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Details of SAPdb entry with Sequence lFF
Primary information
SAPdb ID 1066,
PMID23422591
Peptide NameD-leu-Phe-Phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueCryo - Transmission Electron Microscopy (TEM), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy), FTIR (Fourier Transform Infrared)
SolventSodium phosphate(0.1M, pH-11.8 and pH5.7)
MethodPeptide (4.0 mg) was added to a solution of sodium phosphate 0.1 M at pH 11.8 (buffer A, 0.3 ml), and dissolution was achieved with sonication (5 min). Gelation was triggered by subsequent addition of an equal volume of 0.1 M sodium phosphate at pH 5.7 (buffer B, 0.3 ml), resulting in a final pH of 7.4.
Conc4mg/ml
TemperatureFinal - 7.4
Temperature37 °C
Year2013
Self assemblyYes
Type of Self assemblyHydrogel
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
2 - 3 hours
Hydrogel
NA
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)CO
Primary information
SAPdb ID 1111,
PMID22955637
Peptide NameL-leu-L-Phe-L-Phe
Peptide sequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
Temperature7.4
TemperatureRoom temperature
Incubation Time24 hours
Year2012
Self assemblyYes
Type of Self assemblyFibril and Globular Nano-structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanofiber, Nanosphere
150000000
None
N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O
Primary information
SAPdb ID 1112,
PMID22955637
Peptide NameD- Leu-L-Phe-L-Phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
Temperature7.4
TemperatureRoom temperature
Incubation Time24 hours- 72 hours
Year2012
Self assemblyYes
Type of Self assemblyHydrogel
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
Stable
Hydrogel
NA
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O
Primary information
SAPdb ID 1113,
PMID22955637
Peptide NameD- Leu-L-Phe-L-Phe
Peptide sequencelFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
TechniqueTransmission Electron Microscopy (TEM), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Conc10mg/ml
Temperature12
Incubation Time24 hours- 72 hours
Year2012
Self assemblyYes
Type of Self assemblyGlobular structure
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Other
3000
None
N[C@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O
Primary information
SAPdb ID 1754,
PMID30920525
Peptide NameDLeu-Phe-Phe
Peptide sequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/Conjuagatetripeptide
Conjugate partnerNone
TechniqueFourier-transformed infrared (FT-IR), circular dichroism, Thioflavin T fluorescence, transmission electron microscopy (TEM), and oscillatory rheometry
Solventsodium pHospHate buffer
Conc10 mM
Temperature 7.2± 0.1
Year2017
Self assemblyYes
Type of Self assemblyHydrogel
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Hydrogel
NA
None
N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O
Primary information
SAPdb ID 1796,
PMID30691142
Peptide NameD-Leu-Phe-Phe
Peptide sequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/Conjuagatetripeptide
Conjugate partnerNone
TechniqueFT-IR, TEM, CD
Solventbuffer solutions
Conc10 mM
Temperature 7.3± 0.1
TemperatureRoom temperature
Incubation Time30min
Year2019
Self assemblyYes
Type of Self assemblynanostructures
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Nanostructure
NA
None
N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O
Primary information
SAPdb ID 2011,
PMID28884061
Peptide NameDLeu-Phe-Phe
Peptide sequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-Terminal ModificationNone
Length3
Peptide/ConjuagatePeptide
Conjugate partnerNone
Techniquerheometry, fluorescence, circular dichro- ism (CD), FTIR spectroscopy, transmission electron microsco- py (TEM), and differential scanning calorimetry (DSC)
Solventsodium phosphate
Conc0.1 M
Temperature11.8
Year2017
Self assemblyYes
Type of Self assemblyHydrogels
Tertiary Structure
(Technique)		Not Predicted),
Linear
NA
NA
Hydrogel
NA
None
N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O