKKHR 7
           AAKKHR
Sbjct: 329 AAKKHR 334
>XP_006713773
          Length = 493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AAKKHR 7
           AAKKHR
Sbjct: 451 AAKKHR 456
>NP_001036783
          Length = 1045

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AAAKKHRAD 9
           AAA  H+AD
Sbjct: 742 AAADPHKAD 750
>NP_057507
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 416 ARARRHRAE 424
>NP_001095924
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 416 ARARRHRAE 424
>NP_001095142
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 416 ARARRHRAE 424
>NP_919254
          Length = 1045

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AAAKKHRAD 9
           AAA  H+AD
Sbjct: 742 AAADPHKAD 750
>NP_061878
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>NP_114124
          Length = 252

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AAAKKHR 7
           AAAK HR
Sbjct: 122 AAAKSHR 128
>NP_001190991
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  AKKHRAD 9
          A KHRAD
Sbjct: 73 AGKHRAD 79
>NP_036203
          Length = 195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  AKKHRAD 9
          A KHRAD
Sbjct: 73 AGKHRAD 79
>XP_005268263
          Length = 1099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AAAKKHRAD 9
           AAA  H+AD
Sbjct: 742 AAADPHKAD 750
>XP_005268262
          Length = 1099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AAAKKHRAD 9
           AAA  H+AD
Sbjct: 742 AAADPHKAD 750
>XP_005268261
          Length = 1099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AAAKKHRAD 9
           AAA  H+AD
Sbjct: 742 AAADPHKAD 750
>XP_006720227
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 416 ARARRHRAE 424
>XP_006720226
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 416 ARARRHRAE 424
>XP_005267796
          Length = 768

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 462 ARARRHRAE 470
>XP_006720225
          Length = 811

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           A A++HRA+
Sbjct: 505 ARARRHRAE 513
>XP_005249841
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>XP_005249840
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>XP_005249839
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>XP_005249838
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>XP_005249837
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AAAKKHRAD 9
           A   KHRAD
Sbjct: 131 AGLDKHRAD 139
>NP_057460
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AAKKHRAD 9
           ++KKH AD
Sbjct: 334 SSKKHSAD 341
>NP_001244928
          Length = 1211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AAKKHRAD 9
           ++KKH AD
Sbjct: 376 SSKKHSAD 383
>XP_006721607
          Length = 1130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AAKKHRAD 9
           ++KKH AD
Sbjct: 334 SSKKHSAD 341
>XP_005256736
          Length = 1169

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AAKKHRAD 9
           ++KKH AD
Sbjct: 334 SSKKHSAD 341
>NP_001177957
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   KHRAD 9
           KHRAD
Sbjct: 587 KHRAD 591
>NP_036602
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   KHRAD 9
           KHRAD
Sbjct: 651 KHRAD 655
>NP_001180237
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 294 AAARRRRSD 302
>NP_116261
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 282 AAARRRRSD 290
>NP_060473
          Length = 1416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  AAAKKHRAD 9
          AAA  H AD
Sbjct: 22 AAASAHAAD 30
>NP_001244896
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AAAKKHR 7
           +AAK+HR
Sbjct: 254 SAAKEHR 260
>NP_001231
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAKKHRAD 9
           AAKK +AD
Sbjct: 263 AAKKTKAD 270
>NP_001244897
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AAAKKHR 7
           +AAK+HR
Sbjct: 270 SAAKEHR 276
>NP_940929
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  AAKKHR 7
          AA+KHR
Sbjct: 15 AARKHR 20
>NP_919276
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AAAKKHR 7
           +AAK+HR
Sbjct: 244 SAAKEHR 250
>NP_001273380
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  AAKKHR 7
          AA+KHR
Sbjct: 15 AARKHR 20
>XP_005254585
          Length = 1405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  AAAKKHRAD 9
          AAA  H AD
Sbjct: 22 AAASAHAAD 30
>XP_006715974
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   KHRAD 9
           KHRAD
Sbjct: 603 KHRAD 607
>XP_005249718
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AAAKKHR 7
           +AAK+HR
Sbjct: 152 SAAKEHR 158
>XP_006711053
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 314 AAARRRRSD 322
>XP_005246079
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 326 AAARRRRSD 334
>XP_006711052
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 333 AAARRRRSD 341
>XP_005246078
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 345 AAARRRRSD 353
>XP_006711051
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 365 AAARRRRSD 373
>XP_006711050
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AAAKKHRAD 9
           AAA++ R+D
Sbjct: 377 AAARRRRSD 385
>NP_003071
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  KHRAD 9
          KHRAD
Sbjct: 24 KHRAD 28
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.112    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 581,903
Number of extensions: 609
Number of successful extensions: 64
Number of sequences better than 10000.0: 64
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 64
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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