AptBacterialDB

Cross-Referenced Aptamer Databases

This table lists AptBacterialDB entries that are cross-referenced in external databases such as AptaDB, Aptabase and UTexas Aptamer Database. It provides a direct mapping between AptBacterialDB IDs and their corresponding records in these resources. This page displays two tables: the first for entries sourced from AptaDB and Aptabase, and the second for UTexas. Click on the database names or column headers to navigate to their respective external sources for detailed information.

AptaDB and Aptabase Database Entries

AptBacDB ID AptaDB ID Aptabase ID PMID/DOI Aptamer Name Sequence (5′ to 3′)
ABdb_0009 52 AbID_1 15023071 F1-1 GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-ACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGU-CAUAUGUGCGUCUACAUGGAUCCUCA
ABdb_0010 53 N/A 15023071 F2-1 GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-UCCCUGGCCCAAGAUCCUAAUAAAGUUUUUUCGGACCGGAGCGAAACCACUAUCCUCUUAAGCAAUCUGU-CAUAUGUGCGUCUACAUGGAUCCUCA
ABdb_0011 54 N/A 15023071 F3-1 GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUACAACACAUCAUUACGGCUGCUAUUGGCUCCAAGCGUCUUUCUCCCUGGUCAAUAGUCCAGCCACCACG-CAUAUGUGCGUCUACAUGGAUCCUCA
ABdb_0012 55 N/A 15023071 F4-1 GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGAGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA
ABdb_0015 941 AbID_2 16189080 S-PS8.4 GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UCACUGUUAUCCGAUAGCAGCGCGGGAUGA-GGGUCAAUGCGUCAUA
ABdb_0024 285 AbID_3 17188871 Class II-1 GGACCGAGAAGUUACCCUGUAAUCUUAGGAUGAAUCGCAUGCUCUAGCGACCUUUUCGGCUUCGGCGUACGCACAUCGCAGCAAC
ABdb_0025 901 AbID_4 17265180 Aptamer CATCCGTCACACCTGCTCTGGCCACTAACATGGGGACCAGGTGGTGTTGGCTCCCGTATC
ABdb_0042 908 AbID_5 18803393 hemag1P AGCAGCACAGAGGTCAGATG-TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTG-CCTATGCGTGCTACCGTGAA
ABdb_0049 905 AbID_6 19498077 SA20 GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA
ABdb_0050 904 AbID_7 19498077 SA23 GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA
ABdb_0051 906 N/A 19498077 SA31 GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA
ABdb_0052 907 N/A 19498077 SA34 GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA
ABdb_0065 428 AbID_8 19284297 M64CA (aptamers 20, 34, 41, 46, and 50 ) GGGAGCTCAGAATAAACGCTCAA-TTCGGGAATGATTATCAAATTTATGCCCTCTGAT-TTCGACATGAGGCCCGGATC
ABdb_0066 429 AbID_9 19284297 Aptamer 11 (named M64RA) GGGAGCTCAGAATAAACGCTCAA-TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA-TTCGACATGAGGCCCGGATC
ABdb_0075 1179 N/A 20452328 S132B-C11 GGGAGGAGGAGAGAUGUGAACUU-AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG-AGAAACUCUACACUGGACUGGCG
ABdb_0076 1178 N/A 20452328 S132B-C12 GGGAGGAGGAGAGAUGUGAACUU-ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA-AGAAACUCUACACUGGACUGGCG
ABdb_0077 1177 AbID_10 20452328 S132B-C22 GGGAGGAGGAGAGAUGUGAACUU-GACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGC-AGAAACUCUACACUGGACUGGCG
ABdb_0078 291 AbID_11 20337767 A8 ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT
ABdb_0086 978 N/A 21627466 Aptamer 19 CGTACGGTCGACGCTAGC-GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC-CACGTGGAGCTCGGATCC
ABdb_0087 979 N/A 21627466 Aptamer 31 CGTACGGTCGACGCTAGC-ACACTCTTTTGCTCGTGTTTTTGCCTGTTACATAAAATGAATCAGTGGATGTTTCCTTCT-CACGTGGAGCTCGGATCC
ABdb_0088 980 N/A 21627466 Aptamer 7 CGTACGGTCGACGCTAGC-GGCGGCCGTGAAATTTGCCAAATGCCGTCTTGGCTTTCGCCCAATGTATCCTGGGTGTTC-CACGTGGAGCTCGGATCC
ABdb_0089 981 AbID_12 21627466 Aptamer 37 CGTACGGTCGACGCTAGC-GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC-CACGTGGAGCTCGGATCC
ABdb_0090 893 N/A 22166202 Two Arm ( from clone I-1) GGGUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAA
ABdb_0091 909 N/A 22166202 Clone I-1 GGGAUACCAGCUUAUUCAAUU-UGAUUCCAUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAU-AGAUAGUAAGUGCAAUCU
ABdb_0103 N/A AbID_13 21743920 1–PA GCGCGGATCCCGCGC-GATGTGGGTGTAGTTGGAGGGTAAACGTT-CGCGCGAAGCTTGCG
ABdb_0104 N/A AbID_14 21743920 2–PA GCGCGGATCCCGCGC-CAGACCGTAAGGGATGCCGCCTAAACACC-CGCGCGAAGCTTGCG
ABdb_0124 902 AbID_15 22310030 S25 GGGUUCACUGCAGACUUGACGAAGCUU-GAGAGAUGCCCCCUGAUGTGCAUUCUUGUUGUGUUGCGGC-AAUGGAUCCACAUCTACGAAUUC
ABdb_0125 897 AbID_16 22480209 A3P ATAGGAGTCACGACGACCAGAA-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-TATGTGCGTCTACCTCTTGACTAAT
ABdb_0126 899 N/A 22480209 A1 TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG
ABdb_0127 898 N/A 22480209 A1P ATAGGAGTCACGACGACCAGAA-TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG-TATGTGCGTCTACCTCTTGACTAAT
ABdb_0128 900 N/A 22480209 A3 TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT
ABdb_0132 128 AbID_17 22438927 APT(SEB1) GGTATTGAGGGTCGCATC-CACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGT-GATGGCTCTAACTCTCCTCT
ABdb_0133 129 N/A 22438927 APT(SEB2) GGTATTGAGGGTCGCATC-CCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCG-GATGGCTCTAACTCTCCTCT
ABdb_0134 910 AbID_18 22218972 BAS-6F ATACGGGAGCCAACACCATCCCTCTTAGGATACAAAGCCAAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT
ABdb_0135 912 N/A 22218972 BAS-6R ATCCGTCACACCTGCTCT-GCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGA-TGGTGTTGGCTCCCGTAT
ABdb_0147 678 N/A 22370280 B1 CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA-GGAGACGAGATAGGCGGACACT
ABdb_0148 679 AbID_19 22370280 B2 CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA-GGAGACGAGATAGGCGGACACT
ABdb_0149 680 N/A 22370280 B3 CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGTTGGCCAGATGATATAAAGGGTCAACCCCCCA-GGAGACGAGATAGGCGGACACT
ABdb_0150 681 N/A 22370280 B4 CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCG-GGAGACGAGATAGGCGGACACT
ABdb_0151 682 N/A 22370280 B5 CTTCTGCCCGCCTCCTTCC-CTAAGCACAGGGAAACCAGCTAATGAGTTAGGCCTGTCCCCCACG-GGAGACGAGATAGGCGGACACT
ABdb_0152 683 N/A 22370280 B6 CTTCTGCCCGCCTCCTTCC-CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG-GGAGACGAGATAGGCGGACACT
ABdb_0153 684 N/A 22370280 B7 CTTCTGCCCGCCTCCTTCC-TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG-GGAGACGAGATAGGCGGACACT
ABdb_0154 685 N/A 22370280 B8 CTTCTGCCCGCCTCCTTCC-ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA-GGAGACGAGATAGGCGGACACT
ABdb_0155 686 N/A 22370280 B9 CTTCTGCCCGCCTCCTTCC-ACCGATCCATCGAGTTTCTGAGAAAGGCCCGGAGAAACCGCGAGA-GGAGACGAGATAGGCGGACACT
ABdb_0156 687 N/A 22370280 B10 CTTCTGCCCGCCTCCTTCC-TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA-GGAGACGAGATAGGCGGACACT
ABdb_0263 N/A AbID_20 https:doi.org10.1039C3AY41576G A1 AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA
ABdb_0264 N/A AbID_21 https:doi.org10.1039C3AY41576G A15/40 AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA
ABdb_0265 N/A AbID_22 https:doi.org10.1039C3AY41576G A23.2 AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA
ABdb_0273 1200 AbID_23 23676911 Clone I-2 GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC
ABdb_0275 N/A AbID_24 https://doi.org/10.1007/s13213-013-0720-z Clone #2 GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC
ABdb_0356 1206 AbID_25 25185503 Antibac1 TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC
ABdb_0357 1207 N/A 25185503 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0387 962 AbID_26 24417693 WKB-14 GCTGCAATACTCATGGACAG-GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC-GTCTGGAGTACGACCCTGAA
ABdb_0388 961 N/A 24417693 WKB-3 GCTGCAATACTCATGGACAG-GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG-GTCTGGAGTACGACCCTGAA
ABdb_0389 963 N/A 24417693 WKB-51 GCTGCAATACTCATGGACAG-GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC-GTCTGGAGTACGACCCTGAA
ABdb_0761 917 N/A 27104834 P12-52 CATACGATTTAGGTGACACTATAG-CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG-ATTTCTCCTACTGGGATAGGTGGA
ABdb_0762 915 N/A 27104834 P12-31 CATACGATTTAGGTGACACTATAG-CCCTCCGGGGGGGGGGGTCATCGGGATACCTGGTAAGGATA-ATTTCTCCTACTGGGATAGGTGGA
ABdb_0763 918 N/A 27104834 P12-55 CATACGATTTAGGTGACACTATAG-CCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA-ATTTCTCCTACTGGGATAGGTGGA
ABdb_0764 916 N/A 27104834 P12-17 CATACGATTTAGGTGACACTATAG-GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG-ATTTCTCCTACTGGGATAGGTGGA
ABdb_0851 903 AbID_27 28441340 CCFM641-5 AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA
ABdb_0918 N/A AbID_28 28119514 Antibac1 TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC
ABdb_0919 524 N/A 28119514 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0919 525 N/A 28119514 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0919 528 N/A 28119514 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0919 532 N/A 28119514 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0919 642 N/A 28119514 Antibac2 TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC
ABdb_0920 1261 N/A 28689112 Aptamer 1 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0921 1262 N/A 28689112 Aptamer 2 GGGAGCTCAGAATAAACGCTCAA-GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG-TTCGACATGAGGCCCGGATC
ABdb_0922 1263 N/A 28689112 Aptamer 3 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0923 1264 N/A 28689112 Aptamer 4 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0924 1265 N/A 28689112 Aptamer 5 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0925 1266 N/A 28689112 Aptamer 6 GGGAGCTCAGAATAAACGCTCAA-GGCACGATGTGGCTACATCGATCGCGGTACTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0926 1267 N/A 28689112 Aptamer 7 GGGAGCTCAGAATAAACGCTCAA-GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG-TTCGACATGAGGCCCGGATC
ABdb_0927 1268 N/A 28689112 Aptamer 8 GGGAGCTCAGAATAAACGCTCAA-GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG-TTCGACATGAGGCCCGGATC
ABdb_0928 1269 N/A 28689112 Aptamer 9 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0929 1270 N/A 28689112 Aptamer10 GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAGGTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0930 1271 N/A 28689112 Aptamer11 GGGAGCTCAGAATAAACGCTCAA-GGCACGATGTGGCTACATCGATCGCGGTACTGGTG-TTCGACATGAGGCCCGGATC
ABdb_0931 1272 N/A 28689112 Aptamer12 GGGAGCTCAGAATAAACGCTCAA-GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG-TTCGACATGAGGCCCGGATC
ABdb_1029 1251 N/A 29698672 S-12 AGCAGCACAGAGGTCAGATG-GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG-CCTATGCGTGCTACCGTGAA
ABdb_1032 1250 N/A 29698672 S-10 AGCAGCACAGAGGTCAGATG-CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC-CCTATGCGTGCTACCGTGAA
ABdb_1035 1252 N/A 29698672 S-26 AGCAGCACAGAGGTCAGATG-TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC-CCTATGCGTGCTACCGTGAA
ABdb_1036 1253 N/A 29698672 S-28 AGCAGCACAGAGGTCAGATG-TAATACTACCAACTTCTTTGCCTGGCGTAAGTAACAGTCA-CCTATGCGTGCTACCGTGAA
ABdb_1052 922 N/A 29756774 Apt-1 TGAGCCCAAGCCCTGGTATG-TTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGG-GGCAGGTCTACTTTGGGATC
ABdb_1053 923 N/A 29756774 Apt-2 TGAGCCCAAGCCCTGGTATG-TGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAG-GGCAGGTCTACTTTGGGATC
ABdb_1054 924 N/A 29756774 Apt-3 TGAGCCCAAGCCCTGGTATG-TAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGG-GGCAGGTCTACTTTGGGATC
ABdb_1055 925 N/A 29756774 Apt-4 TGAGCCCAAGCCCTGGTATG-AGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATT-GGCAGGTCTACTTTGGGATC
ABdb_1056 926 N/A 29756774 Apt-5 TGAGCCCAAGCCCTGGTATG-CGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTT-GGCAGGTCTACTTTGGGATC
ABdb_1057 927 N/A 29756774 Apt-6 TGAGCCCAAGCCCTGGTATG-GTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGG-GGCAGGTCTACTTTGGGATC
ABdb_1058 928 N/A 29756774 Apt-7 TGAGCCCAAGCCCTGGTATG-TGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGG-GGCAGGTCTACTTTGGGATC
ABdb_1059 929 N/A 29756774 Apt-8 TGAGCCCAAGCCCTGGTATG-TCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCA-GGCAGGTCTACTTTGGGATC
ABdb_1060 930 N/A 29756774 Apt-9 TGAGCCCAAGCCCTGGTATG-TTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAG-GGCAGGTCTACTTTGGGATC
ABdb_1061 1158 N/A 30477331 SE40 TACGACTCACTATAGGGATCC-ACCTATGGCAGATTGAGCCCAAGGGCTGTGCAGC-GAATTCCCTTTAGTGAGGGTT
ABdb_1062 1159 N/A 30477331 SE42 TACGACTCACTATAGGGATCC-CCCCGAGTGAAGAGCAGGACAGCGGGACAGCGTC-GAATTCCCTTTAGTGAGGGTT
ABdb_1063 1160 N/A 30477331 SE43 TACGACTCACTATAGGGATCC-GTGACTGTACGGGCTCAGTCGTTACTTGAGAGTT-GAATTCCCTTTAGTGAGGGTT
ABdb_1064 1161 N/A 30477331 SE48 TACGACTCACTATAGGGATCC-AATGGCACAGCGCCTGGAACGTACTCTGTACCTG-GAATTCCCTTTAGTGAGGGTT
ABdb_1065 1162 N/A 30477331 SE52 TACGACTCACTATAGGGATCC-TTCGCATCCGGCACGATGGCTAGGACACCCCGAT-GAATTCCCTTTAGTGAGGGTT
ABdb_1357 919 N/A 31837967 20–5 GCAATGGTACGGTACTTCC-ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT-CAAAAGTGCACGCTACTTTGCTAA
ABdb_1358 920 N/A 31837967 20–7 GCAATGGTACGGTACTTCC-CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG-CAAAAGTGCACGCTACTTTGCTAA
ABdb_1359 921 N/A 31837967 20–10 GCAATGGTACGGTACTTCC-TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC-CAAAAGTGCACGCTACTTTGCTAA
ABdb_1367 1079 N/A 32499557 R10C5 GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA
ABdb_1368 1080 N/A 32499557 R10C1 GCAATGGTACGGTACTTCCCCACCCCACGCTGCTCCCAAAAGTGCACGCTACTTTGCTAA
ABdb_1393 1031 N/A 33262379 EF508 TAGGGAAGAGAAGGACATATGAT-ACTGGCCTTGACACCCTGTTGTGGCTTGATGACAATAACA-TTGACTAGTACATGACCACTTGA
ABdb_1525 1069 N/A 33585756 HPA-2 AAGGAGCAGCGTGGAGGTTA-CCAGGAGGACCCTATTCTCGTGTATCGACGAGATCCAGTG-ACCACGACGACACACCCTAA
ABdb_1528 1070 N/A 33585756 HPA-4 AAGGAGCAGCGTGGAGGTTA-CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG-ACCACGACGACACACCCTAA

UTexas Aptamer Database Entries

AptBacDB_ID PubMed ID Type of Nucleic Acid Name of Aptamer Target Aptamer Sequence Sequence Length GC Content Affinity Kd (nM) Pool Type Pool Random Region Binding Buffer/Conditions Divalent Salt Type of the buffer pH Molecular weight of target Application as quoted in the referenced paper Post-selex modifications to the aptamer Additional Information Serial Number Aptagen Cross Referencing
ABdb_0001 12799428 ssDNASpiegelmer B12b10_65D‐staphylococcal enterotoxin B peptide (respective SEB peptides) 5'GACGTCTTCGAATCCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTC3' 650.5846153846Kd: 200 ± 20 nM2005′‐TCAGCTGGACGTCTTCGAAT‐N60‐TGTCAGGAGCTCGAATTCCC‐3′6020 mM Tris, pH 7.4; 250 mM NaCl; 5 mM KCl; 2 mM CaCl2; 1 mM MgCl2; 0.005% Triton X‐100MgCl/CaClTris Buffers7.428 kDa proteinDiagnostic: " In order to generate a Spiegelmer with wide application potential for the diagnosis and/or treatment of SEB mediated diseases, we envisaged a domain approach to perform the mirror-image in vitro selection. diagnosis and/or treatment of SEB mediated diseases"Truncated version of b12b10 (65 nts)10000406
ABdb_0001 12799428 ssDNASpiegelmer B12b10_65D‐staphylococcal enterotoxin B peptide ( full-length SEB protein) 5'GACGTCTTCGAATCCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTC3' 650.5846153846Kd: 420 nM4205′‐TCAGCTGGACGTCTTCGAAT‐N60‐TGTCAGGAGCTCGAATTCCC‐3′6020 mM Tris, pH 7.4; 250 mM NaCl; 5 mM KCl; 2 mM CaCl2; 1 mM MgCl2; 0.005% Triton X‐100MgCl/CaClTris Buffers7.428 kDa proteinDiagnostic: " In order to generate a Spiegelmer with wide application potential for the diagnosis and/or treatment of SEB mediated diseases, we envisaged a domain approach to perform the mirror-image in vitro selection. diagnosis and/or treatment of SEB mediated diseases"Truncated version of b12b10 (65 nts)10000406
ABdb_0009 15023071 ssRNAF1-1C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGUCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1210.5619834711Kd: 2±11 nM25‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants (45)."Not applicable1000045375
ABdb_0010 15023071 ssRNAF2-1C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUUCCCUGGCCCAAGAUCCUAAUAAAGUUUUUUCGGACCGGAGCGAAACCACUAUCCUCUUAAGCAAUCUGUCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1190.4705882353Kd: 14±2 nM145‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants (45)."Not applicable10000454
ABdb_0011 15023071 ssRNAF3-1C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUGUACAACACAUCAUUACGGCUGCUAUUGGCUCCAAGCGUCUUUCUCCCUGGUCAAUAGUCCAGCCACCACGCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1200.5083333333Kd: 4±15 nM45‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants (45)."Not applicable10000455
ABdb_0012 15023071 ssRNAF4-1C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUGACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGAGGGCUCGGACUCUGAUUGCCUCCCCGCACCCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1190.512605042Kd: 7±1 nM75‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants (45)."Not applicable10000456
ABdb_0014 15023071 ssRNAF4-2C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUGACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGGGGGCUCGGACUCUGAUUGCCUCCCCGCACCCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1190.5210084034Kd: 294±170 nM2945‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants (45)."Not applicable10000457
ABdb_0013 15023071 ssRNAF5-1C-terminal ribonuclease domain of colicin E3 (CRD of colicin E3) 5‘GGGAGAAUUCCGACCAGAAGCUUGUCAGCUGCUCGCGGGAUCGAUCCAUUCGGUGGCCAUGCUCCGGAAGACGGGCCGGCUUCGCAAGACUCAGGCAUAUGUGCGUCUACAUGGAUCCUCA3‘ 1210.5867768595Kd: 10±1 nM105‘-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3‘7210 mM Tris-HCl (pH 7.6), 10 mM MgCl2, and 30 mM NH4ClMgClTris Buffers7.6Not reportedDetection: " Aptamer F2-1 could be used for the structural analysis of the interaction with colicin E3, in place of 16S rRNA. Furthermore, the aptamers work as analogues of the colicin E3 immunity protein, and the expression of the aptamers in a colicin-sensitive cell could confer resistance to colicin E3. This implies that the aptamers could be used as selection markers, such as anti-aminoglycoside aptamers, for the isolation of certain E. coli mutants."Not applicable10000458
ABdb_0015 16189080 ssRNAS-PS8.4Type IVB pili of Salmonella enterica (S. enterica) serovar Typhi (pre-PilS) 5′GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUA3' 880.5113636364Kd: 8.56 nM8.565′-GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-N30-GGGUCAAUGCGUCAUA-330Binding Buffer For Assay: 25 mM Tris-HCl, 50 mM KCl, 200 mM NaCl, 0.2 mM EDTA, 5% [vol/vol] glycerol, 0.5 mM DTT, pH 7.5NoneTris Buffers7.5Not reportedTherapeutic: " Analysis of bacterial type IVB pilus-host cell interactions, may yield information for the development of putative new drugs against S. enterica serovar Typhi bacterial infections, useful both in prevention of infection and in therapeutic treatment."Not applicable10000569
ABdb_0024 17188871 ssRNAII-1Release factor 1, Escherichia coli (E. Coli) 5'GGACCGAGAAGYYACCCAGGAUUGCGUGUUAAGGCGCUCGGCUCGAUAUUUAUGCUGGUCAUGGUGGACGCACAUCGCAGCAAC3' 820.5609756098Kd: 30 ± 6 nM305'-GGACCGAGAAGYYACCC-N50-ACGCACATCGCAGCAAC-3'50Not reportedNoneNot ReportedN/ANot reportedTherapeutic: " A pool of 84-nt RNAs containing a randomized sequence of 50 nt was selected against gel-immobilized Escherichia coli release factor 1 (RF-1) responsible for translation termination at amber (UAG) stop codon. By binding to and hence inhibiting the action of RF-1 specifically or bio-orthogonally, aptamer class II-1 enhanced the amber suppression efficiency in the presence of an anticodon-adjusted (CUA) suppressor tRNA without practically damaging the protein translation machinery of the cell-free extract of E. coli, as confirmed by the translation of amber-mutated (gfp(amber141) or gfp(amber178)) and wild-type (gfp(wild)) genes of GFP."Not applicable10000635
ABdb_0077 20452328 2'-fluoro-RNAS132B-C22Light chain of type A botulinum neurotoxin (BoNT/A) (LCA) 5′GGGAGGAGGAGAGAUGUGAACUUGACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGCAGAAACUCUACACUGGACUGGCG3′ 930.5161290323Kd: 87 ± 20 nM875′-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3′47PBS pH 7.4, 300 mM NaCl, 3 mM MgCl2, 5 mM DTT 1 mg/ml BSA, 0.2% Tween, 1 mg/ml heparinMgClPBS/phosphate buffers7.4150 kDaTherapeutic: " The category A agent, botulinum neurotoxin (BoNT), is the most toxic molecule known to mankind. The endopeptidase activity of light chain domain of BoNT is the cause for the inhibition of the neurotransmitter release and the flaccid paralysis that leads to lethality in botulism. These data on K(I) and IC(50) strongly suggest that the aptamers have strong potential as antidotes that can reverse the symptom caused by BoNT/A."2′-fluoro-pyrimidin modified nucleic acid (2′-F-CTP and 2′-F-UTP) during in vitro transcription step to generate 2′-F-modified RNA10000795528
ABdb_0076 20452328 2'-fluoro-RNAS132B-C12Light chain of type A botulinum neurotoxin (BoNT/A) (LCA) 5′GGGAGGAGGAGAGAUGUGAACUUACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCAAGAAACUCUACACUGGACUGGCG3′ 910.5054945055Kd: 111 ± 21 nM1115′-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3′47PBS pH 7.4, 300 mM NaCl, 3 mM MgCl2, 5 mM DTT 1 mg/ml BSA, 0.2% Tween, 1 mg/ml heparinMgClPBS/phosphate buffers7.4150 kDaTherapeutic: " The category A agent, botulinum neurotoxin (BoNT), is the most toxic molecule known to mankind. The endopeptidase activity of light chain domain of BoNT is the cause for the inhibition of the neurotransmitter release and the flaccid paralysis that leads to lethality in botulism. These data on K(I) and IC(50) strongly suggest that the aptamers have strong potential as antidotes that can reverse the symptom caused by BoNT/A."2′-fluoro-pyrimidin modified nucleic acid (2′-F-CTP and 2′-F-UTP) during in vitro transcription step to generate 2′-F-modified RNA10000796
ABdb_0075 20452328 2'-fluoro-RNAS132B-C11Light chain of type A botulinum neurotoxin (BoNT/A) (LCA) 5′GGGAGGAGGAGAGAUGUGAACUUAUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACGAGAAACUCUACACUGGACUGGCG3′ 930.5053763441Kd: 186 ± 18 nM1865′-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3′47PBS pH 7.4, 300 mM NaCl, 3 mM MgCl2, 5 mM DTT 1 mg/ml BSA, 0.2% Tween, 1 mg/ml heparinMgClPBS/phosphate buffers7.4150 kDaTherapeutic: " The category A agent, botulinum neurotoxin (BoNT), is the most toxic molecule known to mankind. The endopeptidase activity of light chain domain of BoNT is the cause for the inhibition of the neurotransmitter release and the flaccid paralysis that leads to lethality in botulism. These data on K(I) and IC(50) strongly suggest that the aptamers have strong potential as antidotes that can reverse the symptom caused by BoNT/A."2′-fluoro-pyrimidin modified nucleic acid (2′-F-CTP and 2′-F-UTP) during in vitro transcription step to generate 2′-F-modified RNA10000797
ABdb_0093 21504182 ssDNA20A24PGroup A Streptococcus (GAS)* 5′AGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA3′ 800.575Kd: 9.1 ± 0.6 nM9.15′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableThis aptamer was noted as "particulalry promising." A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. One of the recommended aptamers for this article. 5' fam addition10000830
ABdb_0094 21504182 ssDNA15A3PGroup A Streptococcus (GAS)* 5'TTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGGCATCTGACCTCTGTGCTGCT3' 800.5875Kd: 9.6 ± 0.3 nM9.65′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableThis aptamer was noted as "particulalry promising." A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. One of the recommended aptamers for this article. 5' fam addition10000831
ABdb_0095 21504182 ssDNA20A1Group A Streptococcus (GAS)* 5′AGCAGCACAGAGGTCAGATGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCCTATGCGTGCTACCGTGAA3′ 800.6Not reportedN/A5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2NoneTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Primer regions removed (no primer version of 20a1p)A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000832
ABdb_0096 21504182 ssDNA20A1PGroup A Streptococcus (GAS)* 5′TTCACGGTAGCACGCATAGGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCATCTGACCTCTGTGCTGCT3′ 800.6Not reportedN/A5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2NoneTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableA total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000833
ABdb_0097 21504182 ssDNA20A8Group A Streptococcus (GAS)* 5'CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC3' 400.55Kd: 4 ± 1 nM45′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Primer regions removed (no primer version of 20a8p)A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000834
ABdb_0098 21504182 ssDNA20A8PGroup A Streptococcus (GAS)* 5'AGCAGCACAGAGGTCAGATGCCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCCCCTATGCGTGCTACCGTGAA3' 800.55Not reportedN/A5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2NoneTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableA total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000835
ABdb_0099 21504182 ssDNA20A9Group A Streptococcus (GAS)* 5'CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG3' 390.5128205128Kd: 13 ± 1 nM135′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Primer region removed (no primer version of 20a9)A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000836
ABdb_0100 21504182 ssDNA20A9PGroup A Streptococcus (GAS)* 5'AGCAGCACAGAGGTCAGATGCACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGGCCTATGCGTGCTACCGTGAA3' 790.5316455696Kd: 9.1 ± 0.8nM9.15′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableA total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000837
ABdb_0101 21504182 ssDNA20A12PGroup A Streptococcus (GAS)* 5'TTCACGGTAGCACGCATAGGGCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCCCATCTGACCTCTGTGCTGCT3' 780.5897435897Kd: 25 ± 3 nM255′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableA total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000838
ABdb_0102 21504182 ssDNA20A14PGroup A Streptococcus (GAS)* 5'AGCAGCACAGAGGTCAGATGGGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGCCCTATGCGTGCTACCGTGAA3' 800.5625Kd: 17 ± 1 nM175′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′401× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2MgClTris Buffers7.4Not reportedDiagnostic and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."Not applicableA total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition10000839
ABdb_0086 21627466 ssDNA19Enterotoxigenic Escherichia coli (E. coli) (ETEC) K88 fimbriae protein 5′CGTACGGTCGACGCTAGCGGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTCCACGTGGAGCTCGGATCC3′ 960.6458333333Kd: 44 ± 7 nM445′-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3′60(SHCMK) containing 20 mmol/L HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), pH 7.35; 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KClMgCl/CaClOther Buffers7.35Not reportedDiagnostic and Therapeutic: "In this study, the first group of single-stranded DNA aptamers that are highly specific to enterotoxigenic Escherichia coli (ETEC) K88 was obtained from an enriched oligonucleotide pool by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Since ETEC K88 is the only type of bacterium that expressed abundant K88 fimbriae, the selected aptamers against the K88 fimbriae protein were able to specifically identify ETEC K88 among other bacteria. This method of detecting ETEC K88 by aptamers can also be applied to bacteria other than ETEC K88."Not applicable10000847
ABdb_0087 21627466 ssDNA31Enterotoxigenic Escherichia coli (E. coli) (ETEC) K88 fimbriae protein 5′CGTACGGTCGACGCTAGCACACTCTTTTGCTCGTGTTTTTGCCTGTTACATAAAATGAATCAGTGGATGTTTCCTTCTCACGTGGAGCTCGGATCC3′ 960.4791666667Kd: 36 ± 8 nM365′-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3′60(SHCMK) containing 20 mmol/L HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), pH 7.35; 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KClMgCl/CaClOther Buffers7.35Not reportedDiagnostic and Therapeutic: "In this study, the first group of single-stranded DNA aptamers that are highly specific to enterotoxigenic Escherichia coli (ETEC) K88 was obtained from an enriched oligonucleotide pool by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Since ETEC K88 is the only type of bacterium that expressed abundant K88 fimbriae, the selected aptamers against the K88 fimbriae protein were able to specifically identify ETEC K88 among other bacteria. This method of detecting ETEC K88 by aptamers can also be applied to bacteria other than ETEC K88."Not applicable10000848
ABdb_0088 21627466 ssDNA7Enterotoxigenic Escherichia coli (E. coli) (ETEC) K88 fimbriae protein 5′CGTACGGTCGACGCTAGCGGCGGCCGTGAAATTTGCCAAATGCCGTCTTGGCTTTCGCCCAATGTATCCTGGGTGTTCCACGTGGAGCTCGGATCC3′ 960.59375Kd: 43 ± 6 nM435′-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3′60(SHCMK) containing 20 mmol/L HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), pH 7.35; 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KClMgCl/CaClOther Buffers7.35Not reportedDiagnostic and Therapeutic: "In this study, the first group of single-stranded DNA aptamers that are highly specific to enterotoxigenic Escherichia coli (ETEC) K88 was obtained from an enriched oligonucleotide pool by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Since ETEC K88 is the only type of bacterium that expressed abundant K88 fimbriae, the selected aptamers against the K88 fimbriae protein were able to specifically identify ETEC K88 among other bacteria. This method of detecting ETEC K88 by aptamers can also be applied to bacteria other than ETEC K88."Not applicable10000849
ABdb_0089 21627466 ssDNA37Enterotoxigenic Escherichia coli (E. coli) (ETEC) K88 fimbriae protein 5′CGTACGGTCGACGCTAGCGGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGCCACGTGGAGCTCGGATCC3′ 960.6041666667Kd: 25 ± 4 nM255′-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3′60(SHCMK) containing 20 mmol/L HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), pH 7.35; 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KClMgCl/CaClOther Buffers7.35Not reportedDiagnostic and Therapeutic: "In this study, the first group of single-stranded DNA aptamers that are highly specific to enterotoxigenic Escherichia coli (ETEC) K88 was obtained from an enriched oligonucleotide pool by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Since ETEC K88 is the only type of bacterium that expressed abundant K88 fimbriae, the selected aptamers against the K88 fimbriae protein were able to specifically identify ETEC K88 among other bacteria. This method of detecting ETEC K88 by aptamers can also be applied to bacteria other than ETEC K88."Not applicable10000850
ABdb_0090 22166202 ssRNATwo ArmEscherichia coli (E. coli) O157:H7 5'GGGAUACCAGCUUAUUCAAUUUGAUUCCAUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAAGUGCAAUCU3' 990.3838383838Kd: 110 nM1105′-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3′60200 ul (30 mM Tris-HCl, ph 7.5, 150 mM NaCl, 1.5 mM MgCl2, 2mM dithiothreitol, 1% BSA)MgClTris Buffers7.5Not reportedDiagnostic: " In this study, a RNA aptamer-based ligand which can specifically distinguish the pathogen E. coli O157:H7 from others was developed by a subtractive cell-SELEX method. This novel O157:H7-specific aptamer could be of potential application as a diagnostic ligand against the pathogen-related food borne illness."Truncated to: 5'-gggtcttccthhacthtchaaaattcagtatcgggaggttacgtatttggtttatagatagtaa-3'10000827
ABdb_0127 22480209 ssDNAA1PVibrio parahaemolyticus (V. parahaemolyticus) 5'ATAGGAGTCACGACGACCAGAATAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAGTATGTGCGTCTACCTCTTGACTAAT3′ 870.4827586207Kd: 21.45 ± 2.62 nM21.455′-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3′401× BB, 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2MgClTris Buffers7.4Not reportedDetection: " In this study, whole-bacterium SELEX was employed to identify DNA aptamer sequences specific to V. parahemolyticus that have the potential to be further developed into a rapid isolation/identification technology to facilitate the detection of V. parahemolyticus in food and environmental samples. Whole-bacterium SELEX is [also] a promising technique for the design of aptamer-based molecular probes for microbial pathogens that does not require the labor-intensive steps of isolating and purifying complex markers or targets."Not applicable10000851
ABdb_0125 22480209 ssDNAA3PVibrio parahaemolyticus (V. parahaemolyticus) 5'ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT3′ 870.4137931034Kd: 16.88 ± 1.92 nM16.885′-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3′401× BB, 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2MgClTris Buffers7.4Not reportedDetection: " In this study, whole-bacterium SELEX was employed to identify DNA aptamer sequences specific to V. parahemolyticus that have the potential to be further developed into a rapid isolation/identification technology to facilitate the detection of V. parahemolyticus in food and environmental samples. Whole-bacterium SELEX is [also] a promising technique for the design of aptamer-based molecular probes for microbial pathogens that does not require the labor-intensive steps of isolating and purifying complex markers or targets."Not applicable10000852285
ABdb_0197 23387511 ssDNASE-3 (80nt)Salmonella enterica (S. enteritidis) 5′CTCCTCTGACTGTAACCACGTCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC3′ 800.55Not reportedN/A5′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Not applicableaptamer can be used as affitity probe in flow cytometry or biosensors10000931
ABdb_0198 23387511 ssDNASE-3 (60nt)Salmonella enterica (S. enteritidis) 5'TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC3′ 600.55Kd: 7.8 ± 6.1 nM7.85′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the first 20 basesaptamer can be used as affitity probe in flow cytometry or biosensors10000932
ABdb_0199 23387511 ssDNASE-6 (79nt)Salmonella enterica (S. enteritidis) 5'CTCCTCTGACTGTAACGTACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC'3 710.4647887324Kd: 53 ± 7 nM (affinity for 80nt)535′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Not applicableaptamer has good antibacterial potential10000933
ABdb_0200 23387511 ssDNASE-6 (54nt)Salmonella enterica (S. enteritidis) 5'TACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC'3 540.4444444444Kd: 6.3 ± 0.58 nM (affinity for 60nt)6.35′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Truncated by removing the first 25 basesaptamer has good antibacterial potential10000934
ABdb_0201 23387511 ssDNASE-20 (80nt)Salmonella enterica (S. enteritidis) 5′CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGTGCATAGGTAGTCCAGAAGCC3′ 800.5375Kd: 28 ± 3.8 nM285′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Not applicableaptamer has good antibacterial potential10000935
ABdb_0202 23387511 ssDNASE-20 (60nt)Salmonella enterica (S. enteritidis) 5′CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT3' 600.5333333333Kd: 7.1 ± 0.62 nM7.15′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the last 20 basesaptamer has good antibacterial potential10000936
ABdb_0205 23387511 ssDNASE-11 (60nt)Salmonella enterica (S. enteritidis) 5′CTCCTCTGACTGTAACCACGAACGATTCAAGAACTGTTGGTTGTCGGCTTATTTTCGGCA3′ 600.4666666667Kd: 6.9 ± 0.4 nM6.95′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the last 20 basesaptamer can be used as affitity probe in flow cytometry or biosensors10000937
ABdb_0206 23387511 ssDNASE-34 (80nt)Salmonella enterica (S. enteritidis) 5′CTCCTCTGACTGTAACCACGTGCCGCTAAACGCCGGCTCATCGTTATGCTTTTCATTGCAGCATAGGTAGTCCAGAAGCC3′ 800.525Kd: 56 ± 7.1 nM565′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Not applicableaptamer can be used as affitity probe in flow cytometry or biosensors10000938
ABdb_0207 23387511 ssDNAST-1 (40nt)Salmonella typhimurium (S. typhimurium) 5′GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCC3′ 400.525Kd: 5.3 ± 0.4 nM5.35′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the first 20 bases and the last 20 basesaptamer can be used as affitity probe in flow cytometry or biosensors10000940
ABdb_0208 23387511 ssDNAST-6 (60nt)Salmonella typhimurium (S. typhimurium) 5′GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAAGCTGCTCGCATAGGTAGTCCAGAAGCC3′ 610.5901639344Kd: 13 ± 1.1 nM135′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the first 20 basesaptamer can be used as affitity probe in flow cytometry or biosensors10000941
ABdb_0209 23387511 ssDNAST-12 (80nt)Salmonella typhimurium (S. typhimurium) 5′CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTCGCATAGGTAGTCCAGAAGCC3′ 800.5125Kd: 15.4 ±1.8 nM15.45′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Not applicableaptamer has good antibacterial potential10000942
ABdb_0210 23387511 ssDNAST-12 (60nt)Salmonella typhimurium (S. typhimurium) 5′CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT3′ 590.4915254237Kd: 4.5 ± 0.4 nM4.55′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the last 20 basesaptamer has good antibacterial potential10000943
ABdb_0213 23387511 ssDNAST-33 (60nt)Salmonella typhimurium (S. typhimurium) 5′CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG3′ 600.5166666667Kd: 51 ± 4.3 nM515′-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3′40500 μl DPBS bufferNonePBS/phosphate buffers7.4Not reportedTherapeutic: " Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential."Trucated by removing the last 20 basesaptamer has good antibacterial potential10000944
ABdb_0232 23698275 ssDNAApt22Salmonella Paratyphi A 5'GAATTCAGTCGGACAGCGATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGCGGATGGACGAATATCGTCTCCC3' 800.525Kd: 47 ± 3 nM475′-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3′4050 mM Tris-HCl (pH 7.4), 100 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 0.1% NaN3MgClTris Buffers7.4Not reportedDetection: " In this paper, a panel of single-stranded DNA aptamers with high affinity and specificity against Salmonella Paratyphi A was selected from an enriched oligonucleotide pool by a whole-cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure. This study demonstrated the applicability of Salmonella specific aptamers and their potential for use in the detection of Salmonella in food, clinical and environmental samples."Not applicableUnclear if the primer/static-regions are part of the aptamer. This UTexas Aptamer DB includes them as part of the aptamer, but the Apta-Index does not.The FITC-labeled forward primer (5'-C12-FITC-GAATTCAGTCGGACAGCG-3') and biotin-labeled reverse primer (5'-Bio-GGGAGACGATATTCGTCCATC-3') were used in PCR to get the double-labeled DNA and measure the concentration of the ssDNA in fluorescence analysis.100009687272
ABdb_0250 23942378 ssDNALbi-17Listeria (L. monocytogenes) 5′AGTATACGTATTACCTGCAGCGAGGGAAGAAGGGCCAGCACAGATCAGATCAATCGCTCCGCGATATCTCGGAGATCTTGC3′ 810.5185185185Kd: 35.7 ± 8.02 μM357005′-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3′401xPBST, 0.05%Tween20 in 1xPBSNonePBS/phosphate buffers7.4Not reportedDiagnostic and Detection: " The purpose of this study was to identify biotinylated single-stranded (ss) DNA aptamers with binding specificity to Listeria and use these for capture and subsequent qPCR detection of the organism. Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods."Not applicable10000919
ABdb_0251 23942378 ssDNALbi-16Listeria (L. monocytogenes) 5′AGTATACGTATTACCTGCAGCAAATACTTTAGATCTAAGAGTGTTTCGAAAAGACAACAGACGATATCTCGGAGATCTTGC3′ 810.3827160494Not reportedN/A5′-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3′401xPBST, 0.05%Tween20 in 1xPBSNonePBS/phosphate buffers7.4Not reportedDiagnostic and Detection: " The purpose of this study was to identify biotinylated single-stranded (ss) DNA aptamers with binding specificity to Listeria and use these for capture and subsequent qPCR detection of the organism. Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods."Not applicable10000920
ABdb_0252 23942378 ssDNALbi-118Listeria (L. monocytogenes) 5′AGTATACGTATTACCTGCAGCTTGAATTAATAGATTAGTATACTTGGGAATCGTCCTAATACGATATCTCGGAGATCTTGC3′ 810.3703703704Not reportedN/A5′-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3′401xPBST, 0.05%Tween20 in 1xPBSNonePBS/phosphate buffers7.4Not reportedDiagnostic and Detection: " The purpose of this study was to identify biotinylated single-stranded (ss) DNA aptamers with binding specificity to Listeria and use these for capture and subsequent qPCR detection of the organism. Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods."Not applicable10000921
ABdb_0253 23942378 ssDNALbi-200Listeria (L. monocytogenes) 5′AGTATACGTATTACCTGCAGCAGGAAGACAAATTCCGCCAAAAAGTGGATATAACCAATAACGATATCTCGGAGATCTTGC3′ 810.4074074074Not reportedN/A5′-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3′401xPBST, 0.05%Tween20 in 1xPBSNonePBS/phosphate buffers7.4Not reportedDiagnostic and Detection: " The purpose of this study was to identify biotinylated single-stranded (ss) DNA aptamers with binding specificity to Listeria and use these for capture and subsequent qPCR detection of the organism. Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods."Not applicable10000922
ABdb_0254 23942378 ssDNALbi-203Listeria (L. monocytogenes) 5′AGTATACGTATTACCTGCAGCATAGAGTAGAAGCTACACTACGTAATCACAGACAGATCCACGATATCTCGGAGATCTTGC3′ 810.4320987654Not reportedN/A5′-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3′401xPBST, 0.05%Tween20 in 1xPBSNonePBS/phosphate buffers7.4Not reportedDiagnostic and Detection: " The purpose of this study was to identify biotinylated single-stranded (ss) DNA aptamers with binding specificity to Listeria and use these for capture and subsequent qPCR detection of the organism. Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods."Not applicable10000923
ABdb_0220 23978634 ssDNAC4Salmonella Typhimurium 5′CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGTACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCGGGTGGATCCATATTCCTACTCG3′ 1050.580952381Not reportedN/A5′-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3′4050 μl PBS (pH 7.4, Sigma, MO, USA)NonePBS/phosphate buffers7.4Not reportedDetection: " In this study, aptamer against S. Typhimurium was selected through the whole-cell SELEX technique. Whole-cell SELEX is considered to be a useful aptamer selection tool, as aptamers derived in this way can target whole live bacteria. Our modified strategy has improved the development of aptamers against bacteria. Furthermore, aptamer C4 could be a potential ligand for capturing S. Typhimurium, and be useful in development of easy, rapid, and sensitive methodology for pathogen detection."Not appilcableThe selected DNA was fluorescently labeled via PCR amplification with 5′-FAM (fluorescein)- and 3′-FAM-modified primers10000897
ABdb_0238 25436184 ssDNADTMRSA1Methicillin-resistant Staphylococcus aureus (MRSA) 5'ATCCAGAGTGACGCAGCAATCCAGACGTGACGCAGCATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTGTGGACACGGTGGCTTAGTATGGACACGGTGGCTTAGT3' 1110.5405405405kD: 160 ± 50 nM1505'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'404.5 g/L glucose, 5 mL MgCl2, 1.0 g/L bovine serum albumin and 100 mg/L tRNAMgClOther Buffers7.4Not reportedTherapeutic: " The Cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology was used to run the selection against MRSA bacteria and develop target-specific aptamers. The development of MRSA aptamer would present a great tool to new therapeutic approaches to eradicate the MRSA superbug, either without the use of antibiotics or with a strain-specific antibiotic."Not applicable10000967
ABdb_0506 26541162 ssDNAMtb36Mycobacterium tuberculosis (M. tuberculosis) H37Ra 5′CCGTCAGCCGAGGACCACACTTGGTTGCTGAATCCCCTCGTCTTGGCTTTCTTTTGGGTGCAATGAAT3′ 680.5294117647Kd: 5.09 ±1.43 nM5.095′-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3′40PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4) and PBST (0.05 % Tween 20 in PBS)NonePBS/phosphate buffers7.4Not reportedBiosensor and Diagnostic: "Tuberculosis (TB) remains to be a major global health problem, with about 9 million new cases and 1.4 million deaths in 2011. Aptamers are promising tools for developing point-of-care diagnostic assays for TB. In this study, ssDNA aptamers that recognize Mycobacterium tuberculosis H37Ra were selected by systematic evolution of ligands by exponential enrichment (SELEX). Mtb36 aptamer is highly selective for M. tuberculosis, and it can be used in an aptamer-based biosensor for the detection of M. tuberculosis."Not applicable10001059
ABdb_0559 26678795 ssDNAE-CA 20M proteins on the surface of Streptococcus pyogenes (S. pyogenes) 5′CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG3′ 400.575Kd: 7±1 nM75'-TTCACGGTAGCACGCATAGG-N40-CATCTGACCTCTGTGCTGCT-3'4010 mM Tris buffer PH 8 @ (21 °C) for 60 minNoneTris Buffers8Not reportedDetection: " The primary objective of this work is to develop a technique that enables generation of aptamers binding to specific M-types of S. pyogenes. The improved bacterial cell-SELEX technique is successful in generating aptamers selective for S. pyogenes and some of its M-types. These aptamers are potentially useful for detecting S. pyogenes, achieving binding profiles of the various M-types, and developing new M-typing technologies for non-specialized laboratories or point-of-care testing."Primer region removed for this aptamer10001035
ABdb_0560 26678795 ssDNAE-CA 20PM proteins on the surface of Streptococcus pyogenes (S. pyogenes) 5′TTCACGGTAGCACGCATAGGCACACACGGAACCCCGACAACATACATACGGTGAGGGTGGCATCTGACCTCTGTGCTGCT3′ 800.5625Kd: 12 ± 1 nM125'-TTCACGGTAGCACGCATAGG-N40-CATCTGACCTCTGTGCTGCT-3'4010 mM Tris buffer PH 8 @ (21 °C) for 60 minNoneTris Buffers8Not reportedDetection: " The primary objective of this work is to develop a technique that enables generation of aptamers binding to specific M-types of S. pyogenes. The improved bacterial cell-SELEX technique is successful in generating aptamers selective for S. pyogenes and some of its M-types. These aptamers are potentially useful for detecting S. pyogenes, achieving binding profiles of the various M-types, and developing new M-typing technologies for non-specialized laboratories or point-of-care testing."Not applicable10001036
ABdb_0561 26678795 ssDNAD-Cells 9M proteins on the surface of Streptococcus pyogenes (S. pyogenes) 5′GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG3′ 400.55Kd: 71 ± 23 nM715'-TTCACGGTAGCACGCATAGG-N40-CATCTGACCTCTGTGCTGCT-3'4010 mM Tris buffer PH 8 @ (21 °C) for 60 minNoneTris Buffers8Not reportedDetection: " The primary objective of this work is to develop a technique that enables generation of aptamers binding to specific M-types of S. pyogenes. The improved bacterial cell-SELEX technique is successful in generating aptamers selective for S. pyogenes and some of its M-types. These aptamers are potentially useful for detecting S. pyogenes, achieving binding profiles of the various M-types, and developing new M-typing technologies for non-specialized laboratories or point-of-care testing."Primer region removed for this aptamer10001037
ABdb_0562 26678795 ssDNAD-Cells 9PM proteins on the surface of Streptococcus pyogenes (S. pyogenes) 5′TTCACGGTAGCACGCATAGGGGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGGCATCTGACCTCTGTGCTGCT3′ 800.55Kd: 44 ± 8 nM445'-TTCACGGTAGCACGCATAGG-N40-CATCTGACCTCTGTGCTGCT-3'4010 mM Tris buffer PH 8 @ (21 °C) for 60 minNoneTris Buffers8Not reportedDetection: " The primary objective of this work is to develop a technique that enables generation of aptamers binding to specific M-types of S. pyogenes. The improved bacterial cell-SELEX technique is successful in generating aptamers selective for S. pyogenes and some of its M-types. These aptamers are potentially useful for detecting S. pyogenes, achieving binding profiles of the various M-types, and developing new M-typing technologies for non-specialized laboratories or point-of-care testing."Not applicable10001038
ABdb_0564 26678795 ssDNAE-Cells 1PM proteins on the surface of Streptococcus pyogenes (S. pyogenes) 5′TTCACGGTAGCACGCATAGGGGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGGCATCTGACCTCTGTGCTGCT3′ 790.5569620253Kd: 20 ± 3 nM205'-TTCACGGTAGCACGCATAGG-N40-CATCTGACCTCTGTGCTGCT-3'4010 mM Tris buffer PH 8 @ (21 °C) for 60 minNoneTris Buffers8Not reportedDetection: " The primary objective of this work is to develop a technique that enables generation of aptamers binding to specific M-types of S. pyogenes. The improved bacterial cell-SELEX technique is successful in generating aptamers selective for S. pyogenes and some of its M-types. These aptamers are potentially useful for detecting S. pyogenes, achieving binding profiles of the various M-types, and developing new M-typing technologies for non-specialized laboratories or point-of-care testing."Not applicable10001039
ABdb_0764 27104834 ssDNAP12-17Escherichia coli (E. coli) ATCC 25922 5'CATACGATTTAGGTGACACTATAGGACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGGATTTCTCCTACTGGGATAGGTGGA3' 880.5454545455Kd: 56.33 ± 15.87 nM56.335'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'40PBS containing 1.4 mM MgCl2MgClPBS/phosphate buffers7.4Not reportedDiagnostic: " Escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. We used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to isolate four single stranded DNA (ssDNA) aptamers that bind strongly to E. coli cells (ATCC generic strain 25922), with Kd values in the nanomolar range. This aptamer binds to Meningitis/sepsis associated E. coli (MNEC) clinical isolates, and is the first aptamer described with potential for use in the diagnosis of MNEC-borne pathologies."Not applicable10001175
ABdb_0762 27104834 ssDNAP12-31Escherichia coli (E. coli) ATCC 25922 5'CATACGATTTAGGTGACACTATAGTCATCGGGATACCTGGTAAGGATAATTTCTCCTACTGGGATAGGTGGA3' 720.4305555556Kd: 87.03 ± 17.32 nM87.035'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'40PBS containing 1.4 mM MgCl2MgClPBS/phosphate buffers7.4Not reportedDiagnostic: " Escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. We used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to isolate four single stranded DNA (ssDNA) aptamers that bind strongly to E. coli cells (ATCC generic strain 25922), with Kd values in the nanomolar range. This aptamer binds to Meningitis/sepsis associated E. coli (MNEC) clinical isolates, and is the first aptamer described with potential for use in the diagnosis of MNEC-borne pathologies."Not applicable10001176
ABdb_0761 27104834 ssDNAP12-52Escherichia coli (E. coli) ATCC 25922 5'CATACGATTTAGGTGACACTATAGCCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCGATTTCTCCTACTGGGATAGGTGGA3' 880.5795454545Kd:11.97 ± 2.94 nM11.975'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'40PBS containing 1.4 mM MgCl2MgClPBS/phosphate buffers7.4Not reportedDiagnostic: " Escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. We used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to isolate four single stranded DNA (ssDNA) aptamers that bind strongly to E. coli cells (ATCC generic strain 25922), with Kd values in the nanomolar range. This aptamer binds to Meningitis/sepsis associated E. coli (MNEC) clinical isolates, and is the first aptamer described with potential for use in the diagnosis of MNEC-borne pathologies."Not applicable10001177
ABdb_0763 27104834 ssDNAP12-55Escherichia coli (E. coli) ATCC 25922 5'CATACGATTTAGGTGACACTATAGCCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGAATTTCTCCTACTGGGATAGGTGGA3' 1280.6015625Kd: 161.0 ± 34.74 nM1615'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'40PBS containing 1.4 mM MgCl2MgClPBS/phosphate buffers7.4Not reportedDiagnostic: " Escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. We used cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) to isolate four single stranded DNA (ssDNA) aptamers that bind strongly to E. coli cells (ATCC generic strain 25922), with Kd values in the nanomolar range. This aptamer binds to Meningitis/sepsis associated E. coli (MNEC) clinical isolates, and is the first aptamer described with potential for use in the diagnosis of MNEC-borne pathologies."Not applicable10001178
ABdb_0755 27529508 ssDNABM4Mannose-capped lipoarabinomannan (ManLAM) of Bacillus Calmette–Guérin (BCG) 5'GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCCCGCGCAGCGGGTCGACTTCATTCCTCACCATGGGTCAATGCGTCATA3' 890.5393258427Not reportedN/A5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'3120 mM Tris-HCl, 0.15 M NaCl, 10 mM CaCl2, 6 mM MnCl2, pH 7.2 at 37 ˚CNoneTris Buffers7.272–95 kDaTheruputic: " Because Mycobacterium bovis, termed bacillus Calmette-Guérin (BCG), the only available used tuberculosis (TB) vaccine, retains immunomodulatory properties that limit its protective immunogenicity, there are continuous efforts to identify the immunosuppression mechanism as well as new strategies for improving the immunogenicity of BCG. Here, an ssDNA aptamer "antibody" BM2 specifically bound to the mannose-capped lipoarabinomannan (ManLAM) of BCG was selected. We report a new mechanism of the interaction between ManLAM and CD44 on macrophages and CD4(+) T cells and reveal that ManLAM-binding membrane molecule CD44 is a novel target for the enhancement of BCG immunogenicity, and BM2 has strong potential as an immune enhancer for BCG."Not applicable10001172
ABdb_0753 27529508 ssDNABM2Mannose-capped lipoarabinomannan (ManLAM) of Bacillus Calmette–Guérin (BCG) 5'GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCCCCCCATGAACTAGGCTCCACAATGAGTTTGGGGGTCAATGCGTCATA3' 890.5056179775Kd: 8.59 ± 1.23 nM8.595'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'3120 mM Tris-HCl, 0.15 M NaCl, 10 mM CaCl2, 6 mM MnCl2, pH 7.2 at 37 ˚CCaClTris Buffers7.272–95 kDaTheruputic: " Because Mycobacterium bovis, termed bacillus Calmette-Guérin (BCG), the only available used tuberculosis (TB) vaccine, retains immunomodulatory properties that limit its protective immunogenicity, there are continuous efforts to identify the immunosuppression mechanism as well as new strategies for improving the immunogenicity of BCG. Here, an ssDNA aptamer "antibody" BM2 specifically bound to the mannose-capped lipoarabinomannan (ManLAM) of BCG was selected. We report a new mechanism of the interaction between ManLAM and CD44 on macrophages and CD4(+) T cells and reveal that ManLAM-binding membrane molecule CD44 is a novel target for the enhancement of BCG immunogenicity, and BM2 has strong potential as an immune enhancer for BCG."Not applicable10001173
ABdb_0777 27871171 2'-fluoro-RNAEc3Escherichia coli (E.coli) DH5 alpha (DH5α) 5'AUACCAGCUUAUUCAAUUGCACGAAUUUGCUGUGUUUUUGGGGGGGUCGGGGAGUAUAAGAUAGUAAGUGCAAUCU3' 760.4210526316Kd: 225 nM2255'-AUACCAGCUUAUUCAAUU-N40-AGAUAGUAAGUGCAAUCU-3'4050 mM Tris pH7.4, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, 0.02% Tween 20, 0.1 mg/ml yeast tRNA and 1 mg/ml BSAMgClTris Buffers7.4Not reportedDetection: " Escherichia coli are important indicator organisms, used routinely for the monitoring of water and food safety. For quick, sensitive and real-time detection of E. coli we developed a 2'F modified RNA aptamer Ec3, by Cell-SELEX. We believe that our developed method can step towards more complex and real sample application."Not applicable2′F modified RNA aptamers10001138
ABdb_0746 27979272 ssDNAB5Salmonella typhimurium (S. typhimurium) 5'CAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT3' 900.5111111111Kd: 58.5 nM58.55′-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'4550 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2MgClTris Buffers7.5Not reportedDiagnostic and Detection: " In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. A QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium."5′-amino modification and a six carbon spacer. Then amino-modified aptamer was immobilized on the electrode surface for label-free detection of S. typhimurium.10001148
ABdb_0747 27979272 ssDNAA24Salmonella typhimurium (S. typhimurium) 5'CAGTCCAGGACAGATTCGCGAGGCACAATCCACCTCTCACCGCACGCCACGCACTGCCTCTGTCCCGCACGTGGATTTCATTCAGCGATT3' 900.5888888889Kd: 19.63 nM19.635′-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'4551 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2MgClTris Buffers7.5Not reportedDiagnostic and Detection: " In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. A QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium."5′-amino modification and a six carbon spacer. Then amino-modified aptamer was immobilized on the electrode surface for label-free detection of S. typhimurium.10001149
ABdb_0748 27979272 ssDNAB30Salmonella typhimurium (S. typhimurium) 5'AATCGCTGAATGAAATCCACGTGGGAAGTGTGTGGGTGACCAGAGGTGTGGTGATGGGATTGTCGTACCTCGCGAATCTGTCCTGGACTG3' 900.5333333333Kd: 51.02 nM51.025'-AATCGCTGAATGAAATCCACGTG-N45-CCTCGCGAATCTGTCCTGGACTG-3'4552 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2MgClTris Buffers7.5Not reportedDiagnostic and Detection: " In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. A QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium."5′-amino modification and a six carbon spacer. Then amino-modified aptamer was immobilized on the electrode surface for label-free detection of S. typhimurium.10001150
ABdb_0851 28441340 ssDNACCFM641-5Bifidobacterium bifidum (B. bifidum) 5'AGCAGCACAGAGGTCAGATGTGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGCCCTATGCGTGCTACCGTGAA3' 800.6125Kd: 10.69 ± 0.89 nM10.695'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'40Phosphate-buffere saline (PBS, pH 7.2)NonePBS/phosphate buffers7.2Not reportedDetection: " A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. To prove the potential application of the aptamer CCFM641-5, a colorimetric bioassay of the sandwich-type structure was used to detect B. bifidum. The assay had a linear range of 10⁴ to 10⁷ cfu/mL (R² = 0.9834). Therefore, the colorimetric bioassay appears to be a promising method for the detection of B. bifidum based on the aptamer CCFM641-5."Not applicableutilized whole-bacterium-based SELEX10001182
ABdb_0897 28513559 ssDNASS-3Shigella sonnei (S. sonnei) 5'ATACCAGCTTATTCAATTCCATGGTCCCTCGTGTTTATTATGTTGTCTGAACTGGCTGAGATTGCACTTACTATCT3' 760.3947368421Kd: 39.32 ± 5.02 nM39.325′-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′40TBS buffer (10 mM Tris-HCl, 0.85% NaCl, pH 8.0)NoneTris Buffers8.0Not reportedDetection: " In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. In this study, we demonstrated the feasibility of an aptamer sensor platform to detect S. sonnei in a variety of foods and pave the way for its use in diagnosing shigellosis through multiple, portable designs."Not applicableCy5 for detection probe at the 5' end; 5′-biotin-modified10001192
ABdb_0898 28513559 ssDNASS-4Shigella sonnei (S. sonnei) 5'ATACCAGCTTATTCAATTCCACACATACCAAAAACACAGCACACTTCATCAATTTCACGAGATTGCACTTACTATCT3' 770.3636363636Kd: 15.89 ± 1.77 nM15.895′-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′40TBS buffer (10 mM Tris-HCl, 0.85% NaCl, pH 8.0)NoneTris Buffers8.0Not reportedDetection: " In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. In this study, we demonstrated the feasibility of an aptamer sensor platform to detect S. sonnei in a variety of foods and pave the way for its use in diagnosing shigellosis through multiple, portable designs."5' amine for capture probe5′-biotin-modified10001193
ABdb_0827 28646719 ssDNACrn-1Salmonella enteritidis (S. enteritidis) 5'AAGGGCTGGCTGGGATGGACCCTCCCGAAACGAGCTGTCTCTTAACGGAAGCTAATCTGCCTCACTCCACGGACCCCACT3' 800.5875Kd: 971 nM9715′-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3′42Binding buffer (4.5 g/L glucose, 5mM MgCl2, 0.1mg/mL yeast tRNA, and 1mg/mL BSA in Dulbecco's PBSMgClPBS/phosphate buffers7.4not reportedDetection: " Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX). This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care."Not applicable10001190
ABdb_0828 28646719 ssDNACrn-2Salmonella enteritidis (S. enteritidis) 5'AAGGGCTGGCTGGGATGGATGTAAGAAGGGAGGAAAGGACCTAAGACCTGCTATATTGCGATCACTCCACGGACCCCACT3' 800.5375Kd: 309 nM3095′-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3′42Binding buffer (4.5 g/L glucose, 5mM MgCl2, 0.1mg/mL yeast tRNA, and 1mg/mL BSA in Dulbecco's PBSMgClPBS/phosphate buffers7.4Not reportedDetection: " Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX). This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care."Not applicable10001191
ABdb_1016 29132030 ssDNARAB1Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACGGGTGGGCTCCAATATGAATCGCTTGCCCTGACGCTATCTGCATAGTTAAGCCAGCC3' 760.5263157895Kd: 56 ± 87 nmol/mL565`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001199
ABdb_1017 29132030 ssDNARAB3Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACCGTAGTCTAGTGTCGATTAGTTTCCTTGAGACCTTGTGCTGCATAGTTAAGCCAGCC3' 760.4736842105Kd: 37 ± 112 nmol/mL375`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001200
ABdb_1018 29132030 ssDNARAB5Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACCGTAGTCTAGTGTCGATTAGTTTCCTTGCTATTGCAGACCTTGTGCTGCATAGTTAAGCCAGCC3' 830.4698795181Kd: 58 ± 14 nmol/mL585`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001201
ABdb_1012 29132030 ssDNARAB10Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACTCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCATGCATAGTTAAGCCAGCC3' 760.5263157895Kd: 46 ± 24 nmol/mL465`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001202
ABdb_1013 29132030 ssDNARAB20Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACGCGTTACGTTAGTGGCCGCCTATGAGGACAGGCGGTTGTAGCATAGTTAAGCCAGCC3' 760.5394736842Kd: 128 ± 45 nmol/mL1285`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001203
ABdb_1014 29132030 ssDNARAB28Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACTGGACGTCGTGGCGGAGGTTTTATAAAACGGCGCCACTGTGCATAGTTAAGCCAGCC3' 760.5263157895Kd: 49 ± 39 nmol/mL495`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001204
ABdb_1015 29132030 ssDNARAB35Staphylococcus aureus (S. aureus) surface molecules 5'TAGCTCACTCATTAGGCACGGGGGGTTGTGCCATTTAAGATGACCGGTTGCCGCGATTTGCATAGTTAAGCCAGCC3' 760.5263157895Kd: 34 ± 5 nmol/mL345`-TAGCTCACTCATTAGGCAC-N40-GCATAGTTAAGCCAGCC-3`40Binding buffer (BB) containing bovine serum albumin (BSA)NoneOther Buffers8Not reportedDetection: " In the present study, a high throughput whole cell SELEX method has been applied successfully in selecting specific aptamers against whole cells of Staphylococcus aureus, a potent food poisoning bacterium. The established assay could be a reliable detection tool for the routine investigation of Staphylococcus aureus in samples from food and clinical sources."Not applicable10001205
ABdb_1022 29580944 ssDNAML12Lethal factor (LF): one of three toxins produced by Bacillus anthracis (B. anthracis) 5′GCGCGGATCCCGCGCCGAGGGAGACGCGAACCTTCTCGCCTTGGGCGCGCGAAGCTTGCG3′ 600.7333333333Kd: 11.0 ± 2.7 nM via gel shift assay & 530 ± 11 nM via nonlinear fitting of the saturation binding curve from ELISA. IC50: 15 ± 1.5 μM115′-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3′3030 mM Tris-HCl at pH 8.4, 3.0 mM KCl, 600 mM NaCl, and 0.6 mM MgCl2MgClTris Buffers8.4Not reportedDetection and Therapeutic: " Anthrax is caused by Bacillus anthracis, a bacterium that is able to secrete the toxins protective antigen, edema factor and lethal factor. Due to the high level of secretion from the bacteria and its severe virulence, lethal factor (LF) has been sought as a biomarker for detecting bacterial infection and as an effective target to neutralize toxicity. In this study, we found three aptamers, and binding affinity was determined by fluorescently labeled aptamers. This aptamer provides a potential clue for not only development of a sensitive diagnostic device of B. anthracis infection but also the design of novel inhibitors of LF."Not applicable10001223
ABdb_0830 29594592 ssDNAApt22Antigen 85 (Ag85A) (FbpA) 5'GCTGTGTGACTCCTGCAAGCGGGAAGAGGGTAAGGGGAGGGAGGGTAACGCGGAGAAGGCAAGCAGCTGTATCTTGTCTCC3' 810.5925925926Kd: 62.95 nM62.955′-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3′43PBS-T containing 1 mg.mL−1 bovine serum albumin (BSA)NonePBS/phosphate buffersN/ANot reportedDiagnostic: " The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. On the basis of its performance, the GO-based fluorescent aptasensor revealed a promising future for the detection of protein biomarkers of M. tuberculosis"Not applicable10001197
ABdb_0831 29594592 ssDNAApt8Antigen 85 (Ag85A) (FbpA) 5'GCTGTGTGACTCCTGCAATCAGGAAAGAACTTAGGGGTGGGAGGAGGGTATAAATACGGAATGCAGCTGTATCTTGTCTCC3' 810.4938271605Kd: 202 nM2025′-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3′43PBS-T containing 1 mg.mL−1 bovine serum albumin (BSA)NonePBS/phosphate buffersN/ANot reportedDiagnostic: " The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. On the basis of its performance, the GO-based fluorescent aptasensor revealed a promising future for the detection of protein biomarkers of M. tuberculosis"Not applicable10001198
ABdb_1003 30205966 ssDNAH63Mycobacterium tuberculosis HspX antigen 5′GTCTTGACTAGTTACGCCGGGAACAATATGTTCAAGGGCTCTTTAAAGTTTTAGTTCGTTTGTCATTCAGTTGGCGCCTC3′ 800.4375Kd: 371 ±  0.02 nM3715′-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3′4410 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaClMgClTris Buffers7.5Not reportedDetection: " Herein, we report the selection and characterization of a panel of high affinity ssDNA aptamers against Mycobacterium tuberculosis (Mtb) HspX antigen by combining subtractive SELEX technology with biophysical characterization. An in silico secondary structure-guided approach of post-SELEX optimization through aptamer truncation and mutation studies led to the selection and evolution of aptamer H63 and its variant (H63 SL-2 M6), which demonstrated a high degree of accuracy as a diagnostic tool for TBM and has potential for translation into a rapid format test for TBM using CSF specimens."Not applicableN/A10001262
ABdb_1004 30205966 ssDNAH63 SL-2 M6Mycobacterium tuberculosis HspX antigen 5′GTCTTGACTAGTTACGCCGGGAACAATATGTTCAAGGGCTTTTTTTTTTTTTAGTTCGTTTGTCATTCAGTTGGCGCCTC3′ 800.4125Kd: 90 ± 2E-5 nM905′-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3′4410 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaClMgClTris Buffers7.5Not reportedDetection: "Herein, we report the selection and characterization of a panel of high affinity ssDNA aptamers against Mycobacterium tuberculosis (Mtb) HspX antigen by combining subtractive SELEX technology with biophysical characterization. An in silico secondary structure-guided approach of post-SELEX optimization through aptamer truncation and mutation studies led to the selection and evolution of aptamer H63 and its variant (H63 SL-2 M6), which demonstrated a high degree of accuracy as a diagnostic tool for TBM and has potential for translation into a rapid format test for TBM using CSF specimens."Truncated (28-mer) and mutated derived from aptamer h63N/A10001263
ABdb_1049 30685034 ssDNAApt-1Vibrio parahaemolyticus (V. parahaemolyticus) 5'TCAGCACGTAGCCCTATTTGTTTTCTCTTGTCTCTGTACTGCTGATGTTGGTCATTCTCTTTTCTCGGTTCCACTGAGAGATCC3' 840.4523809524Kd: 19.2 ± 2.8 nM19.25'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'60Binding Buffer (1X BB): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 4 mg/L BSA, 0.05% v/v Tween-20.NonePBS/phosphate buffersN/ANot reportedBiosensor: " Here, we developed an advanced cell-SELEX strategy featuring functionalized graphene oxide (GO) and isothermal rolling circle amplification (RCA) to select aptamers against a prevailing foodborne pathogen, Vibrio parahaemolyticus. Simple procedure, high efficiency, and free from expensive thermal cycler (required by PCR amplification) will enable the established strategy to find its applications in aptamer selecting against fungi, stem and cancerous cells as well."Not applicable10001237
ABdb_1048 30685034 ssDNAApt-2Vibrio parahaemolyticus (V. parahaemolyticus) 5'TCAGCACGTATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGCTCCACTGAGAGATCC3' 840.4047619048Kd: 10.3 ± 4.5 nM10.35'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'60Binding Buffer (1X BB): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 4 mg/L BSA, 0.05% v/v Tween-20.NonePBS/phosphate buffersN/ANot reportedBiosensor: " Here, we developed an advanced cell-SELEX strategy featuring functionalized graphene oxide (GO) and isothermal rolling circle amplification (RCA) to select aptamers against a prevailing foodborne pathogen, Vibrio parahaemolyticus. Simple procedure, high efficiency, and free from expensive thermal cycler (required by PCR amplification) will enable the established strategy to find its applications in aptamer selecting against fungi, stem and cancerous cells as well."Not applicable10001238
ABdb_1050 30685034 ssDNAApt-3Vibrio parahaemolyticus (V. parahaemolyticus) 5'TCAGCACGTTATCTAGTCAGATATCCAGACAGTCGCGGCTGGAAGCTTCTGTTAAGAATTTGAGATACTTCCACTGAGAGATCC3' 840.4523809524Kd: 25.2 ± 3.1 nM25.25'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'60Binding Buffer (1X BB): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 4 mg/L BSA, 0.05% v/v Tween-20.NonePBS/phosphate buffersN/ANot reportedBiosensor: " Here, we developed an advanced cell-SELEX strategy featuring functionalized graphene oxide (GO) and isothermal rolling circle amplification (RCA) to select aptamers against a prevailing foodborne pathogen, Vibrio parahaemolyticus. Simple procedure, high efficiency, and free from expensive thermal cycler (required by PCR amplification) will enable the established strategy to find its applications in aptamer selecting against fungi, stem and cancerous cells as well."Not applicable10001239
ABdb_1051 30685034 ssDNAApt-4Vibrio parahaemolyticus (V. parahaemolyticus) 5'TCAGCACGTGCGGGCATTATTGCACTCCACGGCGAGTAGTGCTCACAGAGTCATTTACACCGGTCGTATTCCACTGAGAGATCC3' 840.5357142857Kd: 17.2 ± 5.1 nM17.25'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'60Binding Buffer (1X BB): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 4 mg/L BSA, 0.05% v/v Tween-20.NonePBS/phosphate buffersN/ANot reportedBiosensor: " Here, we developed an advanced cell-SELEX strategy featuring functionalized graphene oxide (GO) and isothermal rolling circle amplification (RCA) to select aptamers against a prevailing foodborne pathogen, Vibrio parahaemolyticus. Simple procedure, high efficiency, and free from expensive thermal cycler (required by PCR amplification) will enable the established strategy to find its applications in aptamer selecting against fungi, stem and cancerous cells as well."Not applicable10001240
ABdb_1218 31704587 ssDNAMS10Mycobacterium tuberculosis (Mtb) Malate synthaseMS 5'GTCTTGACTAGTTACGCCGGTGTGTTGACTGAGGGGGTGGGGTGGGTGGTGGTGGATATAGCTCATTCAGTTGGCGCCTC3' 800.575Kd: 12 nM125'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'4410 mM Tris [pH 7.5], 10 mM MgCl2, 50 mM KCl, 25 mM NaCl)MgClTris Buffers7.5Not reportedDetection: " The theranostic potential of the best-performing and optimized G4-forming aptamer, MS10-Trunc, was established through binding studies using ALISA and SPR, enzyme inhibition assay, binding and inhibition of Mtb invasion into host cells, and demonstrating its diagnostic utility in TBM. We anticipate that MS10-Trunc holds promise for the development of a novel MS-targeted theranostics approach against Mtb and may complement the existing diagnostic and therapeutic modalities in the near future."Not appilcablePool was obtained from a cited paper: Dhiman, A., Haldar, S., Mishra, S. K., Sharma, N., Bansal, A., Ahmad, Y., Kumar, A., Sharma, T. K., & Tyagi, J. S. (2018). Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis. Tuberculosis (Edinburgh, Scotland), 112, 27–36. https://doi.org/10.1016/j.tube.2018.07.00410001309
ABdb_1349 31953175 ssDNAApt17SipA effector protein secreted by type three secretory system (T3SS) 5′TAGGGAAGAGAAGGACATATGATGCAATGGAACCGCTGAACGACCCTAGCATTATCAGTGTGGTTGACTAGTACATGACCACTTGA3′ 860.4534883721Kd: 114.9 nM at 27 °C and 63.4 nM at 37 °C114.95′-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGT ACATGACCACTTGA-3′40Binding buffer (50 mM Tris-HCl, 100 mM NaCl, 5 mM KCl and 1 mM MgCl2, pH 7.4)MgClTris Buffers7.4~50 kDa in sds-pageTherapeutic and Diagnostic: " Salmonella Enteritidis is an important pathogen that can invade the intestinal cells of its host causing salmonellosis. SipA protein, an effector protein secreted by T3SS, maintains invasion of host cells more efficient. Thus, inhibitory aptamers against SipA protein were developed using magnetic bead-based Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. These results represent a corner stone for future studies that could aim to develop putative inhibitors against Salmonellosis."Not applicable10001330
ABdb_1525 33585756 ssDNAHPA-2Helicobacter pylori (H. pylori) 5′AAGGAGCAGCGTGGAGGTTACCAGGAGGACCCTATTCTCGTGTATCGACGAGATCCAGTGACCACGACGACACACCCTAA3′ 800.55Kd: 19.3 ± 3.2 nM19.35′-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3′40100 mM NaCl, 5 mM KCl, 50 mM Tris–HCl, and 1 mM MgCl2, pH 7.5MgClTris Buffers7.5Not reportedDetection: " A new competitive detection method for H. pylori based on HPA-2 was then developed and optimized to detect H. pylori and showed no specificity for other bacteria. Moreover, we developed a new sensor to detect H. pylori with the naked eye for 5 min using illumination from a hand-held flashlight. In food inspection, this sensor could distinguish positive and negative using the naked eye within 5 min. Our results indicated that this sensor could be used to screen food samples. Our study provides a framework for the development of other aptamer-based methods for the rapid detection of pathogenic bacteria."Not applicable100013727164
ABdb_0781 28121169 ssDNA12L12AGroup A streptococcus (GAS) serotype M3 5'GCCTGTTGTGAGCCTCCTAACGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATACATGCTTATTCTTGTCTCC3' 780.5Kd: 8.25 ± 1.43 pM0.008255′-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3′38Phosphate-buffered saline (PBS) and 0.05% Tween 20, 1% bovine serum albuminNonePBS/phosphate buffers7.4Not reportedBiosensor: " Group A streptococcus (GAS) is an important Gram-positive pathogen that causes various human diseases ranging from peripheral lesions to invasive infections. The M protein is one of the main virulence factors present on the cell surface and is associated with invasive GAS infections. In this study DNA aptamers with a high binding affinity towards S. pyogenes serotype M3 were selected through 12 iterative rounds of the Systematic Evolution of Ligands by EXponential (SELEX) enrichment. The isolated aptamer can be used in any tool, such as a biosensor, for the detection of S. pyogenes and can be used in the development of a novel M-typing system."Not applicable10001139
ABdb_0780 28121169 ssDNA12L18AGroup A streptococcus (GAS) serotype M4 5'GCCTGTTGTGAGCCTCCTAACTCCTCGAGGGGGGGGGGATGAAAAGGAAAACGCAACAACATGCTTATTCTTGTCTCC3' 780.5256410256Kd: 7.47 ± 1.72 pM0.007475′-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3′38Phosphate-buffered saline (PBS) and 0.05% Tween 20, 1% bovine serum albuminNonePBS/phosphate buffers7.4Not reportedBiosensor: " Group A streptococcus (GAS) is an important Gram-positive pathogen that causes various human diseases ranging from peripheral lesions to invasive infections. The M protein is one of the main virulence factors present on the cell surface and is associated with invasive GAS infections. In this study DNA aptamers with a high binding affinity towards S. pyogenes serotype M3 were selected through 12 iterative rounds of the Systematic Evolution of Ligands by EXponential (SELEX) enrichment. The isolated aptamer can be used in any tool, such as a biosensor, for the detection of S. pyogenes and can be used in the development of a novel M-typing system."Not applicable10001140
ABdb_0843 28818557 ssDNAAM-6Escherichia coli (E. coli) O157:H7 5'CGTGATGATGTTGAGTTGGGGTGATGGGTGCATGTGATGAAAGGGGTTCGTGCTATGCTGTTTTGTCTAATAATACTAGTCCTTGCCAAGGTTTATTCCAGTAATGCCAACCAATCT3' 1170.4358974359Kd: 107.6 ± 67.8 pM107.65'-CGTGATGATGTTGAGTTG-N80-CAGTAATGCCAACCAATCT-3'80100 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl, 5 mM KClMgClTris Buffers7.4Not reportedDiagnostic: " In this study, systematic evolution of ligands by exponential enrichment using whole cells (Cell-SELEX) method was used for recognizing E. coli strain, O157 by single-stranded DNA library of aptamer. The isolated aptamer efficiency was confirmed and it was shown that the new DNA aptamer sequence has the ability to use for detection. This specific O157:H7 aptamer have the potential for application as a diagnostic ligand and could be used for detection of the related food borne diseases."Not applicable10001184
ABdb_1504 33379005 ssDNAXK-10Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) specifically on KPC-2 Escherichia coli (E. coli) 5′GACAGGCAGGACACCGTAACGGCAGGACACCGTAACGGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTGCTGCTCTGCTACCTCCCTCCTCTTC3′ 1010.603960396Kd: 0.81 ± 0.13 nM via SPR & 12.9 ± 1.03 nM via microarray chip 0.815′-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3′ also 5′-GACAGGCAGGACACCGTAAC-3N-GTCC-5N-GGAC-2N-GCC-4N-GGC-3N-GTTACGGTGCTGCTACCTCCCTCCTCTTC-3′401 × PBSMCT: 137 mM NaCl, 2.7 mM KCl, 5 mM MgCl2 and 1 mM CaCl2, 0.05% Tween-20, pH 7.4MgCl/CaClPBS/phosphate buffers7.4Not reportedDiagnostic: " Aptamer XK-10 was specifically bound to KPC-2 that identified by fluorescence polarization, and was also used to detect KPC-2. In addition, two methods were used to verify the specificity of aptamer XK-10 to KPC-2 E. coli and further suggesting that XK-10 could specifically recognize KPC-2 protein on the surface of KPC-2 E. coli. The Precision-SELEX was more efficient than traditional bacteria screening and could also be extended to screen other bacteria that express other kind of enzymes. recision-SELEX could simplify screening process and provide an accurate and efficient method to select aptamers for bacteria."Not applicableutilized two pools, however XK-10 sequence was most present in both end round libraries | utilized Precision-SELEX. . Primers for PCR amplification consisted of TAMRA-modified forward primer (TAMRA-FP) and biotinylated-modified reverse primer (Biotin-RP). TAMRA-FP: 5′-TAMRA-GACAGGCAGGACACCGTAAC-3′, Biotin-RP: 5′-Biotin-GAAGAGGAGGGAGGTAGCAG-3′.10001346