| Primary information |
|---|
| SALID | SAL_16955 |
| Biomarker name | Hydroxycotinine [HMDB0001390] |
| Biomarker Type | NA |
| Sampling Method | Adult (>18 years old); Gender-both |
| Collection Method | NA |
| Analysis Method | NA |
| Collection Site | Saliva |
| Disease Category | Healthy |
| Disease/Condition | Healthy |
| Disease Subtype | NA |
| Fold Change/ Concentration | <1.00 uM |
| Up/Downregulated | NA |
| Exosomal | NA |
| Organism | Homo sapiens |
| PMID | 10080645 |
| Year of Publication | 1999 |
| Biomarker ID | 107963 |
| Biomarker Category | Metabolite |
| Sequence | CN1[C@@H](C[C@H](C1=O)O)C2=CN=CC=C2 |
| Title of study | Validation of an assay for the determination of cotinine and 3-hydroxycotinine in human saliva using automated solid-phase extraction and liquid chromatography with tandem mass spectrometric detection |
| Abstract of study | The validation of a high-performance liquid chromatographic method for the simultaneous determination of low level cotinine and 3-hydroxycotinine in human saliva is reported. Analytes and deuterated internal standards were extracted from saliva samples using automated solid-phase extraction, the columns containing a hyper cross-linked styrene-divinylbenzene copolymer sorbent, and analysed by reversed-phase liquid chromatography with tandem mass spectrometric detection (LC-MS-MS). Lower limits of quantitation of 0.05 and 0.10 ng/ml for cotinine and 3-hydroxycotinine, respectively, were achieved. Intra- and inter-batch precision and accuracy values fell within +/-17% for all quality control samples, with the exception of quality control samples prepared at 0.30 ng/ml for 3-hydroxycotinine (inter-day precision 21.1%). Results from the analysis of saliva samples using this assay were consistent with subjects' self-reported environmental tobacco smoke (ETS) exposures, enhancing the applicability of cotinine as a biomarker for the assessment of low level ETS exposure. |