SAPdb: Entry Card with Detailed Information of Peptide

Welcome to Entry Card of Peptide


Details of SAPdb ID 1111
Primary information
SAPdb_ID1111
PMID22955637
Year2012
NameL-leu-L-Phe-L-Phe
SequenceLFF
N-Terminal ModificationFree
C-Terminal ModificationFree
Non-terminal ModicationNone
Length3
Peptide/Conjugate/MixturePeptide
Conjugate PartnerNone
TechniqueTransmission Electron Microscopy (TEM), Cryo - TEM (Transmission Electron Microscopy), AFM (Atomic Force Microscopy), CD (Circular Dichroism spectroscopy) and XRD
Solventsodium phosphate buffer
Method4.0 mg of peptide was dissolved in 300 ml of 0.1 M sodium phosphate buffer, pH 11.8 (buffer A), with the aid of sonication for 5 min, then diluted 1 : 1 with another 300 ml of 0.1 M sodium phosphate buffer, pH 5.6–5.7 (buffer B) to yield a final pH of 7.4. Samples were sonicated for another 5 min . Samples left for 24 hours for the formation of gel.
Concentration10mg/ml
pH7.4
Temperature
Room temperature
Incubation Period24 hours
Self-Assembly FormationYes
Type of Self-AssemblyFibril and Globular Nano-structure
Size of Self-Assembled structureThickness of fibers: 2 mm ; length: 150 mm , Globular structure with Diameter about 3um
Linear/CyclicLinear
Stability of NanostructureNA
CommentNA
Secondary information
Physico-Chemical properties
Physico-Chemical properties
STRUCTURE
Predicted Structure
SMILESN[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C=O