| Primary information |
|---|
| SALID | SAL_10134 |
| Biomarker name | Annexin-1 |
| Biomarker Type | Diagnostic |
| Sampling Method | 69 non-pregnant and 78 pregnant women |
| Collection Method | The supernatant saliva samples were then prepared taking 5 microliter of each sample and adding it to 195 microliter of Qubit Working solution. |
| Analysis Method | ELISA |
| Collection Site | Whole Saliva |
| Disease Category | Dental Disorder |
| Disease/Condition | Gingivitis |
| Disease Subtype | NA |
| Fold Change/ Concentration | 2 |
| Up/Downregulated | Upregulated |
| Exosomal | NA |
| Organism | Homo sapiens |
| PMID | 29608211 |
| Year of Publication | 2018 |
| Biomarker ID | P04083 |
| Biomarker Category | Protein |
| Sequence | MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN |
| Title of study | Annexin-1 as a salivary biomarker for gingivitis during pregnancy |
| Abstract of study | BACKGROUND: It is well established that there is higher susceptibility to gingival inflammation during pregnancy. Annexin-1 (ANXA1) is an anti-inflammatory protein which has been identified in gingival tissue exudates by discovery proteomics. This cross-sectional case-control study investigated the levels and association of ANXA1 and pro-inflammatory mediator interleukin (IL)-1β in the saliva of pregnant and non-pregnant women.METHODS: Whole unstimulated saliva from 69 non-pregnant and 78 pregnant women was collected prior to measurement of probing depth, clinical attachment level, bleeding on probing, and plaque. Then, the women were split into 3 subgroups depending on their periodontal status (healthy, gingivitis, and periodontitis). The levels of ANXA1 and IL-1β were measured with enzyme-linked immunosorbent assay and reported as pg/mg after normalizing against the total protein levels.RESULTS: Significantly higher ANXA1 levels were exhibited in pregnant women with gingivitis compared with health (P < 0.05) and in pregnant women with gingivitis compared with the respective non-pregnant group (P < 0.0001). There was a significantly higher level of IL-1β in gingivitis than in health in pregnant women (P < 0.05) and significantly higher levels in periodontitis compared with health in non-pregnant women (P < 0.05). Looking at the IL-1 β:ANXA1 ratio, the non-pregnant periodontitis group displayed a significantly higher ratio compared with the respective pregnant group (P < 0.05). In the non-pregnant subpopulation, the ratio was significantly higher in periodontitis compared with health (P < 0.01).CONCLUSION: Salivary ANXA1 levels are elevated in the presence of gingivitis only in pregnant, but not non-pregnant women, rendering this molecule as a potential salivary biomarker for non-invasive early screening for gingival inflammation during pregnancy. |