| Primary information |
|---|
| SALID | SAL_16112 |
| Biomarker name | L-carnitine |
| Biomarker Type | Diagnostic |
| Sampling Method | Saliva samples were collected from OSCC patients (n = 30) during 2012 and 2013 (twenty-five males and five females, clinical stage: 4 at stage I, nine at stage II, three at stage III, and 4 teens at stage IV), whose mean age was 55y (range: 29-72y). |
| Collection Method | Approximately 2 ml of clear unstimulated whole saliva was obtained. |
| Analysis Method | HILIC-UPLC-MS |
| Collection Site | Supernatant Saliva |
| Disease Category | Cancer |
| Disease/Condition | Oral Cancer |
| Disease Subtype | Oral squamous cell carcinoma (OSCC) |
| Fold Change/ Concentration | NA |
| Up/Downregulated | Decrease |
| Exosomal | NA |
| Organism | Homo sapiens |
| PMID | 24144867 |
| Year of Publication | 2010 |
| Biomarker ID | 10917 |
| Biomarker Category | Metabolite |
| Sequence | C[N+](C)(C)C[C@@H](CC(=O)[O-])O |
| Title of study | Investigation and identification of potential biomarkers in human saliva for the early diagnosis of oral squamous cell carcinoma |
| Abstract of study | BACKGROUND: Oral cancer is 1 of the 6 most common human cancers, with an annual incidence of >300,000 cases worldwide. This study aimed to investigate potential biomarkers in human saliva to facilitate the early diagnosis of oral squamous cell carcinoma (OSCC).METHODS: Unstimulated whole saliva obtained from OSCC patients (n=30) and apparently healthy individuals (n=30) were assayed with ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) in hydrophilic interaction chromatography mode. The data were analyzed using a nonparametric Mann-Whitney U test, logistic regression, and the receiver operating characteristic (ROC) to evaluate the predictive power of each of 4 biomarkers, or combinations of biomarkers, for OSCC screening.RESULTS: Four potential salivary biomarkers demonstrated significant differences (P<0.05) in concentrations between patients at stages I-II and the healthy individuals. The area under the curve (AUC) values in control vs OSCC I-II mode based on choline, betaine, pipecolinic acid, and l-carnitine were 0.926, 0.759, 0.994, and 0.708, respectively. Four salivary biomarkers in combination yielded satisfactory accuracy (0.997), sensitivity (100%), and specificity (96.7%) in distinguishing OSCC I-II from control.CONCLUSIONS: Salivary metabolite biomarkers for the early diagnosis of OSCC were verified in this study. The proposed approach is expected to be applied as a potential technique of preclinical screening of OSCC. |