AHTpin: Designing of Antihypetensive peptides

AHTpin

Antihypertensive Peptide Inhibitors


*** Reference: Kumar et. al. (2015) An in silico platform for predicting, screening and designing of antihypertensive peptides. Sci. Rep. 5, 12512.

AHTpin Help


AHTpin is a user friendly webserver where user can submit their query as peptide, protein and multiple peptide sequences in batch mode. Detailed information about each method is given below:

Tools

CONTENTS
Dipeptide AHT activity
Tetrapeptide: Design Peptide
Tetrapeptide: Batch Submission
Tetrapeptide: Protein Scan
All in One: Variable length I/P
Propensity Matrices: P.Matrices
Propensity Matrices: PM Score Calculator

 

Dipeptide AHT activity

The page displayed in this menu gives a 20 by 20 matrix representation of the log IC50 values for the antihypertensive activity of the dipeptides. For some dipeptides (displayed in black), the values are those that are reported in literature by experimentation. Using these values, the ACE inhibition values for the other dipeptides (shown in blue) were computed using various features (amino acid composition, atomic composition and chemical descriptors)

                                                        

 

Tetrapeptide: Design Peptide

For a given query peptide, this tool first generates all possible mutations by substitutions at a single site in a sequence at a time. The mutants so generated are predicted to be inhibitors or non-inhibitors

                                                        

 

Tetrapeptide: Batch Submission

This page allows the user to submit single or multiple sequences in fasta format to predict whether the sequence(s) would be antihypertensive or not. Some additional physicochemical properties are also displayed like hydrophobicity, charge, molecular weight, etc.

                                                        

 

Tetrapeptide: Protein Scan

Protein Scan generates peptides using a 'sliding window' of fixed length from a query protein sequence. The fragments thus generated are subjected to prediction whether these would be antihypertensive or not.

                                                        

 

All in One: Variable length I/P

The models used for prediction of antihypertensive peptides in this tool have been trained on peptides of all the lengths found in literature. In other words, this prediction tool uses a model that is applicable for query peptides of any length.

                                                        

 

Propensity Matrices: P.Matrices

The tendancy of a specific amino acid to occur at a specific position in the peptide can be represented on a propensity scale. The Propensity Matrix displayed by this tool has the peptide positions as columns and amino acid as rows in the tabular form where each value represents the propensity of the amino acid to ccur at that position in the peptide.

                                                        

 

Propensity Matrices: PM Score Calculator

The PM Score Calculator allows the user to dynamically mutate residue(s) to try different combinations of amino acids in the sequence and observe the change in score as compared to the score of the original sequence. This allows the user to tailor changes that may increase or decrease the antihypertensive activity of the peptide.