Year : 2013

Total records found: 164

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0197 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (80nt)CTCCTCTGACTGTAACCACG-TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0198 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (60nt)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry7.8 ± 6.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0199 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6 (79nt)CTCCTCTGACTGTAACCACG-TAACGTACCAAAATGTTGGATTGGATGTTGTACTGGGTT-GCATAGGTAGTCCAGAAGCC795'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry53 ± 7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0200 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6_54TACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC545'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.3 ± 0.58 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0201 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_80CTCCTCTGACTGTAACCACG-CACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry28 ± 3.8 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0202 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_60CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis and 43 ± 4% (SE-20, 60nt) in S. enteritidis.12Flow Cytometry7.1 ± 0.62 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0203 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_80CTCCTCTGACTGTAACCACG-TATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry30 ± 4.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0204 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_60CTCCTCTGACTGTAACCACGTATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry5.3 ± 0.7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0205 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-11_60CTCCTCTGACTGTAACCACGAACGATTCAAGAACTGTTGGTTGTCGGCTTATTTTCGCCA605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.9 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0206 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-34_80CTCCTCTGACTGTAACCACG-TGCCGCTAAACGCCGGCTCATCGTTATGCTTTTCATTGCA-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry56 ± 7.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0207 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-1_40GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0208 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-6_60GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0209 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_80CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0210 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_60CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT595'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium and 62 ± 12% (ST-12, 60nt) in S. typhimurium.12Flow Cytometry4.5 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0211 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_80CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0212 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_40ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0213 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-33_60CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow Cytometry51.0 ± 4.3 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0214 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2PATAGGAGTCACGACGACCAGAA-AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.The limit of detection (LOD) was 25 cfu/mL, and the percentage gated fluorescence intensity above library background was 20% when ST9P was incubated with L. monocytogenes cells and 72% when incubated with S. typhimurium.9Flow Cytometry6.33 ± 0.58 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0215 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 82 ± 2.34% for ST2.9Flow Cytometry16.34 ± 0.45 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0216 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST3PATAGGAGTCACGACGACCAGAA-TTTCGGCTAAGGACGGGTGAAATACATTTAATAGGGTGGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0217 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST7PATAGGAGTCACGACGACCAGAA-TAAGTACAGGACTGGAGTATTAGCGGGGTCCATGCAAGGC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0218 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 72 ± 1.57% for ST9.9Flow Cytometry9.45 ± 0.63 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0219 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9PATAGGAGTCACGACGACCAGAA-AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 66 ± 1.45% for ST9P.9Flow Cytometry11.14 ± 0.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0220 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC4CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.C4 showed strong specific binding to S. Typhimurium, and the increase in the fluorescence intensity of C4 was less than 30% against E. coli and S. aureus.10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0221 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGTCGGAGCCAGGATGCGAGGTCTGTAGGTCTGCGGGCGG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0222 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGCGGGCGAGTTGACGGCGTAATCGTGCTGCCGCGTG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0223 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGGGCGTGGCTCAGAGTGGGGGTCGGTCAGTCTTGTGCGCG-GGTGGATCCATATTCCTACTCG1025'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0224 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC5CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGACGCCTGCGTGGCTGAGGCTTCGGTCTGCGCCG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0225 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGCGGGCAGGATGGGATGCTGTAGGTCTGCGGGCGCGCG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0226 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGCG-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0227 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC8CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGACGGGTACCGGGCGTGTGGCGTGCCTGCCGCG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0228 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC9CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGTGCGGGCCGGATGGGAGCTCTGTAGGTTGCGGGCGCGG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0229 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA1/SA-1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGA-GGTGGATCCATATTCCTACTCG825'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0230 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA2/SA-2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG835'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0231 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA3/SA-3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGAGGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0232 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt22GAATTCAGTCGGACAGCG-ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGCG-GATGGACGAATATCGTCTCCC805'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.LOD of the method was 10(3) cfu/mL with a range from 10(3) to 10(7) cfu/mL, and detection signals of target bacteria were 4.4-fold higher than S. Enteritidis, 4.3-fold higher than S. Arizonae, 56.3-fold higher than S. aureus, and 24.3-fold higher than E. coli K88.17Fluorescence Spectroscopy47 ± 3 nMBiosensorWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0233 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt10GAATTCAGTCGGACAGCG-GATGATGGACGTATATCGTCTCCCATGAATTCAGTCGGACAGCG-GATGGACGAATATCGTCTCCC835'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy73 ± 9 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0234 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt 45GAATTCAGTCGGACAGCG-ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGC-GATGGACGAATATCGTCTCCC795'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy68 ± 6 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0235 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt 60GAATTCAGTCGGACAGCG-CGCCCACCCATAATGGATCAGGGCGGGCACCACGATG-GATGGACGAATATCGTCTCCC765'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy56 ± 9 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0236 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA17TCCCTACGGCGCTAAC-CCCCCCAGTCCGTCCTCCCAGCCTCACACC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 312 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)35 nM and 3.03 nM for SA17-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0237 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA61TCCCTACGGCGCTAAC-CTCCCAACCGCTCCACCCTGCCTCCGCCTC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 1250 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)129 nM and 9.9 nM for SA61-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0238 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA1ATCCAGACGTGACGCAGC-ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG-TGGACACGGTGGCTTAGTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.6 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_0239 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA2ATCCAGAGTGACGCAGCA-CGACACGTTAGGTTGGTTAGGTTGGTTAGTTTCTTG-TGGACACGGTGGCTTA705'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry2.0 ± 0.6 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0240 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA3ATCCAGAGTGACGCAGCA-GTAGATGGTTTGGTTGGTGTGGTTTCCTACTGATGTTGGG-TGGACACGGTGGCTTAGTA775'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.3 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0241 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA4ATCCAGAGTGACGCAGCA-TTATGGGGTGTGGTGGGGGGTTAATGCGTTGGTTATCCG-TGTGGACACGGTGGCTTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry9.5 ± 2 × 10(1) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0242 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 1GCAATGGTACGGTACTTCC-CAACCCCGTCTATCACGTCGCTCTTGCGTTGGTTG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)7.5 ~ 10 nM (for ALS)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0243 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 2GCAATGGTACGGTACTTCC-CAACCCCGTCTATCACGTCGCTGTTGCGTTGGTTG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)7.5 ~ 10 nM (for ALS)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0244 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 3GCAATGGTACGGTACTTCC-CGCCGCCGCGTTCTCATCGCGTGATGTAGTGTCCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)17.5 ~ 20 nM (for ALS) and 30 ~ 35 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0245 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 4GCAATGGTACGGTACTTCC-CGCCGGCTTCTCTTGCCGTGATGTAGTGTCCG-CAAAAGTGCACGCTACTTTGCTAA755'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)15 nM (for ALS) and 20 ~ 25 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0246 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 5GCAATGGTACGGTACTTCC-CGGGGCCCGCTTCTGGTGCGGTGTACTAGTGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.ALSap-5 at 0.5 µM, about 90% of biofilm, 50.9% reduced pyocyanin secretions, as well as secretions of LasA protease and LasB elastase were inhibited.14Enzyme-Linked Immunosorbent Assay (ELISA)10 ~ 12.5 nM (for ALS) and 20 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/ABest CandidateN/AN/A
ABdb_0247 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 6GCAATGGTACGGTACTTCC-CGGGGCGGGCTGTCATGCCCATCCTACCGTGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)15 ~ 17.5 nM (for ALS) and 45 ~ 50 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0248 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 7GCAATGGTACGGTACTTCC-CGGGGCGGCCTGTGTTGGCCTACCTAGCGAGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)12.5 ~ 15 nM (for ALS) and 35 ~ 40 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0249 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 8GCAATGGTACGGTACTTCC-CGCTGCCCCCTGTCCTGGGTTAGCTAGCGAGAGCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.86% of pyocyanin secretion was inhibited by 6 µM of ALSap-8, and biofilm formation, and the secretions of LasA protease and LasB elastase were also decreased by 9.3%, 17.5%, and 19% respectively.14Enzyme-Linked Immunosorbent Assay (ELISA)10 nM (for ALS) and 25 ~ 30 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/ABest CandidateN/AN/A
ABdb_0250 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-17AGTATACGTATTACCTGCAGC-GAGGGAAGAAGGGCCAGCACAGATCAGATCAATCGCTCCG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.Lbi-17 showed maximum binding of 78.7 ± 12.38% with an LOD of the combined AMC-qPCR method of 1.8 log10 CFU/500μl L.monocytogenes and ranged from 26% to 77%.6Flow Cytometry35.7 ± 8.02 μMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0251 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-16AGTATACGTATTACCTGCAGC-AAATACTTTAGATCTAAGAGTGTTTCGAAAAGACAACAGA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0252 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-118AGTATACGTATTACCTGCAGC-TTGAATTAATAGATTAGTATACTTGGGAATCGTCCTAATA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0253 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-200AGTATACGTATTACCTGCAGC-AGGAAGACAAATTCCGCCAAAAAGTGGATATAACCAATAA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0254 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-203AGTATACGTATTACCTGCAGC-ATAGAGTAGAAGCTACACTACGTAATCACAGACAGATCCA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0255 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA15GGGAGCTCAGAATAAACGCTCAA-TACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.Display a LOD of 75 CFU/mL and a wide linear range from 10(2) to 10(7).8Fluorescence Spectroscopy48.74 ± 3.11 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0256 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA1GGGAGCTCAGAATAAACGCTCAA-GGGGGGCCTAGACTAGGGGGAGAGGGTGGGACGGT-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy86.41 ± 3.34 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0257 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA10GGGAGCTCAGAATAAACGCTCAA-GGGGCGGCGGCGGTGGTACGGGGTTGGGAGCGGGC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy203.12 ± 1.86 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0258 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA9GGGAGCTCAGAATAAACGCTCAA-GGCGTATGCGCAGCGAGGGCGGCCGGGCGACGTCG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy88.36 ± 3.60 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0259 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA8GGGAGCTCAGAATAAACGCTCAA-CCACGGGAACAACATCGTGGCAGGGACGAGCGTCCT-TTCGACATGAGGCCCGGATC795'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy120.91 ± 2.85 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0260 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA4GGGAGCTCAGAATAAACGCTCAA-GCGCGCTGCCGACGCGGGGGGGCTGATTAGCGTGG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy205.83 ± 1.65 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0261 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA13GGGAGCTCAGAATAAACGCTCAA-ACTGAGGGGCGGCGGACGGGATGGGAAATGTAGG-TTCGACATGAGGCCCGGATC775'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy299.94 ± 1.67 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0262 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA12GGGAGCTCAGAATAAACGCTCAA-TAGCGTGGGTAACCGTGTTGGGGGGTGCCACGGTC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy63.41 ± 3.39 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0263 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA1AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy92.17 ± 16.75 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0264 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA15/40AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.Limit of detection of 8.7±10(-3) μg/mL with linear range of 0.01-10 μg/mL SEA.12Fluorescence Spectroscopy48.57 ± 6.52 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0265 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.2AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy235.00 ± 79.05 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0266 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA3AGCAGCACAGAGGTCAGATG-TACGAGCGGTGGTTTTACCCTGCAATACTTTTGGCTGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0267 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA25AGCAGCACAGAGGTCAGATG-ATAGATTTTTTTCATGATTCTTCATTTTTTTATTTGAAAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0268 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA29AGCAGCACAGAGGTCAGATG-TATTATCTTACTACGTTGCATCCTACTTTTATAGTCCCCT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0269 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA30AGCAGCACAGAGGTCAGATG-GGATCTTCCCTCAATGTTTATTGTATATCTGTACTCGTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0270 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA10AGCAGCACAGAGGTCAGATG-TCCAATTCAATTCATTTCTGAACTTAGTCGGCACTTTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0271 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA4AGCAGCACAGAGGTCAGATG-CTCCAGCAGCCTCATAGATACGTCGTATCTATTGTTTCCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0272 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.1AGCAGCACAGAGGTCAGATG-AAGGTCTTACATCAATTTATATATTTTTCCAATATCTGTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0273 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20.27 ± 4.372 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0274 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2 Truncated 43-merGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGAC395'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)27.64 ± 4.784 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0275 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #2GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.The level of binding of #2 RNA aptamer to S. aureus was 1.6-fold higher than that of the control aptamer.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_0276 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #10GGGAGAGCGGAAGCGUGCUGGGCC-GUUAAUACGGUGUCUUUUUCGGUCGUGUAUAAAACGGAAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0277 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #14GGGAGAGCGGAAGCGUGCUGGGCC-UAGGACAGUUCGUCCUCAUUACAUCGCCGCCUAACACAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0278 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #16GGGAGAGCGGAAGCGUGCUGGGCC-UUAGAAGUAGCCUGCUACGCAUGGUCGACUCAAGAAUCG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0279 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #18GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0280 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #21GGGAGAGCGGAAGCGUGCUGGGCC-AGUCUGACACGUAGACAGUUCUAUUACUUACGUCGAGA-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0281 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #23GGGAGAGCGGAAGCGUGCUGGGCC-ACAGUGUUCUAAUGCGACAAUGGAGUCUGUGGCAAAGUGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0282 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #24GGGAGAGCGGAAGCGUGCUGGGCC-UCUCAGGCCGACAUUCUGAGAACGCGAGGCGUAUUGAAG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0283 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #26GGGAGAGCGGAAGCGUGCUGGGCC-UUCAAGUAGGGGCGGUUUACUAUCUGGAUCUUGUAGUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0284 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #07GGGAGAGCGGAAGCGUGCUGGGCC-ACUUGGGGACGACGAGUAGAUAGUAAGGUGGAGACCUGGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0285 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #06GGGAGAGCGGAAGCGUGCUGGGCC-UAUGACAUAAGGUGGGCUGGGAAGCUAGAGCAUGUAAGG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0286 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #05GGGAGAGCGGAAGCGUGCUGGGCC-GUGAAGAAAAAGGGGCGGAUUGGGUAGUAGGGAGGAGAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0287 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #04GGGAGAGCGGAAGCGUGCUGGGCC-AGGAUAGGGGAUGAAGAAAAAAAGAAGGGUGCCGUGGCGC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0288 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone G1GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0289 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA101ATTACTTACGCTATCTAAttt-GGGGGGTGGGTTGTTTGGGATGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0290 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA102ATTACTTACGCTATCTAAttt-GGGGGGGGAACATGTTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay3.5 nM (for B4) and 1.4 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0291 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA103ATTACTTACGCTATCTAAttt-GGGGCGGGACTTATTTGGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0292 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA104ATTACTTACGCTATCTAAttt-GGGGGTGGGTGCTTTTGTGGTGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0293 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA105ATTACTTACGCTATCTAAttt-TAGGGGTGGGTTCAATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0294 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA106ATTACTTACGCTATCTAAttt-GGGGGGCGGGTATTAATGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0295 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA107ATTACTTACGCTATCTAAttt-GTTTTCGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0296 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA108ATTACTTACGCTATCTAAttt-GCACTAAAGGGGAGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0297 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA109ATTACTTACGCTATCTAAttt-TTGCTTTAGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay7.7 nM (for B4) and 4.1 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0298 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA110ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0299 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA111ATTACTTACGCTATCTAAttt-GCCCGATGGGGGTGGCGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0300 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G02ATTACTTACGCTATCTAAttt-TTGCTGTAGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Displayed the highest specificity, exhibiting a 36% higher specificity index than that of the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0301 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G19ATTACTTACGCTATCTAAttt-TTTTTCGGGGGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0302 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G14ATTACTTACGCTATCTAAttt-TTGCTTTAGAGGAGGCGGGTGGAG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0303 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G11ATTACTTACGCTATCTAAttt-TCGGTGATGGGGAGGAGGCGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0304 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G03ATTACTTACGCTATCTAAttt-GTTTTATGGGGGTGGCGTGTGGCG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0305 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G06ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGCGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0306 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G10ATTACTTACGCTATCTAAttt-TTACTTTTGGGGGGGCGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0307 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G02ATTACTTACGCTATCTAAttt-GCACGTTTGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0308 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G09ATTACTTACGCTATCTAAttt-TTTTTCGAGGGGAGATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0309 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G20ATTACTTACGCTATCTAAttt-GCGCGAGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0310 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G04ATTACTTACGCTATCTAAttt-GCCCGCGTCGGGGGGTGGGGGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0311 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G01ATTACTTACGCTATCTAAttt-TCGCTATGGGGGTGGCGGCTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0312 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G17ATTACTTACGCTATCTAAttt-TAGCTTTAGGGGTCGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0313 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G08ATTACTTACGCTATCTAAttt-GAGCTTTAGAGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0314 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G04ATTACTTACGCTATCTAAttt-TTCCTTGGGGAGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0315 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G06ATTACTTACGCTATCTAAttt-GTTTTCGGGGGCTGGTGGTTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0316 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G09ATTACTTACGCTATCTAAttt-TGGCTCGGGGGGTGTTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0317 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE1GCAATGGTACGGTACTTCC-ACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy12.4 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0318 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE2GCAATGGTACGGTACTTCC-CCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy25.2 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0319 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE10GCAATGGTACGGTACTTCC-GTTGCACTGTGCGGCCGAGCTGCCCCCTGGTTTGTGAATACCCTGGG-CAAAAGTGCACGCTACTTTGCTAA905'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy14.2 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0320 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE12GCAATGGTACGGTACTTCC-GCGAGGGCCAACGGTGGTTACGTCGCTACGGCGCTACTGGTTGAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy16.8 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0321 https:doi.org10.1007s13765-013-3019-72013Potential of fluorophore labeled aptamers for Pseudomonas aeruginosa detection in drinking waterP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 10145)Whole cellDevelop a fluorophore-labelled aptamer to detect P. aeruginosa in drinking water.The limit of detection for P. aeruginosa was 5.07 cells/mL, with a linear dynamic range of 5.64 to 100 cells/mL.N/AN/AN/ABiosensorN/AFITC LabeledN/AN/AN/AN/AN/A
ABdb_0322 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS 1ATAGGAGTCACGACGACCAGAA-CGGAACTAGCGTTTAAATGCCAGGACTGAAGTAGGCAGGG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellIdentify an aptamer targeted against Shigella dysenteriae and develop a sandwich-type fluorescent bioassay for quantification.Obtained linear range between 10(2)-10(7) cfu/mL of S. dysenteriae, and the limit of detection was 50 cfu/mL8Fluorescence Binding Assay23.47 ± 2.48 nMDetectionWhole Cell-SELEX5'-FAM Labeled and BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0323 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS12ATAGGAGTCACGACGACCAGAA-CCTGGCGGGTCCCGGGGTAAACGGCACAAACGATAAAGAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0324 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS2ATAGGAGTCACGACGACCAGAA-AAGATGACACTTGGCAGCCGCCTCGAGTGTCCTACACGCA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0325 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS8ATAGGAGTCACGACGACCAGAA-GCCAGATGAGGCCGGCAGGGCCCAAGTGTTGCTCGGGCTA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0326 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS21ATAGGAGTCACGACGACCAGAA-TGCACGGGACAAGAGTACACGCCGATTGCCAGGCACAGTG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding Assay75.49 ± 3.74 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0327 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS10ATAGGAGTCACGACGACCAGAA-GGGGAAGCCGATCAGGCCAATCATTGAGGGTGAACTAGCT-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0328 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS19ATAGGAGTCACGACGACCAGAA-TTATCGGCTGGCAAAACTGCGGCTGGAGCTCACAACTAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0329 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS13ATAGGAGTCACGACGACCAGAA-TCAGCGAGGGCCAATTAGAAGGGTACTCATGTCTGTGGAC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0330 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS24ATAGGAGTCACGACGACCAGAA-CCAGGCGGAATGTGTCTTCGTTTTGCGAGTGTTAAGGGCG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0331 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-7GTATACGTATTACCTGCAGC-CTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.N/A10Flow Cytometry1.73±0.54 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0332 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-46GTATACGTATTACCTGCAGC-CTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.Achieved a Lower Limit of Detection (LOD) of 10(2)-10(3) CFU in a 290-μl sample, and the mean capture efficiency ranged from 3.6% to 12.6%.10Flow Cytometry0.74±0.20 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0333 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA1GGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC71N/AssRNASalmonella Typhi (CECT 409)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 0.2 CFU/mL (1 CFU in 5 mL PBS) to 10(6) CFU/mL of ST and LOD of 0.2 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0334 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA2GGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) (CECT 675)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 4 to 10(4) CFU/mL and LOD of 4 CFU/mL, and the signal was stable after 120 s.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0335 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA3GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 8 × 10(2) CFU/mL–10(8) CFU/mL with LOD of 8 x10(2) CFU/mL and the signal was stable for at least one hour.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0336 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt1ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer-modified QDs (QD-apt) were developed to selectively capture and simultaneously detect the target bacteria.For V. parahaemolyticus cells, a linear range of 3.4 × 10(4) to 3.4 × 10(7) cfu/mL was obtained with QD 535-apt 1-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0337 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt2ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-modified Quantum Dots (QD-apt) were developed for selective bacterial capture.For S. typhimurium, a linear range obtained between the concentrations 3.8 × 10(4) to 3.8 × 10(7) cfu/mL was obtained with QD 585-apt 2-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0338 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionL. acidophilus (FALA)ATCCGTCACACCTGCTCTACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTATGGTGTTGGCTCCCGTAT73N/AssDNALactobacillum Acidophilius (ATCC 4356)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 11.0 cfu/mL (L. acidophilus).N/AN/A13 nMBiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0339 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. aureus (FASA)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 800.0 cfu/mL (S. aureus) and linear ranges of 10(4)–10(6) cfu/mL.N/AN/A35 nMBiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0340 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. enterica (FASE)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 61.0 cfu/mL (S. enterica) and linear ranges of 42.2–675.0 cfu/mL.N/AN/AN/ABiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0341 https://doi.org/10.1016/j.snb.2013.01.0622013A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteinsECA IGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGC37N/AssDNAEscherichia Coli (E. Coli) (ATCC® 8739™)Outer membrane protein (OMP)Developed a label-free aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs).Demonstrated in a dynamic detection range of 1 × 10(-7)–2 × 10(-6) M.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0342 https://doi.org/10.1016/j.snb.2013.01.0622013A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteinsECA IIACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA37N/AssDNAEscherichia Coli (E. Coli) (ATCC® 8739™)Outer membrane protein (OMP)Developed a label-free aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs).Demonstrated in a dynamic detection range of 1 × 10(-7)–2 × 10(-6) M.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1970 233816902013Selection of aptamers against inactive Vibrio alginolyticus and application in a qualitative detection assayPool of enriched aptamersN/AN/A5'-TCAGTCGCTTCGCCGTCTCCTTC-N35-GCACAAGAGGGAGACCCCAGAGGG-3'ssDNAVibrio AlginolyticusWhole cell (Inactivated)Identify aptamers against and qualitatively detect inactive Vibrio alginolyticus using PCR.V. alginolyticus could be detected at 100 cells/ml.15Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)27.5 ± 9.2 nMDetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_1971 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4936–4N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay3.1 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1972 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4939–280N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay1.6 nMDetectionSELEX5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1973 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4943–51N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay1.3 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1974 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5564–89N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay9.8 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1975 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5570–54N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay6.6 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1976 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4937–55N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.76 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1977 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4938–17N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.45 nMDetectionSELEX5-benzylaminocarbonyl-dU (BndU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1978 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4940–23N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay0.07 nMDetectionSELEX5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1979 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4944–30N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.08 nMDetectionSELEX5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1980 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5566–74N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.04 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1981 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5573–4N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.07 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1982 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4758–6N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.22 nMDetectionSELEX5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1983 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5574–49N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay1.1 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1984 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5579–11N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.05 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1985 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5579–12N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay0.69 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1986 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5556–51N/AN/AN/AssDNAClostridium DifficileBinary toxin, B chain (CdtB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay6.9 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1987 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5556–67N/AN/AN/AssDNAClostridium DifficileBinary toxin, B chain (CdtB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay4.4 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A