Details of SAPdb ID 1111 |
Primary information | ||
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SAPdb_ID | 1111 | |
PMID | 22955637 | |
Year | 2012 | |
Name | L-leu-L-Phe-L-Phe | |
Sequence | LFF | |
N-Terminal Modification | Free | |
C-Terminal Modification | Free | |
Non-terminal Modication | None | |
Length | 3 | |
Peptide/Conjugate/Mixture | Peptide | |
Conjugate Partner | None | |
Technique | Transmission Electron Microscopy (TEM), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD | |
Solvent | sodium phosphate buffer | |
Method | 4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel. | |
Concentration | 10mg/ml | |
pH | 7.4 | |
Temperature | Room temperature | |
Incubation Period | 24 hours | |
Self-Assembly Formation | Yes | |
Type of Self-Assembly | Fibril and Globular Nano-structure | |
Size of Self-Assembled structure | Thickness of fibers: 2 mm ; length: 150 mm , Globular structure with Diameter about 3um | |
Linear/Cyclic | Linear | |
Stability of Nanostructure | NA | |
Comment | NA | |
Secondary information | ||
Physico-Chemical properties |
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STRUCTURE |
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SMILES | N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O |