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Browsing by Modifications

This page allows browsing of the different assays used to evaluate the half-life of peptides present in PEPlife 2.0 database. For more information see User Guide.

Assay Types

Modification Name Total Entries
RP-HPLC
410
Not mentioned
121
ELISA
313
Radioactivity measured by scintillation counter
4
LC/MS equipped with a microbore C-18 HPLC
9
Spectrofluorometry
5
Pulse chase experiment
8
HPLC
349
Radioimmunoassay
266
ESI-MS
8
Radioactivity measured with a gamma counter
7
Radioactivity measured by a gamma counter
1
Spectrophotometry
12
Sandwich ELISA
116
LC/MS
9
Reporter gene assay
3
Proteolysis assay
375
HPLC/liquid scintillation counting
5
Radioimmunoassay and HPLC
30
Single-dose injection technique
2
Serum decay experiment
5
Kinetic assay
1
Radioactivity measured with liquid-scintillation counter
4
LC-MS/MS
327
Radiolabelling and HPLC
1
HPLC-ESI-MS
22
ESI-LC ˆ’MS/MS
4
Radioimmunoassay and RP-HPLC
13
Sandwich immunoluminometric Assay
1
Fluorometery
2
RP-HPLC and MS-TOF
1
RP-HPLC and ESI-MS
88
HPLC and MALDI-TOF
10
Luciferase reporter assay
2
Scintigraphy
4
EIA
9
HPLC, MS-MS
15
RP-HPLC and MS-MALDI-TOF
25
MALDI-MS
1
I-125 radiolabelling method
4
Competitive Radioimmunoassay
70
BIAcore binding assay
2
MALDI-TOF-MS
52
TLC
25
Peptide specific immunoassay
1
SS Radioimmunoassay assay
2
Radioactivity decay assay (3H) .Leader peptide is radiolabelled and then decay in radioactivity is measured.
1
Radioimmunoassay and HPLC analysis
14
Gel filtration chromatography of blood plasma at different time interval show that rapid clearence of peptide from blood plasma.
12
Liquid scintillation spectrometer
3
HPLC-MS
49
HPLC , reverse phase HPLC, Partition coefficient
12
TCA precipitation
17
Antibody assay
4
Receptor-precipitable radioactivity
4
RP-HPLC and Electrochemical Detection
10
Chemiluminescence immunometric assay
3
Liquid scintillation counting
2
Autoradiography
1
Radioimmunoassay using new radioionated analogue RPPG(125I)F
1
LC/MS/MS
3
Double-antibody Radioimmunoassay
1
LC-MS
353
Radioimmunoassay and bi-exponential equation that describes a two-compartment model
8
HPLC-UV
6
Spectrofluorometer
1
Competitive ELISA
24
Radioisotopic tracing method
9
Hydrolysis and RP-HPLC
2
LC-ESI-MS
2
Bradford assay
1
HPLC and LC/ITMS
8
Radioactivity measurement
4
HPLC-ESI/MS/MS
3
MS
18
MS, ELISA
7
ELISA, LC/MS
8
HPLC radiodetection (radio-HPLC)
1
Proteolysis assay, (confirmed by Western blotting)
2
HPLC, LC-MS
1
IRM(Immunoradiometric assays)
6
Radioimmunoassay , HPLC
1
TLC (Thin-layer chromatography)
2
Radioimmunoassay, RP-HPLC
2
Radioimmunoassay, Binding protein radioactivity assay and IGF-region radioactivity
4
Radioactive assay
1
Radioimmunoassay, HPLC
4
Fluorescence Imaging
2
Radioimmunoassay ,ELISA
1
Accumulation of radioactive 3H-leu-enkephalin degradation products ,Thin layer chromatography
6
liquid chromatography-tandem mass spectrometry method
4
Radio-HPLC
11
Imaging system to measure fluorescent intensity
2
HPLC with tandem LC/MS/MS
1
Trypsin degradation assay, Analytical reverse phase HPLC
28
HPLC with fluorescence detection
2
ELISA,RP-HPLC
1
Column chromatography
1
Immunoradiometric assay (IRMA)
1
HPLC, LC-UV/MS
10
Serum stability assay
3
Double-antibody sandwich assay
3
UHPLC
12
high-performance liquidchromatography–tandem mass spectrometry method
2
N.A.
144
LC-MS/MS 
45
HPLC-MS/MS
12
Immunocapture-LC-MS/MS
3
Gamma counter
13
Mass spectrometry
18
Radioactivity assay using γ-counter
2
UPLC
8
Luminescence oxygen channelling immunoassay (LOCI)
3
ICP-MS
28
Direct ELISA
2
Fluorescence spectrophotometry
5
Triple Quadrupole Mass spectrometry
10
Dynamic T1-weighted MRI
1
RIA
31
UPLC-UV-RA
1
cELISA
6
Fluorescence assay
100
Plasma stability assay
1
GLP-2 ELISA
2
LC-MS with reverse phase liquid chromatography
1
ELISA or similar antibody based assay or LC-MS
8
Sandwich PK-ELISA
1
Modified ELISA
6
LC−MS/MS
9
Liquid scintillation counter
2
UPLC-MS/MS
10
Flow cytometry-based functional assay and mass spectrometry
2
Antibody-competition assay
1
Functional stability assay
6
instant thin layer chromatography (iTLC)
2
LC-HRMS
22
ELISA (measure mAM)
1
ELISA (measure mAM )
2
ELISA (measure tAM )
3
Densitometry analysis using NIH Image J
2
IVIS at 750/780 nm
1
UPLC-MS
49
ICP‐MS
1
ELISA, LC-MS/MS
4
Insulin receptor ELISA
6
HPLC–MS/MS
15
Laser capture/mass spectrometry
2
HPLC analysis with fluorescence detection
4
MS using MALDI-TOF
1
GFP-specific ELISA
7
Zymography
5
HRAM LC/MS
6
radio-HPLC 
4
CHX assay, Western blotting
2
LC–MS/MS 
2
1H-NMR
2
UPLC-MS 
3
Radioactivity assay
16
SEC
2
Fluorescence spectrometry
16
Dosimetry
4
Western blotting
10
UPLC–MS/MS
13
LCHRMS
11
Two site ELISA 
3
Radiochromatography
1
Chromogenic activity assays
14
sandwich ELISA 
2
ELISA 
2
OCN ELISA
2
Scintillation proximity assay
13
flow cytometry
1
Glo-sensor transfected cell-based luminescence assay
7
HIV inhibition assay
2
BCA protein assay
9
In vitro cell based activity assay
6
RP-UPLC
6
PET scanning
1
2-site immunoassay
4
Waters Xevo TQ-MS
14
Uric acid degradation assay
4
Fluorescent assay
1
LC-MS, ELISA
2
Indirect competitive ELISA
1
Confocal microscopy
6
In vivo imaging system
2
LC–MS/MS
37
Western blotting and spot densitometry
1
Flurorescence assay
1
Tandem mass spectrometry (MS)
2
Surface Plasmon Resonance (SPR) 
7
Standard assay
8
Fluorescence in vivo imaging
8
Methylene blue method 
5
Hydrogen Exchange Mass Spectrometry (HX-MS)
20
HPLC, MS
3
HPLC-MS/MS 
1
LC-MS with electrospray ionization assay
4
LC-MS 
7
LC-MS/MS.
21
LC‐MS/MS
4
LC-MS/ MS
3
Radioiodination assay
4
UV-HPLC and LC-ESI-MS
4
LC-MS/MS using an Agilent HPLC
8
UHPLC coupled with Quadrupole Time-of-Flight (Q-TOF) Mass Spectrometry
4
Ultra-High Performance Liquid Chromatography (UHPLC)
2
IC-ELISA
1
BD cytoflex flow cytometry
1
RP-HPLC-FD
4
PET-CT imaging
1
AlphaLISA
4
Luminescent Oxygen Channeling Immunoassay 
2
RP-HPLC using a fluorescence detector
7
RP-HPLC using fluorescence detector
2
A single quadrupole liquid chromatography mass spectrometer 
1
LC-MS/SIM
1
electrospray ionization LC–MS/MS
1
Exenatide ELISA
2
Anti-PEG ELISA
1
RP-HPLC-ESI-MS
4
IDS-iSys chemiluminescence assay
4
Human TGF-b RII Duo Set ELISA 
2
GLP-2 (1–33) specific radioimmunoassay
2
LC-HRMS.
3
MALDI-TOF-MSI and LC-MS/MS
1
whole-body fluorescence imaging 
8
LC‐MS/MS assay
2
Cycloheximide chase assay and Western blot
2
HPLC using a fluorescence detector
1
IR scanning using anti-biotin antibody 
1
Immunoenzymetric assay
2
Exendin-4 EIA 
2
RIA (total PYY assay)
1
RIA (when correcting for NH2-terminal degradation)
1
RIA (when we corrected for NH2-terminal degradation)
1
SDS PAGE
1
Ultraviolet spectroscopy
3
MALDI-TOF MS
2
Enzyme activity assay
6
Resorufin-derived absorbance assay
1
Bioassay
2
MTT
2
NA
3
aPTT-based assay
1
cAMP assay
4
enzyme immunoassay
4
cell-based bioassay
1