| Primary information |
|---|
| SALID | SAL_16409 |
| Biomarker name | Trimethylamine N-oxide |
| Biomarker Type | NA |
| Sampling Method | Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). |
| Collection Method | On average, 400 microl of unstimulated whole saliva was collected in 50 cc Falcon tubes over 5-10 min. |
| Analysis Method | CE-TOFMS |
| Collection Site | Whole Saliva |
| Disease Category | Cancer |
| Disease/Condition | Oral Cancer |
| Disease Subtype | NA |
| Fold Change/ Concentration | NA |
| Up/Downregulated | NA |
| Exosomal | NA |
| Organism | Homo sapiens |
| PMID | 28101653 |
| Year of Publication | 2017 |
| Biomarker ID | 1145 |
| Biomarker Category | Metabolite |
| Sequence | C[N+](C)(C)[O-] |
| Title of study | Effect of timing of collection of salivary metabolomic biomarkers on oral cancer detection |
| Abstract of study | The aim of this study is to evaluate the effect of duration after meals for saliva collections for oral cancer detection using metabolomics. Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). Saliva from cancer patients was collected 12 h after dinner, and 1.5 and 3.5 h after breakfast. Control subjects fasted >1.5 h prior to saliva collection. Hydrophilic metabolites were analyzed using capillary electrophoresis mass spectrometry. Levels of 51 metabolites differed significantly in controls vs. oral cancer patients at the 12-h fasting time point (P < 0.05). Fifteen and ten metabolites differed significantly at the 1.5- and 3.5-h time points, respectively. The area of under receiver operating characteristic curve for discriminating oral cancer patients from controls was greatest at the 12-h fasting time point. The collection time after meals affects levels of salivary metabolites for oral cancer screening. The 12-h fasting after dinner time point is optimal. This study contributes to design of saliva collection protocols for metabolomics-based biomarker discovery. |