AptBacterialDB

Browse by Affinity Determination Method

This page provides a statistical overview of aptamer entries categorized by the experimental methods used to determine binding affinity. For more information refer to the User Guide.

Affinity Determination Method Statistics (Total Entries: 2131)
Affinity Determination Method (45) Count
N/A 591
Flow Cytometry 406
Fluorescence Spectroscopy 182
Surface Plasmon Resonance (SPR) 141
Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) 138
Radiolabel Equilibration Binding Assay 86
Fluorescence Binding Assay 63
Aptamer-Linked Immobilized Sorbent Assay (ALISA) 58
Enzyme-Linked Immunosorbent Assay (ELISA) 40
Modified Western Blot Assay 40
Isothermal Titration Calorimetry (ITC) 34
Enzyme-Linked Oligonucleotide Assay (ELONA) 31
Bioluminescence-based Membrane Blotting Assay 28
Bio-Layer Interferometry (BLI) 26
Colorimetric Peroxidase-Based Aptamer Plate Binding Assay 25
Nitrocellulose Filter Binding Assay 23
Surface Plasmon Resonance (SPR) and Microarray Chip 21
Radiolabel Filter Binding Assay 21
Saturation Binding Assay 19
Equilibrium Binding Assay 17
Electrophoretic Mobility Shift Assay (EMSA) 16
Indirect Enzyme-Linked Aptamer Assay (i-ELAA) 14
Bead-based Binding Assay 13
Micro-Fluorospectrophotometer (Fluorescence Spectroscopy) 13
Fluorescence Microscopy 13
Quartz Crystal Microbalance (QCM) 11
Fluorescence Microplate Reader (Fluorescence Spectroscopy) 10
Bead-based Affinity Chromatography 8
Fluorescence anisotropy (FA) 6
Ribogreen dye-based fluorescence intensity assay 5
Indirect ELASA (aptamer linked immunosorbent assay) 5
Enzyme-Linked Aptamer Assay (ELAA) 4
Fluorescence Resonance Energy Transfer Assay (FRET) 4
Nitrocellulose Filter Retention Assay 3
Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA) 3
Radiolabeled Binding assay 3
Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) 2
Metallo-β-lactamase activity assays 1
LPS-Aptamer Plate Binding Assay 1
Electrochemical impedance spectroscopy (EIS) 1
Binding Assay 1
Linearized adsorption isotherm 1
Surface Plasmon Resonance (SPR) and Enzyme-Linked Apta-Sorbent Assay (ELASA) 1
ECL-based and Colorimetric Peroxidase-Based Aptamer Plate Binding Assay 1
Enzyme-Linked Immunosorbent Assay (ELISA)-like Aptamer Microplate Affinity Ranking (ELASA) 1

Affinity Determination Method Distribution