| Primary information |
|---|
| SALID | SAL_22857 |
| Biomarker name | Porphyromonas endodontalis |
| Biomarker Type | Diagnostic |
| Sampling Method | Eight patients diagnosed with oropharyngeal cancer and seven patients diagnosed with hypopharyngeal cancer were recruited for this study. |
| Collection Method | Aliquot 500 ul of Saliva sample was transferred in a fresh 1.7-ml Eppendorf tube and incubated at 50 degreeC for 1 h. After incubation, 300ul of Lysozyme (3 mg/ml) was added into saliva and incubated for 1 h. |
| Analysis Method | 16S rRNA V3-V4 region sequencing |
| Collection Site | Unstimulated Saliva |
| Disease Category | Cancer |
| Disease/Condition | Squamous cell carcinoma |
| Disease Subtype | Oropharyngeal squamous cell carcinoma |
| Fold Change/ Concentration | 0.142914654 |
| Up/Downregulated | Downregulated |
| Exosomal | NA |
| Organism | Homo sapiens |
| PMID | 31832691 |
| Year of Publication | 2020 |
| Biomarker ID | 28124 |
| Biomarker Category | Microbe |
| Sequence | NZ_ACNN00000000.1 |
| Title of study | Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients |
| Abstract of study | The highest number (35.1% of global incident cases) of new oropharyngeal (OP) and hypopharyngeal (HP) cancer cases was reported in South-Central Asia. The highest incidence of HP cancer in India was reported in East Khasi Hills District of Meghalaya, Aizawl District of Mizoram, and Kamrup Urban District of Assam. HP and OP cancer showed the highest mortality rate, worst prognoses and the highest rate of nodal metastases and distant metastases. Thus, research is required to detect specific biomarkers for early prevention and diagnosis for these cancers. Oral microbiome signatures in saliva are considered as a potential diagnostic biomarker for OP and HP cancer. Bacterial profile alterations in OP and HP cancer have not been reported in India population, to establish the association of oral bacteria in the progression of OP and HP cancer; we studied bacterial communities in saliva of eight OP and seven HP cancer patients as compared to healthy controls using 16S rRNA V3-V4 region sequencing. The higher abundance of Haemophilus parainfluenzae, Haemophilus influenzae and Prevotella copri and lower abundance of Rothia mucilaginosa, Aggregatibacter segnis, Veillonella dispar, Prevotella nanceiensis, Rothia aeria, Capnocytophaga ochracea, Neisseria bacilliformis, Prevotella nigrescens and Selenomonas noxia in saliva of OP and HP cancer patients may be considered as a non-invasive diagnostic biomarker for OP and HP cancer patients. Streptococcus anginosus may be considered as a non-invasive diagnostic biomarker for OP cancer patients only. Therefore, evaluation of salivary microbial biomarkers may be informative to understand the pathobiology and carcinogenesis of OP and HP cancer. |