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PlifePred is prediction server for predicting the half-life of peptides in blood. Users can use PlifePred to increase or decrease the half-life of their peptide by generating mutants The server facilitates designing the half-life of a peptide by generating its mutants and predicting their half-life and physiochemical properties.The user can screen and select the peptide with suitable properties desired.

NATURAL

MODIFIED

Sequence Based	Analog Generation Batch Submission Protein Scan
Structure Based	Draw File

Draw
File

Designing of Half-life of Peptide using Analog Generation Module of PlifePred

In Analog Generation module, in addition to prediction of original peptide, the server also generates all possible single substitution mutants (depicted in bold and underlined) of the original peptide with their half-life and various physiochemical properties. The User can select the analogue with the desired half-life and physiochemical properties.User can further generate all possible mutants of their desired analogues obtained in above step by clicking on the peptide. This cycle can be run until the peptide with desired half-life is obtained.

Example demonstrating use of Analog Generation for Designing Half-life of Peptides

Designing of Half-life of Peptides from a Protein Sequence

Since most of the existing peptides are derived from natural proteins. PlifePred provides facility to identify potential half-life of potential peptides from a protein sequence. A tool protein scanning has been implemented to web server for the detection of putative peptide half-lives in a protein sequence. In this tool, after submission of a query protein sequence, server first generates overlapping peptides of window length selected by the user, where all the peptides will be clickable. All peptides are then predicted by the server and presented in tabular format with their half-life and physiochemical properties. User can select the peptide with desired half-life and properties. In addition, PlifePred also generates all possible single substitution mutants of the selected peptide with their half-life and physiochemical properties in a similar manner as described above. The overall approach is demonstrated below-

Example demonstrating use of Protein Scan for Designing Half-life of Peptides