Welcome to Sequence Card of Peptide
Details of CPPsite entry with Sequence GRKKRRQRRR |
| Primary information | |
|---|---|
| PEPTIDE SEQUENCE | GRKKRRQRRR |
| CPPsite ID | 2475, 2558, 2559, 2677, 2678, 2822, 1005, |
| PEPTIDE NAME | Cys(BSH)-TAT, Tm3+-DOTA-CAT, Gd3+-DOTA-CAT, Tat, γ-AApeptide (mimetic of Tat), Tat (TG), Tat (48-57) |
| CHIRALITY | L, L, L, L, Modified, L, L |
| LINEAR/CYCLIC | Linear, Linear, Linear, Linear, Linear, Linear, Linear |
| SOURCE | Protein derived, Protein derived, Protein derived, Protein derived, Synthetic, Protein derived, Protein derived |
| CATEGORY | Cationic, Cationic, Cationic, Cationic, Cationic, Cationic, Cationic |
| SUB CELLULAR LOCALIZATION | Cytoplasm, NA, NA, Cytoplasm and Nucleus, Cytoplasm and Nucleus, NA, Cytoplasm and nucleus, nucelolus |
| N-TERMINAL MODIFICATION | Addition of cysteine and BSH, Conjugated with Tm³?-DOTA via CAT recognition site, Conjugated with Gd³?-DOTA via CAT recognition site, Conjugation with fluorescein, Conjugation with fluorescein, NA, Labelled with Cys-acetamidomethyl |
| C-TERMINAL MODIFICATION | NA, Free, Free, NA, NA, NA, Conjugation with fluorescein maleimide |
| CHEMICAL MODIFICATION | NA, NA, NA, NA, NA, NA, NA |
| UPTAKE EFFICIENCY | Lower uptake efficiency than 8BSH-11R, Cat-D expression increases uptake of Tm3+-DOTA-CAT in neuronal SN56 cells, NA, NA, NA, NA, High |
| PROPOSED UPTAKE MECHANISM | NA, NA, NA, Endocytosis, Endocytosis, NA, Non-endocytic pathway |
| INVITRO/INVIVO | In vitro and in vivo, In vitro and in vivo, In vitro and in vivo, In vitro, In vitro, In vitro and in vivo, In vitro |
| CELL LINE | U87 DEGFR and PA U87 glioma, SN56 cells, SN56 cells, Jurkat and HeLa cells, Jurkat and HeLa cells, H9c2 cells, HeLa |
| In VIVO MODEL | Female nude mice (15e20 g, BALB/c Slc-nu/nu), APP/PS1-dE9 mice, APP/PS1-dE9 mice, NA, NA, Streptozotocin-induced diabetic mouse model, NA |
| CARGO | BSH, MRI probe [DOTA-caged metal ion (Tm³?)], MRI probe [DOTA-caged metal ion (Gd³?)], Fluorophore (Fluorescein), Fluorophore (Fluorescein), Protein (GFP, anti-oxidative metallothionein protein), Fluorophore (fluorescein) |
| PMID | 24452095, 23281285, 23281285, 22413929, 22413929, 23871961 |
| PATENT | Unknown, Unknown, Unknown, Unknown, Unknown, Unknown, US 20100216716 |
| Tertiary Structure (Technique) | 2475 (PEPstrMOD), 2558 (PEPstrMOD), 2559 (PEPstrMOD), 2677 (PEPstrMOD), 2678 (PEPstrMOD), 2822 (PEPstrMOD), 1005 (PEPstrMOD), |