AptBacterialDB

Year : 2015

Total records found: 229

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0502 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 3GGGAGCTCAGAATAAACGCTCAA-GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.13.8% survival ratio of living cells in biofilms with 1.1 μM aptamer and 5 μg/mL ampicillin sodium.14Fluorescence Spectroscopy41 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0503 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 1GGGAGCTCAGAATAAACGCTCAA-GGCCAGCAGCGAGTGTGGAGTATTGTGTTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy50 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0504 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 2GGGAGCTCAGAATAAACGCTCAA-GGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy54 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0505 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 4GGGAGCTCAGAATAAACGCTCAA-GGCACGGCATGTGTGGTATGTGGTGCCTGTACTCG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy53 ± 5 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0506 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 36CCGTCAGCCGAGGACCACAC-TTGGTTGCTGAATCCCCTCGTCTTGGCTTTCTTTGTCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.Mtb36 aptamer bound to M. tuberculosis H37Ra bacteria more than 4 times for M. bovis and 70 times for E. coli bacteria.7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)5.09 ±1.43 nMDetectionWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0507 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 17CCGTCAGCCGAGGACCACAC-GCGTCAATATGCTCGAGTCCCTTACTCCGTAATCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0508 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 57CCGTCAGCCGAGGACCACAC-GAGGCGGGGCGTACATGATGGAGGTGTTGTGTTCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0509 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 25CCGTCAGCCGAGGACCACAC-ATAGGAAGTATGCGGTCGTAGTTAATCGCCTGTCCTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0510 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 32CCGTCAGCCGAGGACCACAC-CTGGGCTCACTCACTGGCGTATCATTCGTCCGCGGTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0511 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 3CCGTCAGCCGAGGACCACAC-CGCTTCACGTGTCAGTGAATTTTCTCCATCGTTTGGTGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0512 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 40CCGTCAGCCGAGGACCACAC-TGTAGCGCATCTACGGGCTCTTCATTACGTCTATATCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0513 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 33CCGTCAGCCGAGGACCACAC-TTCACACACGGGTATAAACGCTCTGGTATGTCAAGCCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0514 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 35CCGTCAGCCGAGGACCACAC-TCGGACCTAGTGCTAGGGTCTCTAAGAAGTATCGGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0515 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 49CCGTCAGCCGAGGACCACAC-TTCTCCGATCTTAGTCAACTTGGACCATGAATGCGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0516 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 46CCGTCAGCCGAGGACCACAC-TATTTACGCTGACCGACGATTTTCTATCAGAGTGCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0517 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 13CCGTCAGCCGAGGACCACAC-CGATCACTGTACAGTCCTGGATAAGCCGTTCTTTCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0518 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 11CCGTCAGCCGAGGACCACAC-CGCCGGCCTTCTTACAAGACCTGTTCAATTCCCAGTGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0519 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 50CCGTCAGCCGAGGACCACAC-AGGCTCACGCCCTGTCAATACTGCCTCTTGTCCTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0520 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 38CCGTCAGCCGAGGACCACAC-ATTCGGCTGAAGACCCGAGCCGGTCATCCGGTGTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0521 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 28CCGTCAGCCGAGGACCACAC-CTCCATCACGTGTAGTCAGCTGACCATTGATCGTGCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0522 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 8CCGTCAGCCGAGGACCACAC-CTATCCCGTGGTCCATTGCTTTTCCCGGTCTCTTCTCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0523 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 52CCGTCAGCCGAGGACCACAC-CTTTGTGCCGGGAAAAGACTGGCCTGTGTTGACTTGCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0524 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 2CCGTCAGCCGAGGACCACAC-CGGCGCTTTTCTCATCCTACCTCCGCTCTTACCTGTATGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0525 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 9CCGTCAGCCGAGGACCACAC-GCCGTGCTGAGTCTGTCAGCAGTTTGCTAGTCTTCCCTGC-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0526 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 44CCGTCAGCCGAGGACCACAC-TTACACGAAGGCTCGGCCCATCTCTCTCGTGTCACTTCGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0527 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt1ATGCGGATCCCGCGC-CGAGTGAGGGCGAGGCGCGCTCCTGCCGGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.IC₅₀ of 28.94 ± 0.002 nM and showed a MIC of 5.36 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.10Enzyme-Linked Immunosorbent Assay (ELISA)1.06 ± 0.10 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0528 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt6ATGCGGATCCCGCGC-CGGCCAGGGGACGAGCGCGCCCTGATCGTG-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.IC₅₀of 22.35 ± 0.001 nM and showed MIC of 6.24 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.10Enzyme-Linked Immunosorbent Assay (ELISA)0.210 ± 0.05 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0529 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseApt1_M3 (17-mer)ATGCGGATCCCGCGC-GAGGGCGAGGCGCGCTC-GCGCAAGCTTCGCGC475'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 5.36 μg/ml.10Enzyme-Linked Immunosorbent Assay (ELISA)4.90 μMTherapeuticsDNA-SELEX5'-BiotinylatedMtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.N/AN/AN/AN/A
ABdb_0530 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseApt6_M3 (20-mer)ATGCGGATCCCGCGC-CAGGGGACGAGCGCGCCCTG-GCGCAAGCTTCGCGC505'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 6.24 μg/ml.10Enzyme-Linked Immunosorbent Assay (ELISA)364 nMTherapeuticsDNA-SELEX5'-BiotinylatedMtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.N/ABest CandidateN/AN/A
ABdb_0531 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt2ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)0.286 ± 0.64 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0532 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt3ATGCGGATCCCGCGC-GCCCACCTGTGGGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Showed moderate to lower inhibition specificities.10Enzyme-Linked Immunosorbent Assay (ELISA)0.677 ± 0.14 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0533 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt4ATGCGGATCCCGCGC-GCCCACGTGTGGTGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Showed moderate to lower inhibition specificities.10Enzyme-Linked Immunosorbent Assay (ELISA)0.956 ± 0.20 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0534 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt5ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)2.03 ± 0.12 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0535 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt7ATGCGGATCCCGCGC-ACGCGACAGCAGTGCGCGCCCCGTCCCGGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)1.02 ± 0.13 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0536 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt8ATGCGGATCCCGCGC-CGACGGAGGGAGGCGCGCCACACTGGGTGC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)0.55 ± 0.05 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0537 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt9ATGCGGATCCCGCGC-GCACCGGCAGGAGCGCGCCTCGCCCTCACT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)1.66 ± 0.22 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0538 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA11ATACCAGCTTATTCAATT-TAGGCGGTGGAGATAGTAAGTGCAATCTAGGCGGTGGAGATAGTAAGTGCAATCTATGCC-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.Inhibited SEB-mediated PBMC proliferation of 93% and TNF-α, IL-1β, IL-6, IL-2, and IFN-γ in culture supernatants were reduced by 77, 97, 64, 96, and 99%, respectively. 90% survival of mouse models of SEB-induced TSS.11Fluorescence Spectroscopy64 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FITC LabeledShowed no cytotoxic effects on PBMCs.N/AN/AN/AN/A
ABdb_0539 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA2ATACCAGCTTATTCAATT-GATCGATCGATCAGTCAGTCGACCAACGCACGACACGCATCGCTCTATCTCGTCGAACAG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.A2 achieved 34% PBMC proliferation inhibition.11Fluorescence Spectroscopy26 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0540 258914722015Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogenSA20hpGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-gated nanocapsules for the specific targeting of vancomycin to bacteria for the controlled release of vancomycin.15-fold increase of efficacy for vancomycin with MIC of 0.420 μg/mL.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-AlexaFluor 488 Labeled and 3'-(PEG)-AGGGCGC-BH1MICs of vancomycin-nanoparticles for S. epidermidis (control) were found to be 6.295 µg/mL.N/AN/AN/AN/A
ABdb_0541 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.Have a limit of detection of 6 ng/mL with a range of 0.01–10 μg/mL of SEC1.12Fluorescence Spectroscopy65.14 ± 11.64 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0542 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C2C36.2AGCAGCACAGAGGTCAGATG-TATCAGAATTAATGCTCTCGTAATTTTCGAATCGTCGTGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy49.43 ± 11.76 nMBiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0543 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C3C34.1AGCAGCACAGAGGTCAGATG-CTTTCATTTTTATTCTTTTTGACTGTATTTTATGTACCAT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0544 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C4C4AGCAGCACAGAGGTCAGATG-CTAACCTAATTCGACCGTGTATTCTTCTGCGTTATTTACC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0545 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C5C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0546 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C6C9AGCAGCACAGAGGTCAGATG-TCCATTATCAGGTTCTTTATTCTGTTGTTCAACTTATTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy154.9 ± 45.67 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0547 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C7C32.1AGCAGCACAGAGGTCAGATG-TTTTAGATTTAGCTCTTATTTGTTCGAGCAATCCCAAAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0548 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C8C2AGCAGCACAGAGGTCAGATG-GCCAACTCAATTTTTTGTTTTGTCTTTCCGATTGATCTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0549 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C9C6AGCAGCACAGAGGTCAGATG-CATATCGAATTTTCCTCGTGATTTTTCTTTCATTCTTAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0550 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C10C11AGCAGCACAGAGGTCAGATG-TAGTTAATGGATTTTTCTTCTTTATCTTCTTATTTCTTGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0551 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C11C30AGCAGCACAGAGGTCAGATG-ATGTTTTCCCTTATCACTACTTTTATTTGTCTTTTTGCAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0552 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12UGUAAUUCUGCCAUUCUUUUUGGGGCGGAAUACAGGAUGU405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.fmA12-functionalized AgNPs resulted in specific S. aureus antimicrobial activity with only a 1.6 ± 1.0% survival of 12598 cells and 51.9 ± 2.0% survival of 10832 cells.8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)95 nM (by SPR) and 67.1 ± 6.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/A76% fmA12 survives in 10% mouse serum at 37°C over 24 h, and in 50% serum, 85.0% fmA12 survives after 5 h.N/A~52 h for fmA12.N/A
ABdb_0553 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmF07AUACGCUGCUCACUAAAGUCGGGAGUACUCCAAUGUUUUC405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)38.9 ± 4.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_0554 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmE09AUCUAGCAAGCACUUGCAAACAUUCGAUAACGGGGAUUC395'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)418 ± 112 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0555 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmG12UGCCAAUGUAACUCAACACUCUUCAUUGAGCUGUCGGGCA405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)475 nM (by SPR) and 220 ± 24 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0556 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ3N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.7 ± 3.5 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0557 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ6N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)63.1 ± 7.3 μMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0558 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ9N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.1 ± 16.7 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0559 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry7 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0560 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20PTTCACGGTAGCACGCATAGG-CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 61% increase in the gated fluorescence intensities above background.8Flow Cytometry12 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0561 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry71 ± 23 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0562 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.D-Cells 9P showed an increase in the gated fluorescence above background by 65%.8Flow Cytometry44 ± 8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0563 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT79N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow Cytometry20 ± 3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0564 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG39N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0565 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 20PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAATAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0566 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGAGGCAATGCCTTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1075'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0567 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA14CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT-GGTGGATCCATATTCCTACTCG1085'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence Spectroscopy3.49 ± 1.43 nMDetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0568 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0569 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA20CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCAGCGGGGGCTGCGCGGTGGAGTGCTGTGGGCG-GGTGGATCCATATTCCTACTCG985'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0570 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGTG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0571 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGTCGGTGTCTGCCGGGGGATGTGGAGGCTGGGTGTTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0572 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGCGGGCTACCTGGCTAGTACGCCATGATGCCTGCACGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0573 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0574 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.PA#2/8 competes with IgG or IgG-Fc for binding to Protein A.11Bead-based Binding Assay1.06 ±0.2 μMDetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0575 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/22ATACCAGCTTATTCAATT-GCAGTACTGATGAGTGTAGCCGTATGATTATCGTTTGTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0576 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/34ATACCAGCTTATTCAATT-CCCCAACGAGTCGATATGTAGCCCACACTCTGATTCGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0577 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/11ATACCAGCTTATTCAATT-GGAGACGACAAACTATTACGTACTACGGCATGCACTTGGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0578 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/3ATACCAGCTTATTCAATT-CGACAAGTGGGCATTACGATTCTAGCCCTGATTATGTTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0579 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/6ATACCAGCTTATTCAATT-ACCGATCACTAGCCGACTAATTGGTTTCCGATCGCAGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0580 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#14/82ATACCAGCTTATTCAATT-CCACAACCGAACTCGTAAGACGTATGTAGCCGCCAACTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0581 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/52ATACCAGCTTATTCAATT-ACGCATTGGAGCCCGAAACTGATTCATTGAGCCTACCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0582 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/14ATACCAGCTTATTCAATT-ACGACCGTAGACGACTTACACTGATGTTGCGCATTTCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0583 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/31ATACCAGCTTATTCAATT-CGATGACGACTGTAGCCGCAATACGCCCCTGTTACGTTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0584 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/41ATACCAGCTTATTCAATT-GGACGCCGACTAACTTTACGTGGTTCTCCTACCGCCTAACC-ACAATCGTAATCAGTTAG775'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0585 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/43ATACCAGCTTATTCAATT-ACGAAATGTAGCCGATCCTGATTACTCTCTGTCAGCTTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0586 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-1CGGATCCATGGGCACTATTTATATCAA-TATGCCAGCATTTCGCCAACGGTGGTCATACAGTGTGAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0587 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-2CGGATCCATGGGCACTATTTATATCAA-GGGTATCCCACTCGGACGTTCATACCTGGCTGGTTGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0588 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-3CGGATCCATGGGCACTATTTATATCAA-AGCGCGTTTTGGACATGCGCTAGCTTCAATTTGAGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0589 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-4CGGATCCATGGGCACTATTTATATCAA-GCCAATGCACTCTGGTTATTCCCCTAAACATCCCGGAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0590 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-5CGGATCCATGGGCACTATTTATATCAA-CGTTTGGGGCTGTGTTGCAAGACCGTACGTTGCCCCCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0591 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-6CGGATCCATGGGCACTATTTATATCAA-TGCAACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0592 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-7CGGATCCATGGGCACTATTTATATCAA-TGGTACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0593 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-8CGGATCCATGGGCACTATTTATATCAA-TTACAGTATCCTCCCACGTTGGTTCGTGTCTTACGGAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0594 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-1CGGATCCATGGGCACTATTTATATCAA-CCCGTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0595 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-2CGGATCCATGGGCACTATTTATATCAA-GGCTAGGCCCCGGATCACCACTTTCCCTGCTTGATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0596 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-3CGGATCCATGGGCACTATTTATATCAA-CCTTACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0597 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-4CGGATCCATGGGCACTATTTATATCAA-GGCGTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0598 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-5CGGATCCATGGGCACTATTTATATCAA-CTGCACGTGGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0599 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-6CGGATCCATGGGCACTATTTATATCAA-GACTACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0600 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-7CGGATCCATGGGCACTATTTATATCAA-GCCATTCGTCACTGAGTTGCTCTAGTGCCGTGTGCTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0601 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-8CGGATCCATGGGCACTATTTATATCAA-CTAAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0602 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-1CGGATCCATGGGCACTATTTATATCAA-GCATCGGTCCTGAAGTTGCTCTAGTGCCCGTGTGCTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0603 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-2CGGATCCATGGGCACTATTTATATCAA-GGGCTAGCCCCGGATCACCACTTTCCCTGCTTGATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0604 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-3CGGATCCATGGGCACTATTTATATCAA-TGTAACGGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0605 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-4CGGATCCATGGGCACTATTTATATCAA-TGGCACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0606 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-5CGGATCCATGGGCACTATTTATATCAA-CCTAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0607 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-6CGGATCCATGGGCACTATTTATATCAA-CGGTTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0608 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-7CGGATCCATGGGCACTATTTATATCAA-GTGTTTCGCGCTATTTCCGTACAACCCGCGGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0609 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-1CGGATCCATGGGCACTATTTATATCAA-CCTCACATAGTGACGATGTGGTTTGGTACCTCTATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0610 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-2CGGATCCATGGGCACTATTTATATCAA-CCGTGGATGTACGAAATTAACCGCACACCTAGCTACCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0611 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-3CGGATCCATGGGCACTATTTATATCAA-CGCATGCTTCTCGGGATACGGGCGGCTCGATAGAGTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0612 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-4CGGATCCATGGGCACTATTTATATCAA-GCTTACTACGTTTGCCTCAGTGTGTTCCGGTCTTAACAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0613 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-5CGGATCCATGGGCACTATTTATATCAA-CCCATACCTTTTTCGATAGTAAGTGCCATGCCCATCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0614 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-6CGGATCCATGGGCACTATTTATATCAA-GCCTAGAACCCCCTGTCTAGTCAAGTAGTGCGAGTGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0615 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-7CGGATCCATGGGCACTATTTATATCAA-GCCTTACCGCTCTATCACTCGTCTGTTGCCACTCAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0616 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-8CGGATCCATGGGCACTATTTATATCAA-TGCGCCGATAGTTTCGATCATGATGCATCTGGCTGTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0617 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-9CGGATCCATGGGCACTATTTATATCAA-CGGTAGTACTACTGGAGACAACTTCGCATACTTTAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0618 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-1CGGATCCATGGGCACTATTTATATCAA-TTGCTACAGGTATTGCACAACCTCGTGTGGTGTCCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0619 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-2CGGATCCATGGGCACTATTTATATCAA-CGGTAGTACTACTGGAGACAACTTCGCATACTTTAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0620 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-3CGGATCCATGGGCACTATTTATATCAA-GCTGGCAACTGGATGCACTCGTCCTCAGCCTCGGTCAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0621 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-4CGGATCCATGGGCACTATTTATATCAA-GCCCGTGAGACTATACCCTATGCACTAGTTGCGTAATAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0622 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-5CGGATCCATGGGCACTATTTATATCAA-GCTCGCGTCTGAGGGAAGTTACGTTATTTCGCTTGTAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0623 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-6CGGATCCATGGGCACTATTTATATCAA-CCGGACTTTAAGCGGCTTCTCGTGGCTGGGTAATCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0624 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-7CGGATCCATGGGCACTATTTATATCAA-CCTGACCGATGTTGTATTAATGGCTCCGGGTCTTATGAG-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0625 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-8CGGATCCATGGGCACTATTTATATCAA-CGAGGGGTTGGTTTGTGGTGTTGCCGCCACTACAGCCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0626 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusPrimary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A35 nMBiosensorWhole Cell-SELEX5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0627 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusSecondary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A129 nMBiosensorWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0628 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA1UUCAGGGACCAGUGUUUGCCUUGUGCCAGUC315'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay108 ± 33.4 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0629 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA2CCCAUAGUGAGUCGUAUUAAUUG235'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay296 ± 57.1 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0630 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA3GCGACCCUAUAGUGAGUCGUAUUAAUGCGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay388 ± 79.2 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0631 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA4GCGCCCCUAUAUGAGUCGUAUUAAUUGCGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay105 ± 10.9 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0632 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA5GCGCCCCCAUAGUGAGUCGUAUUAAUACGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.ABA 5 rescued cells to at least 50% maximum cell viability, with an IC50 value of 5 μM.8Ribogreen dye-based fluorescence intensity assay205 ± 69.8 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0633 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA1GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)12.02 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0634 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA2GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0635 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE6-15GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0636 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-3GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0637 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-4GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0638 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE7-12GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAGTAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0639 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-5GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGTCTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0640 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE9-16GGGAGCTCAGAATAAACGCTCAA-CGGGCTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0641 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP6-5GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGCTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0642 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-4GGGAGCTCAGAATAAACGCTCAA-TACCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0643 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-8GGGAGCTCAGAATAAACGCTCAA-CATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0644 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCCTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.Display a sensitive detection of 200 nM alpha toxin.12Surface Plasmon Resonance (SPR)93.7 ± 7.0 nMDetectionSELEX5'-Amidation (NH₂-(CH2)6) or 5'-FAM LabeledN/AN/AN/AAverage half-life of ssDNA MREs in serum is about one hour.N/A
ABdb_0645 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.26TGTACCGTCTGAGCGATTCGTAC-CCTTGCCGATGCCTTTACGGTCTAGTTTGGATGT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0646 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.01TGTACCGTCTGAGCGATTCGTAC-TCGGGCGATGATACTTAGCACGGTCTAGGTCAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0647 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.20TGTACCGTCTGAGCGATTCGTAC-TAGCGGCAGAGTAGCACTCTATAGGTCGATGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0648 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.02TGTACCGTCTGAGCGATTCGTAC-CGTGTCCTATTTTCTTCTCTGTTAACTCTCGTC-AGCCAGTCAGTGTTAAGGAGTGC795'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0649 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCTTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0650 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.39TGTACCGTCTGAGCGATTCGTAC-TTTGATCTCGTGTGTCTAGTTGCGGCGGATTGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0651 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.10TGTACCGTCTGAGCGATTCGTAC-GGTCAACCTCACCGACTGCCGACCGTTTAATTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0652 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.43TGTACCGTCTGAGCGATTCGTAC-CGTCATTGCCTCGTAGTATTCTTATAGTCGGTAG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0653 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.44TGTACCGTCTGAGCGATTCGTAC-TCCCGAAAGCGCGTCAGCCTGGGAGGTTATGCGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0654 255909732015Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenesLMCA2ATACCAGCTTATTCAATT-CCAAAAGCGCACCCATATATGTTCTATGTCCCCCACCTCG-AGATTGCACTTACTATCT765'-ATACCAGCTTATTCAATT-N40-AGATTGCACTTACTATCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinDeveloped an aptamer-based sandwich assay (ABSA) platform to distinguish L. monocytogenes from other Listeria species and other bacterial genera.Exhibited a linear response from 20 to 2×106 CFU per mL with a LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)2.01×10(-12) MBiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0655 255909732015Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenesLMCA26ATACCAGCTTATTCAATT-CGATGGGGATTACTTATCATGAGAAACCAGCAGCATTTG-AGATTGCACTTACTATCT755'-ATACCAGCTTATTCAATT-N40-AGATTGCACTTACTATCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinDeveloped an aptamer-based sandwich assay (ABSA) platform to distinguish L. monocytogenes from other Listeria species and other bacterial genera.Exhibited a linear response from 20 to 2×106 CFU per mL with a LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)1.56×10(-10) MBiosensorWhole Cell-SELEXCyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_0656 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.33TGTACCGTCTGAGCGATTCGTAC-CATAGGGTGCTTTTCAAGGCCACACGTTAGTGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.100 nM or 6.6 μg/mL of Exotoxin A in human serum was detected.14Surface Plasmon Resonance (SPR)4.2 and 4.5 μMDiagnosticDecoy-SELEX5'-Amidation (NH₂-(CH2)6) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0657 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.02TGTACCGTCTGAGCGATTCGTAC-TACGCCACACGTGGTGAGGGATTCGATCGCTTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0658 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.20TGTACCGTCTGAGCGATTCGTAC-TGCTATTCATCACCACTCTAGAGCCACTTTTAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0659 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.22TGTACCGTCTGAGCGATTCGTAC-TGGGCGGCGAGCCACCCGGCAATTTAGTACAGGC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0660 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.36TGTACCGTCTGAGCGATTCGTAC-AAAGCATCCAGCCGGTATGTGCCAGAGTCTCTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0661 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.38TGTACCGTCTGAGCGATTCGTAC-AAATGTGAGTGGCCAGGCATCAGGTACGTCGGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0662 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.03TGTACCGTCTGAGCGATTCGTAC-GGATAGGTGCCTCTGCTTCATCATGTTGAACTTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0663 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.13TGTACCGTCTGAGCGATTCGTAC-AGTTTCACCAGTCGCCTGTTAGCCGTGATATACG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0664 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.27TGTACCGTCTGAGCGATTCGTAC-TGTCAATATTACGTTGCTCTTAGGTTCACCATCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0665 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.28TGTACCGTCTGAGCGATTCGTAC-TTGTGATTCAAATAGGCGTGTTGGTGTGAGACCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0666 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.23TGTACCGTCTGAGCGATTCGTAC-CGTGGATCATGCTTCGCGTCGGTTTATAGGTTCC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0667 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.39TGTACCGTCTGAGCGATTCGTAC-AGAAGAGCATCGGTAACTTCCATAGGAGATGGGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0668 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.06TGTACCGTCTGAGCGATTCGTAC-CATCCGAGGGTATTGTATGCGTATATCCTAGTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0669 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.10TGTACCGTCTGAGCGATTCGTAC-CAAGTTCCTCATGGAGGGTGCTCAGAGCTTAGAC-AGCCAGTCAGTGTTAAGGAGTAA805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0670 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.34TGTACCGTCTGAGCGATTCGTAC-AGGGGGATTCCTAGGGCCCGGCCCAACGCTGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0671 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.42TGTACCGTCTGAGCGATTCGTAC-CAAGACCCTTGAATCACGGGTAGGGTCTCGTAAC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0672 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesLac-aptAGCAGCACAGAGGTCAGATGTAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTGCCTATGCGTGCTACCGTGAA78N/AssDNALactobacillus Acidophilus (KCTC 3164)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/A13 ± 3 nMBiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0673 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesSty-aptTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 2421)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0674 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesPae-aptCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 15692)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/A17.27 ± 5 nMBiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0675 https://doi.org/10.1007/s00604-015-1649-72015Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubesAnti-Salmonella aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDevelop a label-free impedimetric aptasensor based on an amino-modified aptamer bound to rGO-MWCNT composite for the detection of Salmonella.Exhibits a linear range from 75 to 7.5 × 10(5) cfu/mL and a detection limit of 25 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0676 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P (Parental)AGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal, 3′-FAM α20A24P significantly increased phagocytosis in the presence of either transgenic mouse IgG or hIVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled, 5′-α-Gal or Biotin-TEG modifiedN/AN/AN/AN/AN/A
ABdb_0677 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A2AGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCT69N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0678 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A3AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCC52N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal α20A24P.A3 significantly increased phagocytosis in the presence of either transgenic mouse IgG or human IVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled and 5′-α-GalN/AN/AN/AN/AN/A
ABdb_0679 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A4AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCA53N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0680 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A5AGGTCAGATGGGGGGAAGACAC22N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0681 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A6AAGGCCGGGGTGAAGTGTAGAGGCCTA27N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0682 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A8TGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGC43N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0683 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A9AGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTAT57N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0684 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer15A3PTTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGGCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0685 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A1PTTCACGGTAGCACGCATAGGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0686 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8PAGCAGCACAGAGGTCAGATGCCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0687 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0688 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9PAGCAGCACAGAGGTCAGATGCACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGGCCTATGCGTGCTACCGTGAA79N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0689 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG39N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0690 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A12PTTCACGGTAGCACGCATAGGGCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCCCATCTGACCTCTGTGCTGCT78N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0691 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A14PAGCAGCACAGAGGTCAGATGGGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0692 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0693 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 2TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0694 257022752015A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplificationAmplification-aptamer (a-aptamer)ATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGT49N/AssDNAEscherichia Coli (E. Coli) O157:H7Outer membrane protein (OMP)Developed a Lateral Flow Biosensor (LFB) with gold nanoparticle (AuNP) probes for the detection of E.coli.Can detect as low as 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0695 257022752015A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplificationCapture-aptamer (c-aptamer)TTTCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG45N/AssDNAEscherichia Coli (E. Coli) O157:H7Outer membrane protein (OMP)Developed a Lateral Flow Biosensor (LFB) with gold nanoparticle (AuNP) probes for the detection of E.coli.Can detect as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0696 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 70.86 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0697 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 61.50 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0698 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 72.42 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0699 254611752015A new aptamer/graphene interdigitated gold electrode piezoelectric sensor for rapid and specific detection of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped an aptamer/graphene interdigitated gold electrode piezoelectric sensor to detect Staphylococcus aureus.Achieved a detection limit of 41 cfu/mL with a linear range of 4.1×10(1) to 4.1×10(5) cfu/mL. The detection is rapid, completing in less than 60 minutes.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0700 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 15 cfu/mL for S. typhimurium.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0701 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 35 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0702 254675002015Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized core-shell type magnetic separationAptamerCTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-based QCM biosensor for the determination of Salmonella in food samples.Showed specific detection of Salmonella cells at 100 CFU/mL from a milk sample and a linear response range from 100 to 4 × 10(4) CFU/mL cells.N/AQuartz Crystal Microbalance (QCM)3259 CFU mL−1BiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0703 https://doi.org/10.1080/00032719.2015.10362782015Aptamer Immobilized Magnetoelastic Sensor for the Determination of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop an aptamer-based magnetoelastic sensor for the detection of S. aureus.Linear dynamic range of 10^1–10^11 cfu/mL and detection limit of 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0704 https://doi.org/10.1039/C4AY02880E2015Rapid and sensitive detection of Salmonella typhimurium using aptamer-conjugated carbon dots as fluorescence probeS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a fluorescence probe based on amino-modified aptamer conjugated to carboxyl-modified carbon dots (CDs) for detection of Salmonella typhimurium.Limit of Detection (LOD): 50 cfu mL⁻¹ and linear range of 10(3)-10(5) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0705 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157EcO 4 RevACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA37N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0706 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157EcO 3 RevGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG36N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.Exhibited a linear range of 10-10(4) cfu/mL and the detection limit of 10 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0707 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157E17F-37ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT37N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_0708 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157E18R-42CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0709 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #7TCAGTCGCTTCGCCGTCTCCTTC-GGGGGCGCGGTGAGGGGCTGCACAAGAGGGAG-GCACAAGAGGGAGACCCCAGAGGG79N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #7 for inactivated V. alginolyticus was 10 cells/ml.N/AFluorescence Spectroscopy28.39 ± 11.46 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0710 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #8TCAGTCGCTTCGCCGTCTCCTTC-AGCCGGGGTGGTCAGTAGGAGCAGCACAAGAGGGA-GCACAAGAGGGAGACCCCAGAGGG82N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #8 for inactivated V. alginolyticus was 10 cells/ml.N/AFluorescence Spectroscopy12.82 ± 6.00 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0711 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #23TCAGTCGCTTCGCCGTCTCCTTC-GTAGGAGGTAGTCGGAGAGGCGAATGAGAGGGGAAGCACAAGAGGGA-GCACAAGAGGGAGACCCCAGAGGG94N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #23 for inactivated V. alginolyticus was 10(3) cells/ml.N/AFluorescence Spectroscopy46.89 ± 15.87 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0712 https://doi.org/10.1080/00032719.2015.10529742015Determination of Shigella flexneri by a Novel Fluorescent AptasensorAptamer1CCTCATGTCGAACAGCAACACTGCAACACTGTATAGTCCTGTGTGCCTTGAGCGTTTATTCTGAGCT67N/AssDNAShigella Flexneri (CGMCC 1.1868)Whole cellDeveloped a homogeneous fluorescent aptasensor using a dye-labelled aptamer and graphene oxide, using target recycling amplification for Shigella flexneri detection.A linear relationship was displayed from 500 to 10(9) CFU/mL with a limit of detection of 100 CFU/mL.N/AN/A29 ± 4 nMBiosensorN/ACarboxyfluorescein-GGGCCC at the 5' and 3' endsN/AN/AN/AN/AN/A
ABdb_0713 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp1TGAGCCCAAGCCCTGGTATG-TTCTTCCCTTTTATTAGTCCTGTATTCCTCTACTGTTGCC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry5.98 ± 0.835 nMDetectionWhole Cell-SELEX5'-FAM Labeled and BiotinylatedN/AN/AN/AN/AN/A
ABdb_0714 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp13TGAGCCCAAGCCCTGGTATG-TATCCGATTTTATCTGTCCAATATTCCCTGTGTCTACCTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry97.38 ± 36.2 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0715 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp16TGAGCCCAAGCCCTGGTATG-TATCTTCACTTGACCTTTACCGCAATCGTCCATGTATCTG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry682.2 ± 125 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0716 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp19TGAGCCCAAGCCCTGGTATG-TTCTACCCAACTGCTTTAATAGTCACTGTCATAGTTCCAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0717 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp20TGAGCCCAAGCCCTGGTATG-TCGTCGATTATTGTTTCAGTTCAGTTCCCCCGCGTTCCGA-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry14.32 ± 2.19 nMDetectionWhole Cell-SELEX5'-FAM Labeled and HEX (hexachlorofluorescein) LabeledN/AN/AN/AN/AN/A
ABdb_0718 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp22TGAGCCCAAGCCCTGGTATG-CCTACCTTAGTCCGTTTCGCTGTGTTTGTCCAATATTCCT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry4.98 ± 0.357 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0719 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp23TGAGCCCAAGCCCTGGTATG-CTTCACTTGACCCTTATCGCTTCTAACACAACCGCTTTTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry5.735 ± 0.478 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0720 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp28TGAGCCCAAGCCCTGGTATG-TATTCCCCCTTATTTTAGCCATTTTGTTACATCTTCCGTC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry72.89 ± 22.16 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0721 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp29TGAGCCCAAGCCCTGGTATG-ATATTCGTTCTTTTCGCCTTAACTTCTCGTGTTTCCTTAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry47.83 ± 9.7 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0722 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 10GATGATGGACGTATATCGTCTCCCATGAATTCAGTCGGACAGCG44N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0723 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 22ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGCG41N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0724 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 45ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGC40N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0725 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 60CGCCCACCCATAATGGATCAGGGCGGGCACCACGATG37N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0726 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 1CGAAGGGGCTATGCCGCCTACATAGACCGTCACGA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0727 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 2GGCCGGCAATACGGCCGAGCCCGGGGTTCCTCCGA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0728 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 3GCCACGCGCAGCAATCAAACCCGGCCCCCTGCTCC35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0729 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 4TGGCCAGAGTACGAGTAAGGGAGGTCACAACCTTA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_2016 255111122015Development of a fluorescent enzyme-linked DNA aptamer-magnetic bead sandwich assay and portable fluorometer for sensitive and rapid listeria detectionLLO-3N/AN/A5'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinA fluorescent DNA aptamer-magnetic bead sandwich assay was developed to detect LLO protein.Demonstrated LODs in the range of 4 to 61 L. monocytogenes cells or the equivalent LLO produced by 4 to 61 cells on average in a separate titration trial.8Enzyme-Linked Immunosorbent Assay (ELISA)-like Aptamer Microplate Affinity Ranking (ELASA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/APatent filled